Human papillomavirus (HPV) types 2 and 57 in oral verrucae demonstrated by in situ hybridization

Abstract

Twenty-one cases of verrucae vulgaris (oral warts) were investigated for human papillomavirus (HPV)-group specific antigen by immunocytochemistry and for HPV types 1, 2, 4, 6, 11, 16, 18 and 57 by DNA in situ hybridization with biotinylated probes. Twelve (57%) cases demonstrated the presence of HPV-group specific antigen. Fifteen (71%) cases showed the presence of HPV DNA, 13 of which (87%) demonstrated both HPV types 2 and 57 in the same cells and 2 of which (13%) demonstrated only HPV 2. Six cases were negative for HPV 2 and 57 and all 21 cases (100%) were negative for HPV types 1, 4, 6, 11, 16 and 18. Results indicate the association of a new and as yet unidentified HPV type, closely related to HPV 2 and 57, with oral warts. The identification of both cutaneous type HPV 2 and another type closely related to HPV 2 and 57 in oral verrucae on keratinized and non-keratinized mucosal surfaces indicates the possibility of a latent infection; three patients had a history of warts on their hands, suggesting autoinoculation. This study indicated that future investigations of oral warts, based on a correlation of clinical and histological features with HPV types by DNA in situ hybridization, are called for.

Recurrent Oral Squamous Papilloma in a HIV Infected Patient: Case Report

“…This infection often occurs on oral mucosa and the sites most involved are labial mucosa, with 55% of cases, the palate, jugal mucosa, gums, tonsils, uvula and roof of the mouth (9). Although the tongue is not commonly affected, the case presented both lesions on the tip of this organ.…”

“…(21) Owing to the oncogenic role of certain types of HPV, the relation between this virus and carcinogenesis in oral mucosa is still controversial. Various studies have identified HPV in biopsies of oral SCC and VC (4,9,12). Among HPV types found in the oral mucosa, types 6 and 11 are more prevalent in benign lesions on the oral epithelium and types 16 and 18 more prevalent in malignant lesions.…”

Correlação da infecção viral pelo papilomavírus humano com as lesões papilomatosas e o carcinoma epidermóide na boca e orofaringe

“…Quanto à distribuição histológica da casuística e à presença do HPV, verificamos (Tabela 3) o predomínio nos papilomas (100%), seguido das hiperplasias (95%) e finalmente pelos carcinomas (44,7%), que talvez possa ser explicado pelo aumento de práticas sexuais orais, e, em se tratando de lesões benignas (papilomas e hiperplasias), prevalecem em adolescentes e adultos jovens 12,13 . Ao lado disso, próteses mal-adaptadas podem determinar o sangramento de hiperplasias, apesar de sua menor freqüência quando desenvolvida a partir do trauma mecânico 11 .…”

“…Quanto à localização, houve o predomínio da língua (48,5%) em relação às demais, sendo o carcinoma epidermóide prevalente em 57,6% sobre a hiperplasia (30,3%) e o papiloma (12,1%) (Tabela 1). 12 . Nestes estudos, assim como no nosso, não houve diferenciação entre os papilomas e os condilomas ou as verrugas orais.…”

Prevalência do papilomavírus humano (HPV) na cavidade oral e na orofaringe

“…They observed a variation in the HPV detection rates from 43% to 100% in oral warts 29,31,32 . HPV prevalence in oral warts seems to be HPV 2, followed by the HPV 57, although more studies are necessary to identify the broad spectrum oral infectiosn caused by skin HPV types 20,33 .…”