Human oocyte cryopreservation: comparison between slow and ultrarapid methods

Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing

“…Of note, vitrification of GV oocytes resulted in more normal spindle structures after IVM than slow-freezing. For M-II oocytes, vitrification appears superior to slow-freezing with respect to viability and developmental outcomes [23,26,27]. Therefore, it is interesting that vitrification also performs better for GV oocytes, at least in the present study employing a single vitrification protocol.…”

“…Past clinical studies with GV oocytes from a variety of sources as well as freezing protocols have documented wide ranges for survival, maturation, fertilization, and pre-implantation development (slow-freezing: [7][8][9][10][11][12]; vitrification: [13][14][15][16]). For mature oocytes, advancements in protocols have resulted in encouraging outcomes, including slow-freezing with choline-substitution [17][18][19][20] and vitrification with the Cryoleaf system [15,[21][22][23][24]. However, there are limited prior reports of either cholinebased slow-freezing [11,25] or vitrification with the Cryoleaf [15] in human GV oocytes, with no previous assessments of spindle integrity or chromosome alignment.…”

“…Based on an increasing number of reports documenting benefits of vitrification over slow-freezing for the preservation of mature oocytes [23,26,27], we hypothesized that vitrification of immature oocytes would result in superior maturation and cytoskeletal outcomes when compared to slow-freezing.…”

Clinical outcomes from mature oocytes derived from preovulatory and antral follicles: reflections on follicle physiology and oocyte competence

“…Ovarian stimulation was carried out with rFSH (Merck Sharp & Dohme, Italy or Merck Serono, Italy), tailoring doses and duration of stimulation Values are mean ± standard deviation according to the patient's characteristics and needs [16]. Final oocyte maturation was triggered with 10,000 IU human hCG (Merck Sharp & Dohme, Italy) when at least three follicles with diameter ≥18 mm were visible at ultrasound scan monitoring.…”

Embryo transfer following in vitro maturation and cryopreservation of oocytes recovered from antral follicles during conservative surgery for ovarian cancer

“…Vitrification and successive warming was carried out as described elsewhere [8] using ethylene glycol, PROH and sucrose as cryoprotectants and cryoleaf as a carrier (Origio, Måløv, Denmark).…”