Human platelet lysate-derived exosomes are superior to the lysate at increasing collagen deposition in a rat model of intrinsic aging

Rosmarwati, Ervina; Ellistasari, Endra Yustin; Kusumawardani, Arie; Julianto, Indah; Widhiati, Suci; Setyawan, Novan Adi; Yanuar, Frieda

doi:10.7324/japs.2023.125707

Cited by 2 publications

(2 citation statements)

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“…In addition, growth factors (GFs) are not directly present in the liquid lysate but are encapsulated within exosomes and vesicles in HPL and FBS. Alongside GFs, exosomes and vesicles also contain miRNA, which plays a significant role in cellular communication [46]. In addition to the impact of differing growth factor concentrations, conducting exosomal small RNA-seq can offer additional insights into the composition of exosomes or small vesicles.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth

Yoon,

Vu,

Lee

et al. 2024

Cells

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In recent years, there has been a surge in demand for and research focus on cell therapy, driven by the tissue-regenerative and disease-treating potentials of stem cells. Among the candidates, dental pulp stem cells (DPSCs) or human exfoliated deciduous teeth (SHED) have garnered significant attention due to their easy accessibility (non-invasive), multi-lineage differentiation capability (especially neurogenesis), and low immunogenicity. Utilizing these stem cells for clinical purposes requires careful culture techniques such as excluding animal-derived supplements. Human platelet lysate (hPL) has emerged as a safer alternative to fetal bovine serum (FBS) for cell culture. In our study, we assessed the impact of hPL as a growth factor supplement for culture medium, also conducting a characterization of SHED cultured in hPL-supplemented medium (hPL-SHED). The results showed that hPL has effects in enhancing cell proliferation and migration and increasing cell survivability in oxidative stress conditions induced by H2O2. The morphology of hPL-SHED exhibited reduced size and elongation, with a differentiation capacity comparable to or even exceeding that of SHED cultured in a medium supplemented with fetal bovine serum (FBS-SHED). Moreover, no evidence of chromosome abnormalities or tumor formation was detected. In conclusion, hPL-SHED emerges as a promising candidate for cell therapy, exhibiting considerable potential for clinical investigation.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth

Yoon,

Vu,

Lee

et al. 2024

Cells

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show abstract

“…In addition, exosomes can also act on melanocytes and reverse hyperpigmentation caused by UV irradiation or natural aging [ 353 , 351 ]. Moreover, exosomes have a positive impact on keratinocytes and fibroblasts, leading to increased skin hydration [ 350 ] and a reduction in the expression of collagenase, including MMP-1, and proinflammatory cytokines [ 350 , 349 ]. The presented findings are encouraging regarding exosome therapy for skin regeneration and its potential short-term application in human patients.…”

Section: Exosomes In Regenerative Medicine: Tissue-specific Applicationsmentioning

confidence: 99%

Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade

Moghassemi,

Dadashzadeh,

Sousa

et al. 2024

Bioactive Materials

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Human platelet lysate-derived exosomes are superior to the lysate at increasing collagen deposition in a rat model of intrinsic aging

Cited by 2 publications

References 0 publications

Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth

Evaluation of Human Platelet Lysate as an Alternative to Fetal Bovine Serum for Potential Clinical Applications of Stem Cells from Human Exfoliated Deciduous Teeth

Extracellular vesicles in nanomedicine and regenerative medicine: A review over the last decade

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