1976
DOI: 10.1111/j.1432-1033.1976.tb10645.x
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Human‐Polymorphonuclear‐Leucocyte Neutral Protease and Its Inhibitor

Abstract: Human polymorphonuclear leucocytes were obtained from the synovial fluids of patients with inflamed knee joints suffering either from Reiter's syndrome or from rheumatoid arthritis. The polymorphonuclear leucocytes were collected by gentle centrifugation followed by disruption and their subcellular fractionation by centrifugation in 0.34 M sucrose to provide a granule fraction and a post-granule supernatant fraction. 0.5 M KCl extraction of the granule fraction yielded neutral protease activity, similar to try… Show more

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Cited by 13 publications
(2 citation statements)
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“…Work with sonicates of tumor cells demonstrated the presence of latent forms of neutral protease and collagenase [3], activated by trypsin, and an inhibitor of this enzyme in the post-granule supernatant fraction [4]. This inhibitor was shown to inactivate trypsin [4] by the assay system previously described for the inhibitor of the polymorphonuclear leucocyte neutral protease [5]. We wish to present evidence for the presence of one or more significant disulphide bond(s) within both trypsin and the neutral protease required for the maintenance of the active form of these enzymes and to report the common Abbreviations.…”
mentioning
confidence: 99%
“…Work with sonicates of tumor cells demonstrated the presence of latent forms of neutral protease and collagenase [3], activated by trypsin, and an inhibitor of this enzyme in the post-granule supernatant fraction [4]. This inhibitor was shown to inactivate trypsin [4] by the assay system previously described for the inhibitor of the polymorphonuclear leucocyte neutral protease [5]. We wish to present evidence for the presence of one or more significant disulphide bond(s) within both trypsin and the neutral protease required for the maintenance of the active form of these enzymes and to report the common Abbreviations.…”
mentioning
confidence: 99%
“…This is in contrast to human leukocytes, in which only small amounts of inhibitors have been described (10).…”
Section: Introductionmentioning
confidence: 93%