Human papillomavirus genotypes distribution in 175 invasive cervical cancer cases from Brazil

Abstract

BackgroundInvasive cervical cancer is the second most common malignant tumor affecting Brazilian women. Knowledge on Human Papillomavirus (HPV) genotypes in invasive cervical cancer cases is crucial to guide the introduction and further evaluate the impact of new preventive strategies based on HPV. We aimed to provide updated comprehensive data about the HPV types’ distribution in patients with invasive cervical cancer.MethodsFresh tumor tissue samples of histologically confirmed invasive cervical cancer were collected from 175 women attending two cancer reference hospitals from São Paulo State: ICESP and Hospital de Câncer de Barretos. HPV detection and genotyping were performed by the Linear Array HPV Genotyping Test (Roche Molecular Diagnostics, Pleasanton,USA).Results170 out of 172 valid samples (99%) were HPV DNA positive. The most frequent types were HPV16 (77.6%), HPV18 (12.3%), HPV31 (8.8%), HPV33 (7.1%) and HPV35 (5.9%). Most infections (75%) were caused by individual HPV types. Women with adenocarcinoma were not younger than those with squamous cell carcinoma, as well, as women infected with HPV33 were older than those infected by other HPV types. Some differences between results obtained in the two hospitals were observed: higher overall prevalence of HPV16, absence of single infection by HPV31 and HPV45 was verified in HC-Barretos in comparison to ICESP patients.ConclusionsTo our knowledge, this is one of the largest studies made with fresh tumor tissues of invasive cervical cancer cases in Brazil. This study depicted a distinct HPV genotype distribution between two centers that may reflect the local epidemiology of HPV transmission among these populations. Due to the impact of these findings on cervical cancer preventive strategies, extension of this investigation to routine screening populations is warranted.

Potential impact of a 9-valent HPV vaccine in HPV-related cervical disease in 4 emerging countries (Brazil, Mexico, India and China)

“…Our data supported HPV 16 as the most common type in ICC, being HPV18 consistently the second most detected type in the four countries [9,26,30,31]. Although HPV16 was the most commonly detected type in HPV-positive women with precancerous cervical lesions, HPV18 and 45 were underrepresented compared to the high contribution in ICC, and other HPV types gained prominence but varied by country.…”

“…Similar to other countries in East Asia, HPVs 52 and 58 had a prominent role in China where these types were identified in more than 7% of cases [9,24,25]. HPV 45 was more common in Brazil and Mexico (more than 6% of cases) and HPV33 accounted for more than 5% of cases in Brazil [9,26,27]. Finally, a wide variety of HPV types were observed in India with HPV 33 being slightly more prominent than others [28,29].…”

Expression analysis of transglutaminase 2 in premalignant lesions of the cervix

“…The presence of HPV in normal samples is also shown in other studies [2,22,23]. In a study performed in the city of Porto Alegre, the prevalence of HPV was demonstrated in 11.6% of women without LSIL or HSIL [25].…”

“…al. demonstrated that the most frequent types were HPV16 (77.6%), HPV18 (12.3%), HPV31 (8.8%), HPV33 (7.1%) and HPV35 (5.9%) [23]. According to other studies, HPV16 is the most prevalent type in all regions of Brazil.…”

Human Papillomavirus Genotype Distribution among Cervical Cancer Patients prior to Brazilian National HPV Immunization Program

“…), was also observed in another Southeastern State, São Paulo [19], where 17 different HPV types were identified by Linear Array HPV Genotyping Test (LA, Roche Molecular Diagnostic). Comparisons with other Brazilian data from cervical cancer samples are limited due to either the techniques used for HPV identification (which only allowed identification of a few HPV types other than HPV16 and HPV18) or the small number of samples analyzed [20–24].…”