Human Salivary Glycosidases

Development and application of assay for determining β-glucosidase activity in human saliva

“…This relatively low prevalence of human salivary β-glucosidase activity is consistent with the findings of Menguy et al [24] (1 from 5 individuals) and Zambon et al [23] (0 from 5 individuals). The aforementioned study by Walle et al [35] measured the rates of enzymatic hydrolysis in 17 individuals; three individuals showed very high rates (defined by consumption of substrate), one individual showed no hydrolysis and the remainder fell within a range of barely detectable to moderate levels of hydrolysis.…”

“…Few studies appear on the possible activity and prevalence of β-glucosidase in human saliva. Menguy et al [24] suggested that approximately one in five people possess β-glucosidase in their saliva; however, the sample size of their study was quite small. Later, Arnaldos et al [25] modified a spectrophotometric assay for strawberry callus protein for potential application in determining β-glucosidase in human saliva.…”

Confirmation of Metachromatic Leukodystrophy and Fucosidosis by Enzyme Analysis of Saliva

“…Except for T ay-Sachs disease (Singer et al 1973), enzyme assays in sa1iva have not been used for the detection of storage diseases. However, 1ysosoma1 enzymes such as glycosidases (Menguy et al 1970), arylsu1phatase (Rinderknecht et al 1970), and acid phosphatase (Beeley and Ghisholm 1976) have been determined previous1y in sa1iva for other purposes.…”

α‐D‐mannosidase activity in human saliva and rate of production of enzymes by parotid and submandibidar glands

“…Acidic, ititermediate and neutral a-Dsponsible for hydrolysis of a-mannoside mannosidases are also found in human plasbonds in mticopolysaccharides and glyco-nia and serum. a-D-mannosidase activity has human saliva has been assayed at an acidic been found in a number of human and ani-pH and found in whole saliva, iti whole salimal tissues and also in human biological va supernatant after centrifugation and also fluids such as cerebrospinal fluid, urine, plas-in submandibular saliva (8)(9)(10). a-D-mannosidase activity has human saliva has been assayed at an acidic been found in a number of human and ani-pH and found in whole saliva, iti whole salimal tissues and also in human biological va supernatant after centrifugation and also fluids such as cerebrospinal fluid, urine, plas-in submandibular saliva (8)(9)(10).…”

“…a-D-Mannosidase activity in proteins (1,2). ma, tear fluid, and saliva (3)(4)(5)(6)(7)(8). ma, tear fluid, and saliva (3)(4)(5)(6)(7)(8).…”