Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons

Abstract

Lactobacilli are thought to be beneficial for human health, with lactobacilli-associated infections being confined to immune-compromised individuals. However, Lactobacillus fermentum AGR1487 negatively affects barrier integrity in vitro so we hypothesized that it caused a pro-inflammatory response in the host. We compared germ-free rats inoculated with AGR1487 to those inoculated with another L. fermentum strain, AGR1485, which does not affect in vitro barrier integrity. We showed that rats inoculated with AGR1487 had more inflammatory cells in their colon, higher levels of inflammatory biomarkers, and increased colonic gene expression of pro-inflammatory pathways. In addition, our in vitro studies showed that AGR1487 had a greater capacity to activate TLR signaling and induce pro-inflammatory cytokines in immune cells. This study indicates the potential of strains of the same species to differentially elicit inflammatory responses in the host and highlights the importance of strain characterization in probiotic approaches to treat inflammatory disorders.

Sialoglycoprotein isolated from the eggs of Carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats

“…26 Lactobacillus strains have been widely recognized as probiotics, and a recent paper indicated that Lactobacillus fermentum AGR1487 induced inflammatory responses in the colon. 27 In conclusion, whether the alteration of the above gut bacteria in OVX model rats induced gut inflammation and the subsequent bone inflammation need to be further studied.…”

Sialoglycoprotein isolated from the eggs of Carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats

“…26 Lactobacillus strains have been widely recognized as probiotics, and a recent paper indicated that Lactobacillus fermentum AGR1487 induced inflammatory responses in the colon. 27 In conclusion, whether the alteration of the above gut bacteria in OVX model rats induced gut inflammation and the subsequent bone inflammation need to be further studied.…”

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

“…Numerous studies have reported that probiotics exert beneficial effects mainly through the recognition of the probiotics by TLRs, which activates corresponding signaling pathways and triggers appropriate responses [ 54 , 55 ]. The upregulation of the Nrf2 pathway, which protects against oxidative stress and injury, has been reported [ 56 ].…”

Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs

“…However, other studies have also reported that probiotics themselves can stimulate the immune system of the intestinal mucosa [70,71]. For example, Anderson et al (2016) showed that Lactobacillus fermentum AGR1487 can upregulate the expression of proin ammatory factors by activating the TLR signaling pathway and induce the in ammatory response in sterile rats. The different effects of microorganisms on the intestinal mucosal immune system may be due to the differences between test conditions and strains [72].…”

Sialoglycoprotein isolated from the eggs of Carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats

“…26 Lactobacillus strains have been widely recognized as probiotics, and a recent paper indicated that Lactobacillus fermentum AGR1487 induced inflammatory responses in the colon. 27 In conclusion, whether the alteration of the above gut bacteria in OVX model rats induced gut inflammation and the subsequent bone inflammation need to be further studied.…”

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

“…Numerous studies have reported that probiotics exert beneficial effects mainly through the recognition of the probiotics by TLRs, which activates corresponding signaling pathways and triggers appropriate responses [ 54 , 55 ]. The upregulation of the Nrf2 pathway, which protects against oxidative stress and injury, has been reported [ 56 ].…”

Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs

“…However, other studies have also reported that probiotics themselves can stimulate the immune system of the intestinal mucosa [70,71]. For example, Anderson et al (2016) showed that Lactobacillus fermentum AGR1487 can upregulate the expression of proin ammatory factors by activating the TLR signaling pathway and induce the in ammatory response in sterile rats. The different effects of microorganisms on the intestinal mucosal immune system may be due to the differences between test conditions and strains [72].…”

Sialoglycoprotein isolated from the eggs of Carassius auratus prevents bone loss: an effect associated with the regulation of gut microbiota in ovariectomized rats

“…26 Lactobacillus strains have been widely recognized as probiotics, and a recent paper indicated that Lactobacillus fermentum AGR1487 induced inflammatory responses in the colon. 27 In conclusion, whether the alteration of the above gut bacteria in OVX model rats induced gut inflammation and the subsequent bone inflammation need to be further studied.…”

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

“…Numerous studies have reported that probiotics exert beneficial effects mainly through the recognition of the probiotics by TLRs, which activates corresponding signaling pathways and triggers appropriate responses [ 54 , 55 ]. The upregulation of the Nrf2 pathway, which protects against oxidative stress and injury, has been reported [ 56 ].…”

Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs

“…However, other studies have also reported that probiotics themselves can stimulate the immune system of the intestinal mucosa [70,71]. For example, Anderson et al (2016) showed that Lactobacillus fermentum AGR1487 can upregulate the expression of proin ammatory factors by activating the TLR signaling pathway and induce the in ammatory response in sterile rats. The different effects of microorganisms on the intestinal mucosal immune system may be due to the differences between test conditions and strains [72].…”