Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons

Anderson, Rachel C.; Ulluwishewa, Dulantha; Young, Wayne; Ryan, Leigh; Henderson, Gemma; Meijerink, Marjolein; Maier, Eva; Wells, Jerry M.; Roy, Nicole C.

doi:10.1038/srep20318

Cited by 17 publications

(10 citation statements)

References 39 publications

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“…Numerous studies have reported that probiotics exert beneficial effects mainly through the recognition of the probiotics by TLRs, which activates corresponding signaling pathways and triggers appropriate responses [ 54 , 55 ]. The upregulation of the Nrf2 pathway, which protects against oxidative stress and injury, has been reported [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Chen

et al. 2021

Antioxidants

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Oxidative stress is increasingly being recognized as a player in the pathogenesis of intestinal pathologies, and probiotics are becoming an attractive means of addressing it. The present study investigated the effects of dietary supplementation with Lactobacillus delbrueckii (LAB) on intestinal integrity and oxidative damage in lipopolysaccharide (LPS)-challenged piglets. A total of 36 crossbred weaned piglets (Duroc × Landrace × Large Yorkshire) were randomly divided into three groups: (1) non-challenged controls (CON), (2) LPS-challenged controls (LPS), and (3) 0.2% LAB (2.01 × 1010 CFU/g) + LPS treatment (LAB + LPS). On the 29th day of the experiment, the LPS and CON groups were injected intraperitoneally with LPS and saline at 100 ug/kg body weight, respectively. The results show that the LPS-induced elevation of the serum diamine oxidase (DAO) level and small intestinal crypt depth (CD) were reversed by the dietary addition of LAB, which also markedly increased the ileal expression of tight junction proteins (occludin, ZO-1, and claudin-1) in the LPS-challenged piglets. Furthermore, LAB supplementation normalized other LPS-induced changes, such as by decreasing malondialdehyde (MDA) in both the serum and intestinal mucosa and 8-hydroxy-2-deoxyguanosine (8-OHdG) in the jejunal mucosa, increasing glutathione reductase (GR) and glutathione peroxidase (GSH-Px) in both the serum and intestinal mucosa, and increasing glutathione (GSH) and superoxide dismutase (SOD) in the jejunal mucosa. LAB also activated Toll-like receptor (TLR)–Bruton’s tyrosine kinase (Btk)–nuclear factor erythroid 2-related factor 2(Nrf2) signaling pathways in the intestine, suggesting that it plays a vital role in the ameliorative antioxidant capacity of weaned piglets. In summary, LAB increased intestinal integrity by improving the intestinal structure and tight junctions while enhancing antioxidant functions via the activation of the TLR–Btk–Nrf2 signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Chen

et al. 2021

Antioxidants

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“…However, other studies have also reported that probiotics themselves can stimulate the immune system of the intestinal mucosa [70,71]. For example, Anderson et al (2016) showed that Lactobacillus fermentum AGR1487 can upregulate the expression of proin ammatory factors by activating the TLR signaling pathway and induce the in ammatory response in sterile rats. The different effects of microorganisms on the intestinal mucosal immune system may be due to the differences between test conditions and strains [72].…”

Section: Discussionmentioning

confidence: 99%

Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs

Cao¹,

Tang²,

Zeng³

et al. 2020

Preprint

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Background: The previous study in our team found that supplementation of probiotic Bacillus amyloliquefaciens SC06 (BaSC06) as an alternative to antibiotics (Kitasamycin) can effectively reduce the diarrhea rate and promote the growth performance of weaned piglets. We speculate that BaSC06 may become a better substitute for antibiotics for fattening pigs. Hence, the objective of the present study is to evaluate the effects of BaSC06 substitute antibiotics on growth performance, digestive functions, antioxidant capacity, macrophage polarization and intestinal microbiota of fattening pigs. Methods: A total of 117 fattening pigs with similar weight and genetic basis were selected and divided into three groups: Anti group, fed with basal diet supplemented with 40 g/t Kitasamycin; Anti + Ba group, fed with 1 × 108 CFU/kg BaSC06 and 20 g/t Kitasamycin; Ba group, fed with 1 × 108 CFU/kg BaSC06 replaced Kitasamycin. Each treatment had three replications of 13 pigs per pen. Results: It indicated that BaSC06 replacement significantly improved ADFI and ADG in fattening pigs. It also improved intestinal digestion and absorption function with the increased activities of intestinal digestive enzymes and higher expression of glucose transporters SGLT1 and small peptide transporters PEPT1, maintained intestinal integrity significantly. Besides, supplementation with BaSC06 enhanced intestinal and body antioxidant capacity by activating Nrf2/ Keap1 antioxidant signaling pathway. In addition, BaSC06 as a substitute could alleviate intestinal inflammation by inhibiting the production of pro-inflammatory cytokines and chemokine and simultaneously increase the expression of M1 and M2 macrophage marker proteins in the intestinal mucosa. The transcription factor STAT3 regulated M2 polarization, which maintained immune homeostasis and reduced inflammation. What’s more, BaSC06 could improve the composition of the microbiota as well by increasing the proportion of Firmicutes, and reducing the proportion of Bacteroidetes and Proteobacteria. Conclusions: Taken together, our findings indicated that diet supplementation with BaSC06 instead of antibiotics increased the growth performance of piglets by improving the digestion and absorption of nutrients, intestinal microbiota composition and mucosal structure, antioxidative capacity and immune functions (including increasing M1and M2 polarization of macrophage).

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“…Closely, Anderson et al. () reported a human oral strain of Lactobacillus fermentum (AGR 1487) induced a proinflammatory response by damaging mucosal barrier integrity. The association of common probiotic genera with inflammation highlights the necessity of deeper taxonomic resolution when considering host–microbial interactions.…”

Section: Discussionmentioning

confidence: 99%

Salivary microbiome of an urban Indian cohort and patterns linked to subclinical inflammation

et al. 2017

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Human oral isolate Lactobacillus fermentum AGR1487 induces a pro-inflammatory response in germ-free rat colons

Cited by 17 publications

References 39 publications

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Lactobacillus delbrueckii Protected Intestinal Integrity, Alleviated Intestinal Oxidative Damage, and Activated Toll-Like Receptor–Bruton’s Tyrosine Kinase–Nuclear Factor Erythroid 2-Related Factor 2 Pathway in Weaned Piglets Challenged with Lipopolysaccharide

Effects of probiotic BaSC06 on intestinal digestion and absorption function, antioxidant capacity and macrophage polarization and microbiota composition in fattening pigs

Salivary microbiome of an urban Indian cohort and patterns linked to subclinical inflammation

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