Human papillomavirus associated with bladder carcinoma? Analysis by polymerase chain reaction

Tekin, Mehmet İlteriş; Tunçer, Serdar; Aki, Fazıl Tuncay; Bilen, Cenk Yücel; Aygün, Cem; Özen, Haluk

doi:10.1046/j.1442-2042.1999.06435.x

Cited by 23 publications

(27 citation statements)

References 8 publications

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“…53 Bladder squamous cell carcinoma very rarely shows basaloid/warty features, which is consistent with the infrequent finding of HPV in bladder squamous cell carcinoma. In fact, it was these morphological features that lead us to perform HPV studies initially.…”

Section: Discussionsupporting

confidence: 83%

“…48,49 Other studies using the same methods yielded much lower rates of detection. 10,12,[50][51][52][53] Unfortunately, it is difficult to interpret the results because not all studies have analyzed non-lesional bladder tissue as control. For example, Anwar et al 9 detected the virus in 33% of healthy bladders, in contrast to Tekin, 53 who did not detect it in any of the 10 control cases.…”

Section: Discussionmentioning

confidence: 99%

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Urothelial carcinoma with prominent squamous differentiation in the setting of neurogenic bladder: role of human papillomavirus infection

et al. 2012

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Squamous cell carcinomas of the urinary bladder are rare in the Western world; the majority of cases are reported in countries endemic to Schistosoma parasitic infections. Unlike squamous tumors of the uterine cervix or oropharynx, the human papillomavirus (HPV) is not commonly associated with bladder squamous cell carcinomas. We report on two cases of HPV-positive urothelial carcinomas of the urinary bladder with extensive squamous differentiation showing the typical basaloid, poorly differentiated morphology of HPV-associated tumors. These occurred in patients with neurogenic bladders who had long-standing histories of selfcatheterization with tumors that tested positive for HPV by in situ hybridization. A retrospective review of our institutional database revealed four additional patients with bladder tumors showing squamous differentiation arising in the setting of neurogenic bladder. Review of these cases showed the more common welldifferentiated keratinizing appearance of squamous cell carcinomas of the bladder. These tumors showed only patchy positivity for p16 immunohistochemical stain (not the diffuse strong staining seen in HPV-positive tumors), and the one tested case was negative for HPV by in situ hybridization. HPV infection and neurogenic bladder have been independently associated with increased risk of developing carcinoma in the urinary bladder; however, this is the first report of squamous tumors arising in the setting of concurrent neurogenic bladder and HPV infection. The morphology of these tumors is similar to that of other high-risk HPV-associated squamous carcinomas with a basaloid, poorly differentiated appearance and little to no keratin formation. Modern Pathology (2012) 25, 1534-1542; doi:10.1038/modpathol.2012.112; published online 6 July 2012 Keywords: HPV; neurogenic bladder; squamous cell carcinoma; urothelial cancerThe human papillomavirus (HPV) has an important role as a human carcinogen, with an estimated 10% of cancers worldwide attributed to it. 1 HPV is a small circular double-stranded DNA virus with more than 100 different types described to date. 2 The potential of the virus to cause malignant or benign lesions dictates its classification into low-risk (6,11,40,42, and so on) or high-risk (16,18,31,33,45,51, and so on) types. The prototypical model for carcinogenesis is squamous cell carcinoma of the uterine cervix, in which nearly all such carcinomas are associated with HPV infection. 3,4 The oncogenic role of HPV is also well established for a subset of squamous cell carcinomas in the vulva, 5 penis, 6 anus, 7 and oropharynx. 8 The possible role of HPV infection in bladder tumors has not been completely elucidated as HPV detection rates vary widely from 0 to 100%, depending on the study. 9,10 An intriguing issue has been the possible association of the virus with bladder squamous cell carcinoma. The few published studies 11-14 on this relationship have shown a low frequency of HPV detection, implying that HPV is not likely to have a major role in the development of bladd...

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Urothelial carcinoma with prominent squamous differentiation in the setting of neurogenic bladder: role of human papillomavirus infection

et al. 2012

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“…Tekin et al, recently, stated that HPV did not seem to be related to the aetiology of bladder tumour due to its low prevalence reported in several previous studies (14), highlighting the possibility, as observed in cervical cancer studies by PCR, that positive results may be caused by superficial contamination of the tissue with HPV (15). Furthermore, Tekin et al suggested that in order to eliminate the possibility of contamination, future studies must be designed by using in situ PCR techniques, including samples taken from tumour and normal bladder mucosa in the same patient (14).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Tekin et al suggested that in order to eliminate the possibility of contamination, future studies must be designed by using in situ PCR techniques, including samples taken from tumour and normal bladder mucosa in the same patient (14). In the present study, in line with these suggestions, we analyzed samples taken both from tumour and urine, in order to eliminate the possibility of contamination and to demonstrate the feasibility and usefulness of HR-HPV DNA detected in the urine samples.…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus and non-muscle invasive urothelial bladder cancer: Potential relationship from a pilot study

