Human placenta hydrogel reduces scarring in a rat model of cardiac ischemia and enhances cardiomyocyte and stem cell cultures

Francis, Michael P.; Breathwaite, Erick; Bulysheva, Anna; Varghese, Frency; Rodriguez, Rudy U; Dutta, Sucharita; Semenov, Iurii; Ogle, Rebecca; Huber, Alexander; Tichy, Alexandra Madelaine; Chen, Silvia; Zemlin, Christian W.

doi:10.1016/j.actbio.2016.12.027

Cited by 57 publications

(56 citation statements)

References 34 publications

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“…This evaluation of both acute and chronic MI shows the effectiveness of the modulated scaffolds in repairing cardiovascular tissue for both immediate and extended treatment. Francis et al created soluble dECM based on human placenta (hpECM) for use as an injectable scaffold for MI treatment . The hpECM was shown to contain a complex composition of reparative ECM components such as agrin, sGAGs, fibronectin, vitronectin, and various collagen and laminin forms.…”

Section: In Vivo Evaluation Of Decm Therapies For Cardiovascular Tissmentioning

confidence: 99%

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Bejleri

Davis

2019

Adv Healthcare Materials

150

105

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Decellularized extracellular matrix (dECM) is a promising biomaterial for repairing cardiovascular tissue, as dECM most effectively captures the complex array of proteins, glycosaminoglycans, proteoglycans, and many other matrix components that are found in native tissue, providing ideal cues for regeneration and repair of damaged myocardium. dECM can be used in a variety of forms, such as solid scaffolds that maintain native matrix structure, or as soluble materials that can form injectable hydrogels for tissue repair. dECM has found recent success in many regeneration and repair therapies, such as for musculoskeletal, neural, and liver tissues. This review focuses on dECM in the context of cardiovascular applications, with variations in tissue and species sourcing, and specifically discusses advances in solid and soluble dECM development, in vitro studies, in vivo implementation, and clinical translation.

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Section: In Vivo Evaluation Of Decm Therapies For Cardiovascular Tissmentioning

confidence: 99%

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Bejleri

Davis

2019

Adv Healthcare Materials

150

105

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“…Decellularized matrix scaffolds have been widely utilized to repair ischemic tissues and promote tissue regeneration [20][21][22]. e prevailing advantage of decellularized matrix scaffolds is their similar properties with the native extracellular matrix, thus, providing a proper microenvironment for cell survival, retention, and engraftment of transplanted stem cells [23].…”

Section: Discussionmentioning

confidence: 99%

Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

Gao

Feng

Cui

et al. 2020

BioMed Research International

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Bone marrow-derived stem/progenitor cells have been utilized for cardiac or vascular repair after ischemic injury, but they are subject to apoptosis and immune rejection in the ischemic site. Multiple scaffolds were used as delivery tools to transplant stem/ progenitor cells; however, these scaffolds did not show intrinsically antiapoptotic or anti-inflammatory properties. Decellularized aortic scaffolds that facilitate cell delivery and tissue repair were prepared by removing cells of patient-derived aortic tissues. Scanning electron microscopy (SEM) showed cells attached well to the scaffold after culturing for 5 days. Live/dead staining showed most seeded cells survived at day 7 on a decellularized aortic scaffold. Ki67 staining demonstrated that decellularized aortic scaffold promoted proliferation of bone marrow-derived CD34+ progenitor cells. Apoptosis of CD34+ progenitor cells induced by H 2 O 2 at high concentration was significantly alleviated in the presence of decellularized aortic scaffolds, demonstrating a protective effect against oxidative stress-induced apoptosis. Furthermore, decellularized aortic scaffolds significantly reduced the expression of proinflammatory cytokines (IL-8, GM-CSF, MIP-1β, GRO-α, Entoxin, and GRO) concurrently with an increase in anti-inflammatory cytokines (IL-2 and TGF-β) released from CD34+ progenitor cells when exposed to H 2 O 2 at low concentration. Finally, neovascularization was observed by H&E and immunohistochemical staining 14 days after the decellularized aortic scaffolds were subcutaneously implanted in nude mice. is preclinical study demonstrates that the use of a decellularized aortic scaffold possessing antiapoptotic and anti-inflammatory properties may represent a promising strategy for cardiovascular repair after ischemic injury.

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“…Among them, the application of ECM (Extracellular matrix) hydrogel is the representative of natural hydrogel (Francis et al, 2017). Once the nanofiber hydrogel is formed by thermal induction at physiological temperature, the decellularized myocardial matrix hydrogels are possible to quickly create a natural cellular microenvironment for heart tissue and promote myocardial cell repair (Stoppel et al, 2016).…”

Section: Natural Hydrogelmentioning

confidence: 99%

“…To the begin, natural hydrogels were attracting attention because of their non-toxicity, immunogenicity, and excretion of metabolites (Li L. et al, 2019), such as. However, due to the lack of effective extraction methods (Francis et al, 2017), ECM were gradually replaced by other natural hydrogels, such as hyaluronic acid hydrogels (Yoon et al, 2009) (an immunological linear neutral polysaccharide with multiple acid and hydroxyl groups, which can be modified into different forms of hydrogels, including soft or hard hydrogels, as well as nanoparticles and electrospinning), chitosan natural hydrogels (which had good compatibility with macrophages and antioxidant properties and degradability) (Aussel et al, 2019), sodium alginate hydrogels (Hadley and Silva, 2019), and so on. On the other hand, synthetic hydrogels have been attached much importance since their strong mechanical properties and various and controllable function by physical and chemical means (Pena et al, 2018).…”

Section: Summary and Perspectivementioning

confidence: 99%

Injectable Hydrogel-Based Nanocomposites for Cardiovascular Diseases

Liao

Yang

Deng

et al. 2020

Front. Bioeng. Biotechnol.

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Cardiovascular diseases (CVDs), including a series of pathological disorders, severely affect millions of people all over the world. To address this issue, several potential therapies have been developed for treating CVDs, including injectable hydrogels as a minimally invasive method. However, the utilization of injectable hydrogel is a bit restricted recently owing to some limitations, such as transporting the therapeutic agent more accurately to the target site and prolonging their retention locally. This review focuses on the advances in injectable hydrogels for CVD, detailing the types of injectable hydrogels (natural or synthetic), especially that complexed with stem cells, cytokines, nano-chemical particles, exosomes, genetic material including DNA or RNA, etc. Moreover, we summarized the mainly prominent mechanism, based on which injectable hydrogel present excellent treating effect of cardiovascular repair. All in all, it is hopefully that injectable hydrogel-based nanocomposites would be a potential candidate through cardiac repair in CVDs treatment.

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Human placenta hydrogel reduces scarring in a rat model of cardiac ischemia and enhances cardiomyocyte and stem cell cultures

Cited by 57 publications

References 34 publications

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

Injectable Hydrogel-Based Nanocomposites for Cardiovascular Diseases

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Human placenta hydrogel reduces scarring in a rat model of cardiac ischemia and enhances cardiomyocyte and stem cell cultures

Cited by 57 publications

References 34 publications

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Extracellular Matrix Materials for Cardiac Repair and Regeneration

Decellularized Aortic Scaffold Alleviates H2O2-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis

Injectable Hydrogel-Based Nanocomposites for Cardiovascular Diseases

Contact Info

Product

Resources

About

Decellularized Aortic Scaffold Alleviates H₂O₂-Induced Inflammation and Apoptosis in CD34+ Progenitor Cells While Driving Neovasculogenesis