Human papillomavirus identified by nucleic acid hybridization in concomitant nasal and genital papillomas

Abstract

Presence of human papillomavirus (HPV) as the etiologic agent in nearly all upper respiratory tract recurrent papillomas is well-established. The technique of nucleic acid hybridization now allows specific typing of HPV with a high degree of accuracy. This article reports a series of nine consecutive patients treated for nasal papillomas over the past 9 years. Eight of these patients had a personal history of genital papillomas (seven patients) or exposure (one patient). With the use of in situ hybridization and autoradiographic technique on paraffin-embedded tissue sections, HPV RNA type 6/11 was expressed in eight of nine nasal papillomas, and corresponding HPV types were also found in the two cases with which concurrent anogenital papilloma tissue was also available for analysis. Human papillomavirus RNA types 16 and 18 were not detected in any of the specimens. Signals of HPV messenger RNA type 6/11 were stronger in the fungiform areas than in the inverted areas of papillomas.

Significance of human papillomavirus in sinonasal papillomas

“…However, the viral aetiology could be a possible explanation for multicentric disease in some patients, resulting in multiple recurrences in spite of a radical resection. 17 As only low-risk HPV types were detected in our series and no correlation was found to malignancy and recurrence of disease, screening for HPV seems not to be useful as a prognostic parameter.…”

A high prevalence of human papillomavirus DNA in recurrent nasal papillomas

“…Among them, all of seven recurrent cases contained HPV DNA. Klemi et al [7] also reported that all four cases of recurrent sinonasal papillomas yielded HPV DNA types 11/16, and Fu et al [12] reported all three recurrent fungiform nasal papillomas studied had HPV DNA types 6/11. Conversely, HPV was not detected in normal nasal mucosa [13].…”

“…Among them, four (SO.OO/) of the five recurrent cases (cases 1-5) were HPV DNA-positive whereas only one (14.3%) of seven non-recurrent cases (cases [6][7][8][9][10][11][12] was HPV DNA-positive. The detection rate of HPV DNA in recurrent cases analysed by Fisher's exact probability test was significantly higher than that in non-recurrent cases (p = 0.045).…”

“…A clinical tendency for the lesion to recur even after adequate removal suggests the possibility of papillomas being induced by HPV Laryngeal papillomas are classified clinically into four groups: juvenile multiple, juvenile single, adult multiple and adult single [22]. HPV type 6/11 has been detected in almost 100% of juvenile and adult multiple laryngeal papillomas, strongly suggesting a viral aetiology for these benign tumors [23-251. Conversely, the reported prevalence of HPV in fungiform exophytic and inverted nasal papillomas has varied [l, [3][4][5][6][7][8][9][10][11][12][13]151. The methods and techniques used for detection of HPV as well as the patients' geographic location seem to influence the results.…”

A possible role for human papillomaviruses in head and neck cancer

“…Focal epithelial hyperplasia is often associated with HPV-13 [50] and HPV-32 [51], which are not found in other lesions. Exophytic nasal papillomas also usually contain HPV-6 or HPV-11 [52][53][54][55][56][57], although HPV-57b has been identified in both exophytic and inverted papillomas of the nasal cavity [58]. Most exophytic papillomas are HPV positive (70 to 75%), while the reported frequency of HPV DNA detection in inverting papillomas ranges from 0% to 89%.…”