Dan

2011

Oncol Rep

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Abstract.The relationship between urothelial bladder cancer and high-risk human papillomaviruses (HR-HPV) is still a poorly understood entity, even if some studies have supposed a probably correlation. The aim of the present study was to assess the potential relationship between the presence of HR-HPV and non-muscle invasive urothelial bladder cancers (NMIBC). One hundred and thirty-seven subjects (78 patients affected by NMIBC and 59 controls) were recruited in this prospective study. HR-HPV DNA was evaluated both in urine and tumour tissues. Data from patients were compared with data from controls. The relationship between patients and controls, in terms of HR-HPV presence was performed. The relationship between all pathological data and HR-HPV presence in patient group was carried out. HR-HPV DNA in tissue was found in 27 of 78 (34.6%) tumour samples and in 6 of 59 (10.1%) specimens from TUR-P, with a statistically significant difference (p=0.0009; dF=1; 2 =10.98). HR-HPV DNA in urine was found in 36 of 78 (46.1%) samples obtained from patients, whereas in only 8 of 59 (13.5%) samples from controls (p<0.0001: dF=1; 2 =16.37). A statistical significant difference in terms of HR-HPV frequency between high-grade and low-grade urothelial bladder cancer, was found (p=0.032; RR=0.52 -95% CI 0.27-0.93; OR=0.34 -95% CI 0.13-0.90). In conclusion, this study highlights the correlation between urothelial bladder cancer and high-risk type HPV infection, suggesting the potential etiopathogenetic role of HR-HPV in urothelial bladder cancer development. IntroductionApproximately 67,160 new bladder cancer cases are expected in the United States in 2007 with 13,750 estimated deaths (1).Moreover, the high recurrence rate and the percentage of adjuvant intravesical therapy failure contribute to bladder carcinoma becoming a social problem (2). In addition, following aspects of the urothelial bladder carcinoma natural history are partially unknown: i) whether a genetic factor exists which determines its predisposition (although there are several oncogenes involved), ii) which are the major risk factors involved in its genesis preventing us from putting into practice an effective prevention campaign. Among all etiopathogenetic factors and co-factors that contribute to urothelial bladder cancer development, we focused our attention on the role of human papillomavirus (HPV), which has been considered as a possible aetiologic agent (3). HPV is one of the most common sexually transmitted infections worldwide and it is associated with lesions ranging from benign cutaneous warts to malignancies, like cervical, penile or anal cancers (4). About 13 different HPV genotypes are associated with malignancy and are referred to as high-risk HPV (HR-HPV) (5,6). The oncogenic potential of HR-HPV types lies in the oncoproteins E6 and E7, which bind to and modulate a number of different gene products, in particular, the tumour suppressors p53 and pRb. These interactions lead to a disturbance of cell cycle control and a deficiency in DNA repair, res...

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“…6 Only a few studies have reported koilocytosis in tumors of urinary bladder; the changes have been described more frequently in low grade tumors. 4,5 The aim of this study was to correlate koilocytosis with HrHPV DNA in urinary bladder carcinoma and to compare detection of koilocytosis on tissue sections and urine cytology.…”

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confidence: 98%

Koilocytosis: Correlations with high‐risk HPV and its comparison on tissue sections and cytology, urothelial carcinoma

Aggarwal

Arora

Gupta

et al. 2009

Diagnostic Cytopathology

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The aim of this study were (1) To correlate koilocytosis with high risk HPV(HrHPV) DNA in urinary bladder carcinoma and (2) To compare detection of koilocytosis on tissue sections and urine cytology. Biopsy and cytologic specimens from 33 patients of urinary bladder carcinoma were analyzed. HPV DNA was detected by PCR on biopsy specimens using consensus primers MY09 and MY11. Koilocytosis was assessed both on tissue sections and urine cytology. HrHPV DNA was found in 14 of 33 bladder carcinoma. Koilocytosis was seen in tissue sections from 13 patients. Eleven of these were HrHPV DNA positive (positive predictive value 84.6%). Koilocytosis was seen in urine cytology in three patients. All three were positive for HrHPV DNA. To conclude koilocytosis is a good morphological marker for HrHPV DNA in the urothelium. Tissue sections are better than cytologic smears for detection of koilocytes.

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Human papillomavirus associated with bladder carcinoma? Analysis by polymerase chain reaction

Cited by 23 publications

References 8 publications

Urothelial carcinoma with prominent squamous differentiation in the setting of neurogenic bladder: role of human papillomavirus infection

Urothelial carcinoma with prominent squamous differentiation in the setting of neurogenic bladder: role of human papillomavirus infection

Human papillomavirus and non-muscle invasive urothelial bladder cancer: Potential relationship from a pilot study

Koilocytosis: Correlations with high‐risk HPV and its comparison on tissue sections and cytology, urothelial carcinoma

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