DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.

Millan, David; Davis, Judith A.; Torbet, T. E.; Campo, M. Saveria

doi:10.1136/bmj.293.6539.93

Cited by 30 publications

(9 citation statements)

References 15 publications

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“…This study confirmed the previous results [1][2][3][4][5][6][7], which reported that cervical HPV infection is an important risk factor for cervical dysplasia, frequently associated with neoplastic degeneration. A significantly higher percentage of HPV DNA positivity was observed in women with mild cervical dysplasia than in controls, suggesting that HPV is an important agent in the development of cervical neoplasia.…”

Section: Discussionsupporting

confidence: 92%

“…Human papillomavirus (HPV) has been associated with the development of cervical carcinoma and its pre cursor lesions [1][2][3][4], Several studies have revealed HPV DNA types 16 and 18 in high-grade squamous intraepi thelial lesions and invasive carcinomas [5][6][7]; however, studies of the prevalence of HPV DNA infection found that a significant proportion of women positive for HPV DNA had reversible low-grade lesions or no clinical and cytologic evidence of cervical disease [8][9][10]. As these studies were primarily cross-sectional and observational, the long-term implication of HPV DNA positivity re mains undetermined as well as the cofactors conditioning the natural history of HPV-DNA-associated lesions, which are still unclear.…”

Section: Introductionmentioning

confidence: 99%

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HPV DNA Positivity and Natural Killer Cell Activity in the Clinical Outcome of Mild Cervical Dysplasia: Integration between Virus and Immune System

Garzetti

Ciavattini²,

Goteri³

et al. 1995

Gynecol Obstet Invest

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The objective was to examine the prevalence of human papillomavirus (HPV) DNA infection in mild cervical dysplasia and to evaluate longitudinally the persistence of HPV DNA positivity in an observational study, aiming at identifying the role of peripheral blood lymphocyte natural killer activity in the natural history of dysplastic disease. Twenty-three patients with histologically proven mild cervical dysplasia were selected. The HPV DNA positivity, determined by polymerase chain reaction, and cervical dysplasia were monitored cytologically and colposcopically at the 3rd (time 1), 6th (time 2) and 12th months (time 3), and defined by biopsies for routine histology taken at times 2 and 3. For each patient included in the study, the immune reactivity was evaluated at the time of diagnosis and afterwards, longitudinally during the follow-up. The immune status analysis included T lymphocyte subsets (CD3, CD4, CD8, CD56, CD 16 monoclonal antibodies by Beckton Dickinson, Mountain View, Calif., USA) and determinations of natural killer cell activity (against the sensitive cell line K 562). Eighteen out of the 23 women with mild cervical dysplasia (78.3%) were found positive for HPV DNA, with a significantly high representation of HPV DNA type 16 (55.6% of cases). At the end of the study, 12 out of 18 HPV-DNA-positive women became negative (defined by two or more negative tests) for the original HPV DNA type, with 66.7% of spontaneous HPV DNA negativization rate (p = 0.6). In 83.3% of the women with definitive HPV DNA negativization and in 7.5% of those with a constant HPV DNA negativity, the resolution of HPV DNA infection was associated with a clinical-pathologic remission of the lesion. Patients with spontaneous HPV DNA negativization and/or clinical-pathologic resolution of cervical dysplasia had a significantly higher mean value of natural killer activity than those with persistent disease. No significant modifications were observed with respect to lymphocyte subsets. The longitudinal evaluation of natural killer activity showed constantly low values of natural cytotoxicity in patients with persistent dysplasia, while women with regressive lesions had a significant increase in immune reactivity during the follow-up. The majority of patients with mild HPV-DNA-associated dysplasia can be followed with confidence and with the expectation that the minimal degree of dysplasia will eventually disappear. The evaluation of natural cytotoxicity is a useful parameter of functional immune status, correlated with the natural history of the disease.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

HPV DNA Positivity and Natural Killer Cell Activity in the Clinical Outcome of Mild Cervical Dysplasia: Integration between Virus and Immune System

Garzetti

Ciavattini²,

Goteri³

et al. 1995

Gynecol Obstet Invest

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“…1, C1, lane 1). Different polymorphism of PstI restriction sites of HPV-18 has previously been reported in a cervical carcinoma biopsy in the west of Scotland (Millan et al 1986), indicating a degree of heterogeneity of this virus in the area.…”

confidence: 69%

Human Papillomavirus DNA in Biopsies of Oral Tissues

Yeudall

Campo

1991

Journal of General Virology

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“…However some samples showed deletions or certain genomic rearrangements leading to an atypical pattern which also may be indicative of integration of HPV-16 DNA into the host cell genome (Lehn et al, 1985;Millan et al, 1986;Choo et al, 1987;Takebe et al, 1987;Wilczynski et al, 1988). In almost all cases, two PstI fragments, i.e.…”

Section: Discussionmentioning

confidence: 99%

Analysis by polymerase chain reaction of the physical state of human papillomavirus type 16 DNA in cervical preneoplastic and neoplastic lesions

Das

Sharma

Gopalakrishna

et al. 1992

Journal of General Virology

142

120

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Integration of human papillomavirus (HPV) DNA into the host cell genome is believed to be essential for malignant progression. However unambiguous detection of the physical state of HPV is a difficult and timeconsuming procedure. To resolve this issue a simple, rapid and highly sensitive technique of polymerase chain reaction (PCR) has been utilized for detecting the physical state of HPV-16 DNA. Investigations were carried out in 122 cervical specimens comprising the whole spectrum of cervical lesions starting from cervical dysplasia to invasive carcinoma including HPV-16-positive normal controls. A pair of oligonucleotide primers specific to the E2 open reading frame, which is often deleted or disrupted following HPV integration, was used for the study. Distinction between episomal and integrated forms of viral DNA was accomplished by detecting amplification of the E2-specific fragment (1139 bp) in the PCR product. The PCR results were compared with those obtained by the conventional methods of Southern blotting, twodimensional gel electrophoresis and chromosomal in situ hybridization; a high degree of agreement was observed between the methods. The findings indicate that although integrated forms of HPV-16 DNA were detected in more than 70% of cervical cancer specimens, integration was less frequent (23%) in severe dysplasia and carcinoma in situ. Only 2.5 % of cases showed both episomal and integrated forms ofHPV-16 DNA. The difference between episomal and integrated forms was statistically significant (P<0.01). The absence of integration in about 30% of cancer cases suggests that integration of HPV may not be necessary for malignant progression and alternative mechanism(s) of malignant transformation may occur without HPV integration. The PCR test thus provides an effective complement to Southern blotting and twodimensional gel electrophoresis for accurate detection of the integration of HPV DNA.

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DNA sequences of human papillomavirus types 11, 16, and 18 in lesions of the uterine cervix in the west of Scotland.

Cited by 30 publications

References 15 publications

HPV DNA Positivity and Natural Killer Cell Activity in the Clinical Outcome of Mild Cervical Dysplasia: Integration between Virus and Immune System

HPV DNA Positivity and Natural Killer Cell Activity in the Clinical Outcome of Mild Cervical Dysplasia: Integration between Virus and Immune System

Human Papillomavirus DNA in Biopsies of Oral Tissues

Analysis by polymerase chain reaction of the physical state of human papillomavirus type 16 DNA in cervical preneoplastic and neoplastic lesions

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