Human seminal proteinase and prostate-specific antigen are the same protein

Abstract

Human seminal proteinase and prostate-specific antigen (PSA) were each isolated from human seminal fluid and compared. Both are glycoproteins of 32-34 kDa with protease activities. Based on some physicochemical,enzymatic and immunological properties,it is concluded that these proteins are in fact identical. The protein exhibits properties similar to kallikrein-like serine protease, trypsin,chymotrypsin and thiol acid protease. Tests of the activity of the enzyme against some potential natural and synthetic substrates showed that bovine serum albumin was more readily hydrolysed than casein. The results of this study should be useful in purifying and assaying this protein. Based on published studies and the present results,the broad proteolytic specificity of human seminal proteinase suggests a role for this protein in several physiological functions.

Proteases and Proteases Inhibitors of Semen - A Review

“…A 32-34 kDa prostate-specific antigen synthesized in epithelial cells of prostate gland is isolated from human seminal fluid after it has been secreted (Waheed et al, 2008). A 52 kDa protein expressed on the seminal vesicle, seminogelin is known to play an important role in spontaneous coagulation and liquefaction of human semen (Matsuda et al, 1994).…”