Human papillomavirus (HPV) DNA in focal epithelial hyperplasia by in situ hybridization

Abstract

Eighteen cases of focal epithelial hyperplasia (FEH) were investigated for the presence of human papillomavirus (HPV) group specific antigen by immunocytochemistry and HPV types 1, 6, 11, 13, 16, 18 and 32 by DNA in situ hybridization employing biotinylated probes. Seven (39%) specimens demonstrated the presence of HPV group specific antigen. Fifteen (83%) specimens were positive for HPV DNA: 9 (60%) showed HPV 32, of which 6 were on non-keratinized mucosa and 3 on border of keratinized and non-keratinized mucosa; 5 (33%) showed HPV 13, 4 lesions on keratinized mucosa and 1 on non-keratinized mucosa; 1 (7%) specimen on non-keratinized mucosa showed HPV-11 related. Two specimens on different sites from one patient showed the same HPV type and one patient had, in addition to FEH, a squamous papilloma also demonstrating the same HPV type. Results show a specific HPV distribution pattern in the epithelium indicating areas of high viral concentration adjacent to areas of low or no viral concentration. This study also indicates the possibility of tissue-site specificity or a latent infection and the possibility of a yet unidentified HPV type associated with FEH. It is suggested that future monitoring of patients be carried out with special reference to HPV type and anatomical distribution pattern for FEH lesions.

Prevalência do papilomavírus humano (HPV) na cavidade oral e na orofaringe

“…It is rarely observed in caucasians 35 . It has also been described in Israel, South Africa and Sweeden 36 .…”

“…HPV 13 and 32 were considered specific in focal epithelial hyperplasia, however, HPV 32 was also found in other oral lesions, but never outside the oral cavity 36,39,40 .…”

Hyperplasie épithéliale focale et syndrome de Waldmann. Présentation d’un cas

“…Sa prévalence exacte n'est pas connue puisque la plupart des publications rapportent des cas isolés et seulement quelques publications comportent un grand nombre de patients [7,8]. L'HEF est due à des VPH de types 13 ou 32 [9,10]. Elle se traduit par de multiples papules ou nodules indolores, de nombre et de taille variables, mesurant de 0.1 à 1 cm, pouvant intéresser l'ensemble de la muqueuse buccale, mais le plus souvent uniquement les lèvres et les joues.…”

Hiperplasia Epitelial Focal: Uma Revisão De Literatura Narrativa

“…O Papiloma vírus Humano (HPV) tem sido apontado como um fator etiológico desta proliferação benigna assintomática da mucosa bucal, principalmente o genótipo 32 (HPV 32) em pessoas de mais idade, e o genótipo 13 (HPV 13), que parece estar igualmente envolvido no desenvolvimento da doença tanto em pacientes jovens quanto nas idades mais avançadas (PADAYACHEE; VAN WYK, 1991;JABLONSKA, 2000). Ainda, têm também sido envolvidos fatores como condições socio-econômicas e genéticos (CARLOS; SEDANO, 1994;SAID et al, 2012).…”