Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening

Rymsza, Taciana; Ribeiro, Eliane Aline; Carvalho, Luis Felipe das Chagas e Silva de; Bhattacharjee, Tanmoy; Canevari, Renata de Azevedo

doi:10.1016/j.saa.2018.02.004

Cited by 17 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The greatest benefit of FTIR technique lies in its highly sensitive acquisition of the whole ‘-omics’ of a biological sample, which enables the detection of molecular changes that may reflect the onset and progression of a disease, thus enabling disease diagnosis and monitoring [ 29 ]. Up to now, this technique has been widely used in the medical field for cancer diagnoses, such as skin [ 30 ], breast [ 31 , 32 ], cervical [ 33 ], colon [ 34 ] and kidney tumors [ 35 ], detections of antibiotics’ resistant Escherichia coli bacteria [ 36 ], human papillomavirus [ 37 ] and malaria infected red blood cells [ 38 ], and monitoring of tendinopathy [ 39 ] and chronic venous leg ulcer exudates [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

et al. 2019

View full text Add to dashboard Cite

It is difficult to determinate the cause of death from exposure to fatal hypothermia and hyperthermia in forensic casework. Here, we present a state-of-the-art study that employs Fourier-transform infrared (FTIR) spectroscopy to investigate the hypothalamus tissues of fatal hypothermic, fatal hyperthermic and normothermic rats to determine forensically significant biomarkers related to fatal hypothermia and hyperthermia. Our results revealed that the spectral variations in the lipid, protein, carbohydrate and nucleic acid components are highly different for hypothalamuses after exposure to fatal hypothermic, fatal hyperthermic and normothermic conditions. In comparison with the normothermia group, the fatal hypothermia and hyperthermia groups contained higher total lipid amounts but were lower in unsaturated lipids. Additionally, their cell membranes were found to have less motional freedom. Among these three groups, the fatal hyperthermia group contained the lowest total proteins and carbohydrates and the highest aggregated and dysfunctional proteins, while the fatal hypothermia group contained the highest level of nucleic acids. In conclusion, this study demonstrates that FTIR spectroscopy has the potential to become a reliable method for the biochemical characterization of fatal hypothermia and hyperthermia hypothalamus tissues, and this could be used as a postmortem diagnostic feature in fatal hypothermia and hyperthermia deaths.

show abstract

Section: Introductionmentioning

confidence: 99%

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

et al. 2019

View full text Add to dashboard Cite

show abstract

“…For example, Viana et al [ 13 ] showed the possibility of distinguishing HPV infected samples from normal samples with 76% efficiency. Interestingly, the study by Rymsza et al [ 14 ] showed that FTIR could not distinguish between samples identified as low/high risk by cytology, but PCR showed that all samples contained HPV, proving that FTIR results were more accurate compared to cytology.…”

Section: Discussionmentioning

confidence: 99%

PCR-RFLP and FTIR-based detection of high-risk human papilloma virus for cervical cancer screening and prevention

Melo

Viana

Pupin

et al. 2021

Biochemistry and Biophysics Reports

Self Cite

View full text Add to dashboard Cite

Background Approximately 70% of cervical carcinoma cases show the presence of high-risk Human Papilloma Virus (HPV), especially HPV-16 and HPV-18, and can be used to stratify high risk patients from low risk and healthy. Currently, molecular biology techniques such as polymerase chain reaction (PCR) are used to identify the presence of virus in patient samples. While the methodology is highly sensitive, it is labor intensive and time-consuming. Alternative techniques, such as vibrational spectroscopy, has been suggested as a possible rapid alternative. Therefore, in this study, we evaluate the efficiency of cervical fluid Fourier Transform Infrared spectroscopy (FTIR) in patient risk stratification informed by PCR. Methods Cervical fluid samples (n = 91) were obtained from patients who have undergone routine Papanicolaou (Pap) test. Viral genome was identified and classified as high/low-risk by PCR-Restriction Fragment Length Polymorphism (PCR-RFLP). FTIR spectra were acquired from samples identified by PCR-RFLP as No-HPV (n = 10), high-risk HPV (n = 7), and low-risk HPV (n = 7). Results Of the 91 samples, was detected the viral genome by PCR in 36 samples. Of these 36 samples, nine samples were identified to contain high-risk HPV (HR-HPV) and nine samples were found to have low-risk HPV (LR-HPV). The FTIR spectra acquired from No-HPV, LR-HPV, and HR-HPV showed differences in 1069, 1437, 1555, 1647, 2840, 2919, and 3287 cm -1 bands. Principal Component Analysis (PCA) showed distinct clusters for No-HPV and HR-HPV and No-HPV and LR-HPV, but there was significant overlap in the clusters of HR-HPV and LR-HPV. PCA-Linear Discriminant Analysis (PC-LDA) after Leave One Out Cross Validation (LOOCV) classified No-HPV from HR-HPV and No-HPV from LR-HPV with 100% efficiency in the 1400-1800 cm -1 spectral range. LOOCV classifications for LR-HPV and HR-HPV from each other were 71 and 75%, respectively, in the 2800-3400 cm -1 spectral range. Conclusions The results highlight the high sensitivity of PCR-RFLP in HPV identification and show that FTIR can classify samples identified as healthy, low, and high-risk samples by PCR-RFLP. General significance We show the possibility of using FTIR for initial cervical cancer risk stratification followed by detailed PCR-RFLP investigations for suspect cases.

