Human skin identification using specific gene marker at different storage temperatures

Abstract

Background: Late cornified envelope 1D (LCE1D), skin-specific mRNA, is used in forensic researche on human skin cell identification. Before using (LCE1D) in criminal casework, the impact of storage conditions on its expression should be measured and assessed. To detect the effects of storage temperature and duration, skin swabs were collected from six volunteers and stored at room temperature (25°C) and warmer temperature (40°C) for 5 days. Results: The (LCE1D) expressions, detected via Real Time PCR, were significantly diminished by increased storage duration. While there were also decreased (LCE1D) expressions among warmer temperature samples, this difference was not statistically significant. Conclusion: Our results suggest that forensic investigators should consider sample collection time prior to result interpretation.

Histological and miRNAs postmortem changes in incisional wound

“…The skin samples in our study were examined after death as postmortem samples of perimortal wound. Moreover, the condition of storage environment can greatly affect the speed of bio-molecular and histological changes (Ibrahim 2018).…”

Real-time temperature monitoring and alarm system for biological samples storage based on FPGA

“…Storage of sweat samples at 25℃ for more than 7 days affects sweat lactate and ammonia concentrations but can be extended to 28 days at -20℃ [12] . The expression level of the Late Cornified Envelope 1D (LCE1D) gene in human skin swabs stored at 25℃ is significantly higher than those stored at 40℃ [13] . To prevent storage temperature from affecting the quality of biological samples, it is crucial to design a temperature monitoring and alarm system.…”