Human serum, cysteine and histidine inhibit the oxidation of low density lipoprotein less at acidic pH

Patterson, Rebecca A.; Leake, David S.

doi:10.1016/s0014-5793(98)01002-3

Cited by 51 publications

(16 citation statements)

References 26 publications

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“…The enzyme reaction in the tube, which contains NADPH, reduced glutathione, sodium azide, and glutathione reductase, was initiated by addition of hydrogen peroxide, and the change in absorbance at 340 nm was monitored by a spectrophotometer. Activity was given as units per gram of hemoglobin in erythrocyte samples (Patterson and Leacke 1998).…”

Section: Gpx Activitymentioning

confidence: 99%

Biochemical assessment of oxidative status versus liver enzymes in patients with chronic fascioliasis

2014

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The aim of this study was to examine the oxidative status in Egyptian patients suffering chronic fascioliasis. The relationship between serum malondialdehyde (MDA) levels, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) activities was investigated in relation to the level of liver enzymes; ALT and AST compared to healthy controls. Twenty patients versus ten controls were included in the study. Among cases the MDA, CAT, AST and ALT were higher than controls, while SOD and GPX higher values were present among controls. There was a highly significant difference between cases and controls as regard MDA, CAT, SOD, GPX, and AST, and a significant difference regarding ALT. The findings of increased serum lipid peroxidation and decreased antioxidant enzymes in erythrocytes of chronic fascioliasis patients indicated the presence of persistent inflammation and oxidative stress which confirms the underlying pathogenesis and reflected the stage of infection providing a baseline data for comparison between normal and infected patients guided by the level of liver enzymes in relation to oxidative status.

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Section: Gpx Activitymentioning

confidence: 99%

Biochemical assessment of oxidative status versus liver enzymes in patients with chronic fascioliasis

2014

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“…A possible role of the highly reactive oxygen free radicals in the pathogenesis of parasitic infections has been an active area of research over the past ten years (Biswas et al, 1997;Erel et al, 1997;Oliveira and Cechini, 2002). The main targets of free oxygen radical activity are polyunsaturated fatty acids (PUFAs) in membrane phospholipids, the modification of which result in disorganization of the cellular framework and function (Patterson and Leacke, 1998).…”

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confidence: 99%

Malondialdehyde levels in serum of dogs infected with Babesia canis

Crnogaj¹,

Petlevski²,

Mrljak³

et al. 2010

Vet. Med.

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Babesiosis is a common tick born disease of dogs in tropical and subtropical regions of the world caused by different species of Babesia. The aim of the present study was to confirm the presence of oxidative stress by examining serum malondialdehyde (MDA), an end product of lipid peroxidation, in 35 dogs naturally infected with Babesia canis (B. canis). MDA was examined in 14 healthy dogs as well. Blood smears were prepared from peripheral blood and they showed the presence of B. canis in infected dogs. B. canis was confirmed using the PCR (Polymerase chain reaction) method. On the basis of clinical and laboratory data the 35 infected dogs were clinically classified into two groups, complicated (seven dogs) and uncomplicated (28 dogs). The noted complications were renal dysfunction (5/7), hepatic dysfunction (3/7), muscular involvement (2/7) and ARDS (1/7). Levels of blood urea nitrogen concentration (BUN), creatinin, total bilirubin, alanin aminotranspherase (ALT), alkaline phosphatase (AP), and gammaglutamil aminotranspherase (GGT) were significantly increased in dogs with complicated versus uncomplicated babesiosis. Furthermore the uncomplicated group of dogs was, depending on the severity of anaemia, classified as suffering from severe, moderate or mild disease. Levels of serum MDA were significantly higher in sick dogs (36.90 μmol/l ± 13.95) than healthy animals (8.13 μmol/l ± 1.78). There was no significant statistical difference in serum MDA levels between dogs with complicated (38.48 μmol/l ± 12.11) and uncomplicated babesiosis (36.50 μmol/l ± 14.55). Comparison of the groups based on the severity of anaemia showed that there was no significant statistical difference in serum MDA levels between them. The study demonstrated the involvement of oxidative damage in dogs naturally infected with B. canis.

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“…A protein assay, conducted as described above, showed that between 98-100% (n ϭ 3) of the apolipoprotein was removed. Previous work (23) has shown an absence of lipoprotein bands after electrophoresis of similarly treated serum on agarose gels, and minimal removal of other protein from the serum.…”

Section: Preparation Of Lipid-deficient Plasmamentioning

confidence: 87%

Effect of fluid mechanical stresses and plasma constituents on aggregation of LDL

Talbot

Rio

Weinberg

2003

Journal of Lipid Research

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LDL aggregates when exposed to even moderate fluid mechanical stresses in the laboratory, yet its half-life in the circulation is 2-3 days, implying that little aggregation occurs. LDL may be protected from aggregation in vivo by components of plasma, or by a qualitative difference in flows. Previous studies have shown that HDL and albumin inhibit the aggregation induced by vortexing. Using a more reproducible method of inducing aggregation and assessing aggregation both spectrophotometrically and by sedimentation techniques, we showed that at physiological concentrations, albumin is the more effective inhibitor, and that aggregation is substantially but not completely inhibited in plasma. Heat denatured and fatty-acid-stripped albumin were more effective inhibitors than normal albumin, supporting the idea that hydrophobic interactions are involved. Aggregation of LDL in a model reproducing several aspects of flow in the circulation was 200-fold slower, but was still inhibited by HDL and albumin, suggesting similar mechanisms are involved. Within the sensitivity of our technique, LDL aggregation did not occur in plasma exposed to these flows. Thus, as a result of the characteristics of blood flow and the inhibitory effects of plasma components, particularly albumin, LDL aggregation is unlikely to occur within the circulation.

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Human serum, cysteine and histidine inhibit the oxidation of low density lipoprotein less at acidic pH

Cited by 51 publications

References 26 publications

Biochemical assessment of oxidative status versus liver enzymes in patients with chronic fascioliasis

Biochemical assessment of oxidative status versus liver enzymes in patients with chronic fascioliasis

Malondialdehyde levels in serum of dogs infected with Babesia canis

Effect of fluid mechanical stresses and plasma constituents on aggregation of LDL

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