Human papillomavirus infection of the uterine cervix: histological appearances in 28 cases identified by immunohistochemical techniques.

Dyson, J L; Walker, Patrick; A, Singer

doi:10.1136/jcp.37.2.126

Cited by 41 publications

(8 citation statements)

References 12 publications

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“…The histological appearances fell into five categories, comprising cervical intraepithelial neoplasia grade 2 or 3 as defined by Buckley et al'2 with or without evidence of viral infection (koilocytosis) as defined by Dyson et al,13 genomic DNA extra bands were seen (indicated with arrowheads in fig 2c, e), which probably represent the junctions between cellular and viral DNA. We cannot, however, rule out the presence of oligomeric viral genomes.…”

Section: Resultsmentioning

confidence: 99%

DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.

Millan¹,

Davis²,

Torbet³

et al. 1986

BMJ

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Punch biopsy specimens of the cervix were examined both histologically and for the presence of human papillomavirus (HPV) DNA sequences. The presence of HPV DNA sequences was sought with the Southern blot technique using radioactively labelled HPV-6, 11, 16, and 18 DNA probes, both together and separately. Twenty six biopsy specimens were examined. Histological examination showed cervical intraepithelial neoplasia grade 2 or 3 in 16 specimens, viral changes (koilocytosis) in four, and inflammation or a normal appearance in three. Eleven specimens were negative for HPV DNA sequences, 10 contained HPV-16 DNA, four contained HPV-18 DNA, and one contained both HPV-18 and HPV-11 DNA. Episomal HPV-16 DNA was detected in one case of cervical intraepithelial neoplasia grade 3 and in five cases of cervical intraepithelial neoplasia grade 2/3 with koilocytosis; and episomal HPV-18 DNA was found in two specimens classed as cervical intraepithelial neoplasia grade 2/3, one of which also contained HPV-11 DNA, and in one specimen that showed viral changes alone. Integrated HPV DNA was found in six specimens (four with HPV-16 DNA and two with HPV-18 DNA), including two cases of chronically inflamed cervix with no histological evidence of viral infection or cervical intraepithelial neoplasia.Detection ofviral DNA in early lesions may identify patients at risk of malignant progression. This is the first report of HPV-18 DNA in cervical intraepithelial neoplasia in Scotland.

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Section: Resultsmentioning

confidence: 99%

DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.

Millan¹,

Davis²,

Torbet³

et al. 1986

BMJ

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“…Koilocytes were recognized by the presence of a perinuclear halo and an irregular hyperchromatic nucleus that was usually either curved or elongated. Pure koilocytosis was distinguished from CIN by the presence of a single basal cell layer and a normal nucleo-cytoplasmatic ratio in the deeper layers of the epithelium (8,19,31).…”

