Human prostate cancer precursors and pathobiology

Marzo, Angelo M. De; Meeker, Alan K.; Zha, Shan; Luo, Jun; Nakayama, Masashi; Platz, Elizabeth A.; Isaacs, William B.; Nelson, William G.

doi:10.1016/j.urology.2003.09.053

Cited by 171 publications

(115 citation statements)

References 62 publications

(38 reference statements)

Supporting

Mentioning

106

Contrasting

Unclassified

Order By: Relevance

“…2 A new molecular marker called racemase or AMACR (alpha-methyl-acyl-CoA racemase, P504S) was recently applied to separate benign from neoplastic acini. 2,4,23 However, a high proportion of high-grade PIN, as well as some foci of adenosis and some entirely benign glands are also positive for this marker. 6 Because of the cytoplasmic localization of P504S and nuclear localization of p63, Tacha and Miller hypothesized that a cocktail of these two antibolies might allow simultaneous demonstration of P504S and p63 using a single immunostain.…”

Section: Discussionmentioning

confidence: 99%

Periacinar clefting and p63 immunostaining in prostatic intraepithelial neoplasia and prostatic carcinoma

Krušlin

Tomas

Čviko

et al. 2006

Pathol. Oncol. Res.

View full text Add to dashboard Cite

The aim of the present study was to correlate the presence and extent of retraction clefting and the expression of p63 in neoplastic glands and glands with prostatic intraepithelial neoplasia (PIN) in needle core biopsies.We analyzed needle core biopsies from 28 patients with PIN and 41 patients with adenocarcinoma. Neoplastic glands and those with PIN were analyzed on high power field (400x) and classified in three groups. Immunohistochemical staining was performed following Microwave Streptavidin ImmunoPeroxidase (MSIP) protocol on DAKO TechMate Horizon automated immunostainer using antibodies to p63 (4A4, M7247, DAKO, Denmark).Periacinar retraction clefting was significantly more prominent in prostatic carcinoma compared to PIN (p<0.0001) and nonneoplastic glands (p<0.0001). There was no difference between normal glands and PIN regarding clefting (p=0.8064). P63 was positive around whole circumference in 12 out of 28 cases with PIN, and discontinuously positive in remaining 16 PIN cases suggesting initial disruption of basal cell layer. P63 immunostaining was also positive in all nonneoplastic glands, and negative in all carcinoma cases.We conclude that retraction clefting was associated with cancer and lack of basal cells, but not with PIN. The relationship between clefting and p63 immunostaining in prostatic cancer should be further analyzed.

show abstract

Section: Discussionmentioning

confidence: 99%

Periacinar clefting and p63 immunostaining in prostatic intraepithelial neoplasia and prostatic carcinoma

Krušlin

Tomas

Čviko

et al. 2006

Pathol. Oncol. Res.

View full text Add to dashboard Cite

show abstract

“…Prostate cancer is the most frequent form of cancers in males in most western countries; 36 an estimation of 1 in 6 men in the US is diagnosed with prostate cancer. 37,38 However, the mechanism of development and progression remain poorly understood. 37,38 One of the contributing factors is the challenge of filtering these cells from mixtures, which we hope to alleviate in this work.…”

Section: Cell Filtrationmentioning

confidence: 99%

Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel

et al. 2014

View full text Add to dashboard Cite

Cell filtration is a critical step in sample preparation in many bioapplications. Herein, we report on a simple, filter-free, microfluidic platform based on hydrodynamic inertial migration. Our approach builds on the concept of two-stage inertial migration which permits precise prediction of microparticle position within the microchannel. Our design manipulates equilibrium positions of larger microparticles by modulating rotation-induced lift force in a low aspect ratio microchannel. Here, we demonstrate filtration of microparticles with extreme efficiency (>99%). Using multiple prostate cell lines (LNCaP and human prostate epithelial tumor cells), we show filtration from spiked blood, with 3-fold concentration and >83% viability. Results of a proliferation assay show normal cell division and suggest no negative effects on intrinsic properties. Considering the planar low-aspect-ratio structure and predictable focusing, we envision promising applications and easy integration with existing lab-on-a-chip systems. V C 2014 AIP Publishing LLC.

show abstract

“…Atrophic epithelium in PAH has recently been proposed to be a precursor to PIN and PCA (De Marzo et al, 1999;Putzi and De Marzo, 2000;Shah et al, 2001;reviewed in De Marzo et al, 2003;Nelson et al, 2003). Several studies have identified focal areas of epithelial atrophy and have suggested their importance in prostatic carcinogenesis (Franks, 1954;Gardner and Bennett, 1992;De Marzo et al, 2003;Nelson et al, 2003). It has also been reported that atrophy of prostatic glands is common in middle-aged and elderly men (Gardner and Culberson, 1987;McNeal, 1988).…”

Section: Introductionmentioning

confidence: 99%

Detection and expression of human BK virus sequences in neoplastic prostate tissues

2004

View full text Add to dashboard Cite

Human prostate cancer precursors and pathobiology

Cited by 171 publications

References 62 publications

Periacinar clefting and p63 immunostaining in prostatic intraepithelial neoplasia and prostatic carcinoma

Periacinar clefting and p63 immunostaining in prostatic intraepithelial neoplasia and prostatic carcinoma

Modulation of rotation-induced lift force for cell filtration in a low aspect ratio microchannel

Detection and expression of human BK virus sequences in neoplastic prostate tissues

Contact Info

Product

Resources

About