Human oligodendrocytes derived from embryonic stem cells: Effect of noggin on phenotypic differentiation in vitro and on myelination in vivo

Izrael, Michal; Zhang, Peilin; Kaufman, Rosalie; Shinder, Vera; Ella, Raya; Amit, Michal; Itskovitz‐Eldor, Joseph; Chebath, Judith; Revel, Michel

doi:10.1016/j.mcn.2006.11.008

Cited by 162 publications

(173 citation statements)

References 63 publications

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“…SB431542 inhibits the TGF␤/activin/ Lefty pathway by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors (47). Moreover, we have shown here that the addition of the bone morphogenetic protein antagonist Noggin, at a specific step in the neural induction of rabbit pluripotent stem cells, allows oligodendrocyte precursors to progress toward mature oligodendrocytes, as demonstrated previously in mouse and human ES cells (48). Thus, the effects of RA, SB431542, and Noggin on the neural induction of rabbit pluripotent stem cells have been confirmed, and we have emulated their effects on the pluripotent stem cells in mouse and human.…”

Section: Discussionsupporting

confidence: 82%

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Honda

Hatori

Hirose

et al. 2013

Journal of Biological Chemistry

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Background: The quality of induced pluripotent stem (iPS) cells might be inherently worse than embryonic stem cells. Results: Although the differentiation capacity of iPS cells is limited, it can be enhanced. Conclusion: Improving their level of pluripotency alleviated the limited capacity of iPS cells. Significance: This report offers an effective strategy for the development of iPS cell-based research.

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Section: Discussionsupporting

confidence: 82%

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Honda

Hatori

Hirose

et al. 2013

Journal of Biological Chemistry

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“…Human ES cells [199,200] can be directed into neural fate [201,202]. Recent studies have discovered the means to mimic the tightly controlled human embryonic developmental program to produce highly enriched populations of specific neurons [203][204][205][206] and oligodendrocytes [207,208]. Human embryonic stem cell-derived oligodendrocytes appear functional and capable of myelination [207,209].…”

Section: Embryonic Stem and Induced Pluripotential Cellsmentioning

confidence: 99%

Cell Therapy for Multiple Sclerosis

Ben‐Hur

2011

Neurotherapeutics

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The spontaneous recovery observed in the early stages of multiple sclerosis (MS) is substituted with a later progressive course and failure of endogenous processes of repair and remyelination. Although this is the basic rationale for cell therapy, it is not clear yet to what degree the MS brain is amenable for repair and whether cell therapy has an advantage in comparison to other strategies to enhance endogenous remyelination. Central to the promise of stem cell therapy is the therapeutic plasticity, by which neural precursors can replace damaged oligodendrocytes and myelin, and also effectively attenuate the autoimmune process in a local, nonsystemic manner to protect brain cells from further injury, as well as facilitate the intrinsic capacity of the brain for recovery. These fundamental immunomodulatory and neurotrophic properties are shared by stem cells of different sources. By using different routes of delivery, cells may target both affected white matter tracts and the perivascular niche where the trafficking of immune cells occur. It is unclear yet whether the therapeutic properties of transplanted cells are maintained with the duration of time. The application of neural stem cell therapy (derived from fetal brain or from human embryonic stem cells) will be realized once their purification, mass generation, and safety are guaranteed. However, previous clinical experience with bone marrow stromal (mesenchymal) stem cells and the relative easy expansion of autologous cells have opened the way to their experimental application in MS. An initial clinical trial has established the probable safety of their intravenous and intrathecal delivery. Short-term follow-up observed immunomodulatory effects and clinical benefit justifying further clinical trials.

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“…In these studies, the rate of donor-derived myelination was dependent on both developmental stage and purity of the cell population. However, as human OPCs cannot be readily expanded following isolation, various approaches have been used to specify OPC fate from embryonic stem cells (7,8). Although these procedures are able to generate enriched cultures of antigenically defined OPCs, specification of platelet-derived growth factor α receptor (PDGFαR)-expressing OPCs from OLIG2 + neural stem/progenitor cells (NPCs) requires more than 8 wk in culture, and the resulting cells initiate myelination at a significantly slower rate than native PDGFαR/CD140a-defined OPCs directly isolated from fetal human brain (5,9).…”

mentioning

confidence: 99%

Transcription factor induction of human oligodendrocyte progenitor fate and differentiation

Wang

Pol

Haberman

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Human oligodendrocyte progenitor cell (OPC) specification and differentiation occurs slowly and limits the potential for cell-based treatment of demyelinating disease. In this study, using FACS-based isolation and microarray analysis, we identified a set of transcription factors expressed by human primary CD140a + O4 + OPCs relative to CD133 + CD140a − neural stem/progenitor cells (NPCs). Among these, lentiviral overexpression of transcription factors ASCL1, SOX10, and NKX2.2 in NPCs was sufficient to induce Sox10 enhancer activity, OPC mRNA, and protein expression consistent with OPC fate; however, unlike ASCL1 and NKX2.2, only the transcriptome of SOX10-infected NPCs was induced to a human OPC gene expression signature. Furthermore, only SOX10 promoted oligodendrocyte commitment, and did so at quantitatively equivalent levels to native OPCs. In xenografts of shiverer/rag2 animals, SOX10 increased the rate of mature oligodendrocyte differentiation and axon ensheathment. Thus, SOX10 appears to be the principle and rate-limiting regulator of myelinogenic fate from human NPCs.neural precursor cell | transplantation | reprogramming

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Human oligodendrocytes derived from embryonic stem cells: Effect of noggin on phenotypic differentiation in vitro and on myelination in vivo

Cited by 162 publications

References 63 publications

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Naive-like Conversion Overcomes the Limited Differentiation Capacity of Induced Pluripotent Stem Cells

Cell Therapy for Multiple Sclerosis

Transcription factor induction of human oligodendrocyte progenitor fate and differentiation

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