show abstract

“…We have previously shown that vaginal cells, self-collected using a simple cotton swab and further self-smeared on a glass slide, can be valid sample for HR-HPV detection with PCR [12]. Lately, various PCR-based techniques have been developed to detect HR-HPV DNA but few allow for low-cost detection [13–18]. The PCR GP5+/6+, which targets the L1 region of the HPV genome, coupled with an enzymatic immunoassay (GP-EIA) has been extensively studied and used in different clinical settings, giving good results [19–21].…”

Section: Introductionmentioning

confidence: 99%

Assessment of a new low-cost, PCR-based strategy for high-risk human papillomavirus DNA detection for cervical cancer prevention

et al. 2019

View full text Add to dashboard Cite

Background HPV test implementation as a primary screening tool has the potential to decrease cervical cancer incidence as shown by several studies around the world. However, in many low-resource settings, the HPV test introduction has been backed down mainly due to its price. In this study, we present a novel low-cost strategy involving simple devices and techniques for high-risk human papillomavirus (HR-HPV) detection. The analytical performance to detect HR-HPV infections of this novel strategy was assessed by comparing it with the Hybrid Capture 2 system (HC2), which is used as gold standard. Methods Paired-cervical samples were collected from 541 women assisting to gynecological services in an outpatient clinic. One sample was transported in the Hybrid Capture Standard Transport Medium for HR-HPV detection by the HC2. The second sample was transported on glass slide for detection by PCR-based techniques (GP-EIA, BSGP-EIA and pU 1 M-L/2R). Results The level of agreement between the PCR-based techniques and HC2 system was determined with the Cohen’s kappa value. The kappa values between HC2 and GP-EIA, BSGP-EIA and pU 1 M-L/2R were 0.71 (CI 95% 0.63–0.78), 0.78 (CI 95% 0.71–0.84) and 0.63 (CI 95% 0.55–0.72), respectively. However, when the results from both BSGP-EIA and pU 1 M-L/2R were combined, the level of agreement with HC2 was increased to 0.82 (CI 95% 0.76–0.88), reflecting a very good agreement between the two HR-HPV detection strategies. Furthermore, the sensitivity of both techniques combined was also increased compared to the BSGP-EIA (88.7% vs 77.4%) and the pU (88.7 vs 60.9%) without penalizing the specificity obtained with the BSGP-EIA (95.1% vs 96.9%) and the pU (95.1% vs 96.5%). Conclusions This novel strategy, combining two PCR-based techniques for HR-HPV detection, could be useful for cervical cancer screening in self-collected samples in low-income countries.

show abstract

Human papillomavirus detection using PCR and ATR-FTIR for cervical cancer screening

Cited by 17 publications

References 42 publications

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

Biochemical detection of fatal hypothermia and hyperthermia in affected rat hypothalamus tissues by Fourier transform infrared spectroscopy

PCR-RFLP and FTIR-based detection of high-risk human papilloma virus for cervical cancer screening and prevention

Assessment of a new low-cost, PCR-based strategy for high-risk human papillomavirus DNA detection for cervical cancer prevention

Contact Info

Product

Resources

About