Human papillomavirus infection in women attended at a cervical cancer screening service in Natal, Brazil

Abstract

We analyzed cervical specimens of 202 women, aged 15 to 64 years, attended at Luis Antonio Hospital, Natal, Brazil, to determine the prevalence of HPV and identify the more frequent genotypes and risk factors for HPV infection in women attended at a cervical cancer screening service. Two specimens were collected from each patient: one for cytological examination and the other to detect HPV DNA by PCR, and typing by dot blot hybridization. A total of 54.5% of the sample had normal cytology and 45.5% had cytological alterations. HPV was detected in 24.5% of the cytologically normal women and in 59.8% of those with altered cytology. Both single and double HPV infection increased the likelihood of cytological alterations. Thirteen types of HPV were identified, most of which were high risk. HPV 16 was the most prevalent single-type infection, followed by HPV 58. The most frequent double infection was the association between HPV 56 and 57. The prevalence of HPV in cytologically normal women was greater than that reported for countries on all the continents except Africa. The inverse was observed in women with cytological alterations. The distribution of HPV types was similar to that described for the Americas, with some differences. Multiple sexual partners was the only risk factor showing an association with the presence of HPV infection.

Human papillomavirus infection in women from Tlaxcala, Mexico

“…Concurrent infection with more than one HPV genotype was found in 10.8% of the analyzed population. This value is in the range observed for multiple infections among different studies (4,8,12,20,22).…”

Distribuição dos genótipos de papilomavírus humano em mulheres do estado da Bahia, Brasil

“…Os estudos no Brasil 15,16 apontam para a maior prevalência do HPV 58, principalmente na região Nordeste do país 10 . Porém, no presente estudo, o HPV 58 foi o oitavo genótipo mais comum.…”

“…Os achados cito-histopatológicos foram classificados em grupos de: achados negativos para neoplasia (exames citopatológico e histopatológico negativos), lesão de baixo grau (achado citopatológico -lesão intraepitelial de baixo grau -ou achado histopatológico -neoplasia intraepitelial cervical grau 1, neoplasia intraepitelial vaginal grau 1 ou condiloma e lesão de alto grau (achado citopatológico -lesão intraepitelial de alto grau -ou histopatologia com laudo maior ou igual a neoplasia intraepitelial cervical grau 2 ou neoplasia intraepitelial vaginal grau 2). RESULTADOS: O genótipo de alto risco mais frequente foi o HPV 16, com 18,5%; intervalo de confiança de 95% (IC95%) 14,6-23,0, seguido pelo HPV 56 (14%; IC95% 10,5-18,0) e o HPV 39 (13,4%; IC95% 9,(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)8). O HPV 18 (5,4%; IC95% 3,3-8,3) esteve entre os menos comuns.…”

“…Os resultados sobre a distribuição dos genótipos de HPV no Brasil são mais modestos, e os estudos são concentrados nos estados da região Sudeste 9 . Em concordância com as observações mundiais, o HPV 16 foi o tipo mais frequente em pesquisas realizadas em diferentes regiões do país [10][11][12][13][14][15][16][17][18] . Entretanto, a heterogeneidade na distribuição é encontrada principalmente em relação aos demais tipos [5][6][7][8]19,20 .…”

Prevalence and genotypic diversity of cervical human papillomavirus infection among women from an urban center in Brazil

“…The worldwide prevalence in women with normal cytology is approximately 10% (de Sanjosé et al, 2007), whereas in women with abnormal cytology it is approximately 35% (Molijn et al, 2005). Studies conducted in several cities and states revealed a prevalence of HPV infection between 24.8 and 35.0% in Brazil (Nonnenmacher et al, 2002;Fernandes et al, 2008;Krambeck et al, 2008;Lippmann et al, 2010;Oliveira et al, 2010).…”