Human neural progenitor cells decrease photoreceptor degeneration, normalize opsin distribution and support synapse structure in cultured porcine retina

Mollick, Tanzina; Mohlin, Camilla; Johansson, Kjell

doi:10.1016/j.brainres.2016.06.039

Cited by 23 publications

(24 citation statements)

References 239 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, co-culturing the adult retina with ARPE cells decreased the amount of cell death, indicating that ARPE cells are beneficial for culture. These findings are well-known features that are seen when retinas are co-cultured with stem cells and progenitor cells ( 69 , 79 ). However, the degenerative processes are more predominant than the beneficial ones in cultures more than 3 days old.…”

Section: Discussionsupporting

confidence: 57%

“…In our experiments the adult porcine retinal photoreceptors were allowed to interact with the apical part of old ARPE cells, mimicking retinal degenerative diseases. Hence, culturing the retinal tissue over time in vitro led to a neurodegenerative process equivalent to a retinal degenerative process in vivo ( 69 , 79 , 80 ). In addition, co-culturing the adult retina with ARPE cells decreased the amount of cell death, indicating that ARPE cells are beneficial for culture.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

A model to study complement involvement in experimental retinal degeneration

Mohlin

Sandholm

Kvanta

et al. 2018

Upsala Journal of Medical Sciences

Self Cite

View full text Add to dashboard Cite

BackgroundThe complement system (CS) plays a role in the pathogenesis of a number of ocular diseases, including diabetic retinopathy (DR), glaucoma, uveitis, and age-related macular degeneration (AMD). Given that many of the complex eye-related degenerative diseases have limited treatment opportunities, we aimed to mimic the in vivo retinal degenerative process by developing a relevant co-culture system.Method and materialsThe adult porcine retina was co-cultured with the spontaneously arising human retinal pigment epithelial cells-19 (ARPE-19).ResultsInflammatory activity was found after culture and included migrating microglial cells, gliosis, cell death, and CS activation (demonstrated by a minor increase in the secreted anaphylotoxin C3a in co-culture). CS components, including C1q, C3, C4, soluble C5b-9, and the C5a receptor, were expressed in the retina and/or ARPE cells after culture. C1q, C3, and CS regulators such as C4 binding protein (C4BP), factor H (CFH), and factor I (CFI) were secreted after culture.DiscussionThus, our research indicates that this co-culturing system may be useful for investigations of the CS and its involvement in experimental neurodegenerative diseases.

show abstract

Section: Discussionsupporting

confidence: 57%

Section: Discussionmentioning

confidence: 99%

A model to study complement involvement in experimental retinal degeneration

Mohlin

Sandholm

Kvanta

et al. 2018

Upsala Journal of Medical Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…[ [187][188][189][190][191][192][193][194][195][196] FATTY ACIDS METABOLISM AND CIRCADIAN RHYTHMS NF-YA over-expression could alter the circadian rhythms regulation, as the daily cycle of Bmal1 expression and FASN signaling pathway. In order to link these results to RP pathogenesis, the altered FASN expression induced by oxidative stress may interfere with the normal RPE morphogenesis, leading to pathological phenotype.…”

Section: Retinoic Acid Cycle (Rdh5 Mkv)mentioning

confidence: 99%

RETRACTED ARTICLE: Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis

et al. 2018

View full text Add to dashboard Cite

Retinitis pigmentosa (RP) is a very heterogeneous inherited ocular disorder group characterized by progressive retinal disruption. Retinal pigment epithelium (RPE) degeneration, due to oxidative stress which arrests the metabolic support to photoreceptors, represents one of the principal causes of RP. Here, the role of oxidative stress in RP onset and progression was analyzed by a comparative whole transcriptome analysis of human RPE cells, treated with 100 µg/ml of oxLDL and untreated, at different time points. Experiment was thrice repeated and performed on Ion Proton TM sequencing system. Data analysis, including low quality reads trimming and gene expression quantification, was realized by CLC Genomics Workbench software. The whole analysis highlighted 14 clustered "macro-pathways" and many sub-pathways, classified by selection of 5271 genes showing the highest alteration of expression. Among them, 23 genes were already known to be RP causative ones (15 over-expressed and 8 down-expressed), and their enrichment and intersection analyses highlighted new 77 candidate related genes (49 over-expressed and 28 down-expressed). A final filtering analysis then highlighted 29 proposed candidate genes. This data suggests that many new genes, not yet associated with RP, could influence its etiopathogenesis.

show abstract

“…Supplementation of media with serum or neurotrophic factors, has promoted survival of retinal explants, suggesting that exogenous components may alter the natural progression of development and cell death. Co‐culture studies of wild‐type retinal explant tissue and hNPCs have shown better retinal structure and reduced microglial activation . Artifacts of wild‐type retinal degeneration in vitro may not represent disease modeling, and the use of inherent degenerative animal models could provide a more accurate picture of the progression of human retinal degeneration.…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Proteomic Comparison of Human Neural Progenitor Cell‐Induced Photoreceptor Survival

et al. 2019

View full text Add to dashboard Cite

Retinal degenerative diseases lead to blindness with few treatments. Various cell‐based therapies are aimed to slow the progression of vision loss by preserving light‐sensing photoreceptor cells. A subretinal injection of human neural progenitor cells (hNPCs) into the Royal College of Surgeons (RCS) rat model of retinal degeneration has aided in photoreceptor survival, though the mechanisms are mainly unknown. Identifying the retinal proteomic changes that occur following hNPC treatment leads to better understanding of neuroprotection. To mimic the retinal environment following hNPC injection, a co‐culture system of retinas and hNPCs is developed. Less cell death occurs in RCS retinal tissue co‐cultured with hNPCs than in retinas cultured alone, suggesting that hNPCs provide retinal protection in vitro. Comparison of ex vivo and in vivo retinas identifies nuclear factor (erythroid‐derived 2)‐like 2 (NRF2) mediated oxidative response signaling as an hNPC‐induced pathway. This is the first study to compare proteomic changes following treatment with hNPCs in both an ex vivo and in vivo environment, further allowing the use of ex vivo modeling for mechanisms of retinal preservation. Elucidation of the protein changes in the retina following hNPC treatment may lead to the discovery of mechanisms of photoreceptor survival and its therapeutic for clinical applications.

show abstract

Human neural progenitor cells decrease photoreceptor degeneration, normalize opsin distribution and support synapse structure in cultured porcine retina

Cited by 23 publications

References 239 publications

A model to study complement involvement in experimental retinal degeneration

A model to study complement involvement in experimental retinal degeneration

RETRACTED ARTICLE: Role of oxidative stress in Retinitis pigmentosa: new involved pathways by an RNA-Seq analysis

In Vitro and In Vivo Proteomic Comparison of Human Neural Progenitor Cell‐Induced Photoreceptor Survival

Contact Info

Product

Resources

About