Human neural stem cells: electrophysiological properties of voltage-gated ion channels

Down-regulation of MHC class I expression in human neuronal stem cells using viral stealth mechanism

“…This study carried out phenotypical and immunological characterization of a human neural stem cell line, named HB1.F3, which was previously reported to have stem cell properties [5,6,[27][28][29], and tested the feasibility of modifying the cells to have reduced levels of MHC class I antigens by the transfection of the hCMV genes, US2, 3, 6, and 11, into the NSCs in order to overcome the immune rejection in a future study of stem cell transplantation.…”

Noninvasive method of immortalized neural stem-like cell transplantation in an experimental model of Huntington's disease

“…Cytogenetic analyses performed on the established HB1 cell lines at various passages showed a normal human cell karyotype of 46, XX, without any chromosomal abnormality, and immunoreactivity for pan-myc oncogene protein, indicating that the HB1.F3 cells were uniquely of human origin and that they contained a copy or copies of v-myc-encoding retrovirus. Further electrophysiological characterization confirmed that HB1.F3 cells have the ability to differentiate to neurons (Cho et al, 2002).…”

“…These cells are tripolar or multipolar in morphology and 8-10 m in size. HB1.F3 cells express nestin and vimentin, both cell type specific markers for neural stem cells (Cho et al, 2002). Cytogenetic analyses performed on the established HB1 cell lines at various passages showed a normal human cell karyotype of 46, XX, without any chromosomal abnormality, and immunoreactivity for pan-myc oncogene protein, indicating that the HB1.F3 cells were uniquely of human origin and that they contained a copy or copies of v-myc-encoding retrovirus.…”

Functional properties of the human ventral mesencephalic neural stem cell line hVM1

“…Several studies have described amelioration of behavioral deficits by grafting such immortalized human stem cells in rodent models of neurological disease (Lee et al, 2007), and have characterized their immunocytochemical and biochemical features. However, few studies have addressed the functional properties of human neural stem cell lines (Jung et al, 1998;Cho et al, 2002;Lotharius et al, 2002;De Filippis et al, 2007;Donato et al, 2007), most of them failing to demonstrate * Corresponding author.…”

“…1 JT and EGS contributed equally to this study. maturation of these cells into neurons that can generate full-blown action potentials (Jung et al, 1998;Cho et al, 2002;De Filippis et al, 2007;Donato etal., 2007).…”