Human Papillomavirus DNA in Biopsies of Oral Tissues

Yeudall, W. Andrew; Campo, M. Saveria

doi:10.1099/0022-1317-72-1-173

Cited by 88 publications

(39 citation statements)

References 22 publications

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“…This product may be indicative of a low copy number of another closely related HPV type and this is currently being investigated. Other HPV types which have been reported from the oral cavity such as HPV6, HPV-11 (Loning et al, 1985) and HPV-4 (Yeudall & Campo, 1991) are also undetectable in lines SCC-4, SCC-9, SCC-12B, SCC-12F, SCC-13, SCC-15 and SCC-25 (M. Stanley-personal communication; A. Yeudall-personal communication).…”

Section: Primersmentioning

confidence: 98%

“…The primers for the amplification of HPV-16 and HPV-18 have been described previously (Yeudall & Campo, 1991) and amplify fragments of the E6/E7 (HPV-16) or E6 regions (HPV-18) which are 165 and 99bp respectively.…”

Section: Primersmentioning

confidence: 99%

“…This is particularly relevant to oral SCC since HPV16 and 18 are known to occur in the oral cavity (Maitland et al, 1987;1989;Yeudall & Campo, 1991) and can immortalise oral keratinocytes (Park et al, 1991). Fifteen of the SCC lines under investigation (including all the negatively-staining ones) were repeatedly screened for HPV-16 and 18 DNA by PCR and were found to be negative.…”

Section: Primersmentioning

confidence: 99%

“…The last two viruses are of particular significance to our study since they have been reported to occur in both normal and malignant tissue of the oral cavity (Maitland et al, 1987;1989, Yeudall & Campo, 1991. In addition, transfection of these viruses in vitro into human keratinocytes from both the epidermis (Pirisi et al, 1987;Kaur & McDougall, 1988) and the oral cavity (Park et al, 1991) leads to their immortalisation, and both the E6 and E7 proteins are important for this process (Hawley-Nelson et al, 1989;Munger et al, 1989).…”

mentioning

confidence: 90%

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Gene mutations and increased levels of p53 protein in human squamous cell carcinomas and their cell lines

Burns

Baird²,

Clark³

et al. 1993

Br J Cancer

118

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(Mitsudomi et al., 1992). In 11/13 cases only the mutant alleles were expressed suggesting loss or reduced expression of the wild type alleles in these cases. Six of the mutations were also detected in the SCCs from which the lines were derived, strongly suggesting that the mutations occurred, and were selected, in vivo.The 12th mutation GTG-*GGG (valine-*glycine) at codon 216 was expressed in line SCC-12 clone B along with an apparently normal p53 allele and is to our knowledge a novel mutation. Line BICR-19 also expressed a normal p53 allele in addition to one where exon 10 was deleted.Additionally 15 of the SCC lines (including all of those which did not show elevated p53 protein levels) were screened for the presence of human papillomavirus types 16 and 18 and were found to be negative.These results are discussed in relation to the pathogenesis of SCC and the immortalisation of human keratinocytes in vitro.

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Section: Primersmentioning

confidence: 98%

Section: Primersmentioning

confidence: 99%

Section: Primersmentioning

confidence: 99%

mentioning

confidence: 90%

See 2 more Smart Citations

Gene mutations and increased levels of p53 protein in human squamous cell carcinomas and their cell lines

Burns

Baird²,

Clark³

et al. 1993

Br J Cancer

118

View full text Add to dashboard Cite

show abstract

“…Several methods can be used to detect HPV and different techniques with varying sensitivity and specificity could potentially influence the detection rate of HPV. In situ hybridization, immunohistochemistry and Southern blotting were utilized in the early period of HPV detection and were less sensitive than PCR (Yeudall & Campo, 1991;Frazer et al, 1993;Kay et al, 2002;Boy et al, 2006). In our study, quantitative PCR was used for the detection of HPV which is sensitive and could detect as low as 9 viral copies per reaction.…”

Section: Enumeration Of Viral Copy Number By Real-time Pcrmentioning

confidence: 99%