Human serum antibodies against native DNA detected by an indirect immunoperoxidase spot technique

Matre, R.; Helgeland, Svein M.; Tönder, O.

doi:10.1016/0022-1759(74)90018-0

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…Others (3,4,6,(10)(11)(12)(13) have reported on the principle of using immunoenzyme techniques for the detection of serum antibodies and antigens. However, these procedures as reported are not ideally suited for large-scale economic screening of animal and human sera for antibodies (or antigens) because of the fairly long incubation times required (60 min to several hours), types of reaction vehicles employed (such as individual polystyrene tubes or culture dishes), or the relatively large amounts of reagents required in macrotechniques.…”

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confidence: 99%

Rapid micromethod of screening for antibodies to disease agents using the indirect enzyme-labeled antibody test

Saunders¹,

Clinard²

1976

J Clin Microbiol

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We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.

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confidence: 99%