Section: Methodsmentioning

confidence: 99%

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CINI

Iversen¹,

Duun

Sebbelov³

et al. 1992

APMIS

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The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CIN I. APMIS 100: 280-286, 1992. Thirthy-one patients with koilocytosis and/or concomitant CIN I were analysed for the presence of HPV types 11, 16 and 18 by in situ hybridization and Southern blot analysis. The prevalence of HPV was 48%) and 55%, respectively, when measured by the two methods and among the HPV positive, HPV 11 and 16 were present in 47% and 60%, respectively, whereas HPV 18 was not found.Clinical epidemiological studies support the hypothesis that precancerous and cancerous lesions of the uterine cervix are related to human papillomavirus (HPV) infection (1,9,16,17, 33, 34). Distinct subtypes of HPV have been demonstrated in 60-100% of precancerous and malignant lesions of the cervix, and an essential event in the malignant transformation may be the integration of the viral genome into the chromosomal DNA (7,24,27, 32). More than 60 HPV types and subtypes have been cloned and characterized (6). At present more than 20 different HPV types have been associated with infections in the genital mucosa. HPV 6 and 11 are most frequently found in genital condylomas and in lower grade cervical intraepithelial neoplasia (CIN I) (reviewed by Bosch & Munoz (1). The HPV types 16, 18, 31, 33, 35, 45, 51, and 52 are most often associated with higher grades of dysplasia (CIN I1 and CIN III/CIS) and invasive carcinoma of the uterine cervix (1). The prevailing types are HPV 16 and 18, which can be demonstrated in 60-100% of specimens with CIN 11, 111 and invasive cancer (1, 32). Typing of HPV in early lesions is believed to be of prognostic value and allows identification of possible risk groups. It is therefore desirable to develop diagnostic methods which are reliable, fast and inexpensive. Genital lesions are detected by clinical examination and by analysis of histological and cytological specimens, but identification of HPV types is only possible by nucleic acid hybridization or polymerase chain reaction (PCR) (14,18, 32).Brigati, D. J., Myerson, D. & Leary, J. J.: Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. J. Virol. 126: [32][33][34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50] 1983. Campion, M. J., McCance, D. J., Cuzick, J. & Singer, A.; Progressive potential of mild cervical atypia: prospective cytological, colposcopic, and virological study. Lancet Aug 2: 237-240, 1986. Caussy , D., Marrett, L. D., Worth, A. J., McBride, M. & Rawls, W E.: Human papillomavirus and cervical intraepithelial neoplasia in women who subsequently had invasive cancer. Can. Med. As-Collins, J. E., Jenkins, D. & McCance, D. J.: Detection of human papillomavirus DNA sequences by in situ DNA-DNA hybridization in cervical intraepithelial neoplasia and invasive carcinoma: a retrospective study. J. Clin. Pathol. DeVilliers, E. M.: Heterogeneity of the human 135-151, 1989. SOC.

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“…Due to its mutagenic activity (Schlehofer and zur Hausen, 1982), HSV could act as an initiating agent . Recently, attention has been focused upon HPVs: their association with genital tumors was suggested by serological (Baird, 1983), histopathological (Fu et al, 1983;Pilotti et al, 1982) and immunohistochemical studies (Pilotti et al, 1984;Dyson et al, 1984). HPV-16 and HPV-18 DNA sequences have consistently been detected in biopsies from female genital neoplasms both by Southern blot analysis (Durst et al, 1983;Boshart et al, 1984: Scholl et al, 1985Fukushima et al, 1985; Di pothesis, HPV early viral functions, which are normally suppressed by trans-acting cellular regulatory factors, may induce cell transformation once released from the host cell control mechanisms.…”

mentioning

confidence: 99%

Simultaneous presence of herpes simplex and human papilloma virus sequences in human genital tumors

Luca¹,

Rotola²,

Pilotti

et al. 1987

Intl Journal of Cancer

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Herpes simplex virus (HSV) and human papillomavirus (HPV) sequences were analyzed in tumors of the female lower genital tract, by probing DNA from 13 intraepithelial and 30 invasive neoplastic lesions with radiolabelled HPV-16 and HPV-18 DNA as well as cloned fragments of HSV-2 DNA. Careful removal of stromal tissue from the pathological specimens allowed authentic tumor DNA to be processed. Normal genital tissue obtained from the patients and genital condylomata were included as internal controls. The presence of HPV-16 or 18 DNA was detected in 12/13 (92.3%) intraepithelial neoplasms and in 16/30 (53.3%) invasive carcinomas. No significant difference was detected in titer or frequency of antibodies to HPV group-specific antigen in sera from patients and controls. Hybridization to BgIII N fragment of HSV-2 DNA was detected in 4/13 (30.8%) intraepithelial neoplasms and 4/30 (13.3%) invasive carcinomas but in none of the control tissues. All the 8 samples harboring HSV-2 homologous sequences were also positive for HPV, supporting the hypothesis of a synergistic association between the 2 viruses. The hybridization analyses performed to study c-myc involvement in genital oncogenesis did not reveal c-myc amplification in either invasive or pre-invasive lesions.

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Human papillomavirus infection of the uterine cervix: histological appearances in 28 cases identified by immunohistochemical techniques.

Cited by 41 publications

References 12 publications

DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.

DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.

The prevalence of human papillomavirus in cervical lesions with koilocytosis and/or CINI

Simultaneous presence of herpes simplex and human papilloma virus sequences in human genital tumors

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