Human muscle precursor cells give rise to functional satellite cells in vivo

Ehrhardt, Janine; Brimah, Karima; Adkin, C.; Partridge, Terence A.; Morgan, Jennifer E.

doi:10.1016/j.nmd.2007.04.009

Cited by 50 publications

(38 citation statements)

References 45 publications

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“…These human-derived satellite cells explain the presence of donorderived muscle-precursor cells evidenced in experiments of culture and retransplantation in immunodeficient mice [35] and confirm preliminary observations suggesting that some human nuclei in isolated human/mouse hybrid myofibers were satellite cells [36]. Human fetal myoblasts also produced mononucleated muscle-precursor cells following transplantation in immunodeficient mice [37].…”

Section: Generation Of Donor-derived Satellite Cellssupporting

confidence: 84%

Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

Skuk

2013

ISRN Transplantation

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Myopathies produce deficits in skeletal muscle function and, in some cases, progressive and irreversible loss of skeletal muscles. The transplantation of myogenic cells, that is, cells able to differentiate into myofibers, is an experimental strategy for the potential treatment of some of these diseases. The objectives pursued by the transplantation of these cells are essentially three: (a) the fusion with the patient's myofibers to obtain the expression of therapeutic proteins into them, (b) the neoformation of new functional myofibers in skeletal muscles that were too degenerated by the progressive degeneration, and (c) the formation of a new pool of healthy donor-derived satellite cells. Although the repertoire of myogenic cells appears to have expanded in recent years, myoblasts are the only cells that have been demonstrated to engraft in humans. The present work aims to make a comprehensive review of the subject, from its beginnings to recent advances, including the preclinical experience in different animal models and recent clinical findings.

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Section: Generation Of Donor-derived Satellite Cellssupporting

confidence: 84%

Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

Skuk

2013

ISRN Transplantation

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“…Within irradiated (but not in non-irradiated) dystrophic mdx hosts, the surviving donor cells proliferated, but nevertheless their contribution to regenerated muscle fibers was inefficient (Beauchamp et al 1999) compared with freshly isolated satellite cells . For technical reasons, it has not been possible to follow the kinetics of human mpc grafted into mouse muscles, but similar to mouse mpc (Morgan et al 1989(Morgan et al ,1990(Morgan et al ,1993Watt et al 1991;Gross and Morgan 1999), human mpc contribute to regenerated muscle fibers in immunodeficient mouse hosts (Huard et al 1994;Brimah et al 2004;SilvaBarbosa et al 2005;Ehrhardt et al 2007). However, human mpc repopulate host mouse muscle even less effectively than mouse mpc; fewer fibers of donor origin are found when the same number of human ) and mouse mpc (Morgan et al 2002;Cousins et al 2004) are grafted.…”

Section: Satellite Cell Contribution To Skeletal Muscle Regenerationmentioning

confidence: 99%

“…Mouse mpc contribute to significantly more muscle fibers of donor origin in mdx nu/nu than in C5-/Rag2-/ g chain-host mice (Morgan et al 2002). This implies that mouse and human mpc respond differently to an in vivo environment; human mpc may not undergo an expansion phase within the host mouse muscle and therefore neither regenerate skeletal muscle nor reconstitute the satellite cell niche efficiently (Ehrhardt et al 2007). …”

Section: Satellite Cell Contribution To Skeletal Muscle Regenerationmentioning

confidence: 99%

Are Human and Mouse Satellite Cells Really the Same?

Boldrin¹,

Muntoni²,

Morgan³

2010

J Histochem Cytochem.

117

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S U M M A R Y Satellite cells are quiescent cells located under the basal lamina of skeletal muscle fibers that contribute to muscle growth, maintenance, repair, and regeneration. Mouse satellite cells have been shown to be muscle stem cells that are able to regenerate muscle fibers and self-renew. As human skeletal muscle is also able to regenerate following injury, we assume that the human satellite cell is, like its murine equivalent, a muscle stem cell. In this review, we compare human and mouse satellite cells and highlight their similarities and differences. We discuss gaps in our knowledge of human satellite cells, compared with that of mouse satellite cells, and suggest ways in which we may advance studies on human satellite cells, particularly by finding new markers and attempting to re-create the human satellite cell niche in vitro. (J Histochem Cytochem 58:941-955, 2010)

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“…Human cells are routinely injected into immune-deficient mouse models of muscular dystrophy, such as the mdx4 cv and mdx5 cv models bred into the NSG or NOD/Rag1 null background (Darabi et al, 2012;Goudenege et al, 2012;; or into immunedeficient mice in which the recipient muscle has been damaged with cardiotoxin or by cryoinjury before injection (Cooper et al, 2001(Cooper et al, , 2003Brimah et al, 2004;Ehrhardt et al, 2007;Crisan et al, 2008;Negroni et al, 2009;Meng et al, 2010Meng et al, , 2011. After transplantation, the presence of engrafted human cells is detected via immunofluorescence staining using antibodies that recognize human, but not mouse, proteins.…”

mentioning

confidence: 99%

Mouse Regenerating Myofibers Detected as False-Positive Donor Myofibers with Anti-Human Spectrin

Rozkalne

Adkin²,

Meng³

et al. 2014

Human Gene Therapy

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Stem cell transplantation is being tested as a potential therapy for a number of diseases. Stem cells isolated directly from tissue specimens or generated via reprogramming of differentiated cells require rigorous testing for both safety and efficacy in preclinical models. The availability of mice with immune-deficient background that carry additional mutations in specific genes facilitates testing the efficacy of cell transplantation in disease models. The muscular dystrophies are a heterogeneous group of disorders, of which Duchenne muscular dystrophy is the most severe and common type. Cell-based therapy for muscular dystrophy has been under investigation for several decades, with a wide selection of cell types being studied, including tissue-specific stem cells and reprogrammed stem cells. Several immune-deficient mouse models of muscular dystrophy have been generated, in which human cells obtained from various sources are injected to assess their preclinical potential. After transplantation, the presence of engrafted human cells is detected via immunofluorescence staining, using antibodies that recognize human, but not mouse, proteins. Here we show that one antibody specific to human spectrin, which is commonly used to evaluate the efficacy of transplanted human cells in mouse muscle, detects myofibers in muscles of NOD/Rag1 null mdx 5cv, NOD/LtSz-scid IL2Rc null mice, or mdx nude mice, irrespective of whether they were injected with human cells. These ''reactive'' clusters are regenerating myofibers, which are normally present in dystrophic tissue and the spectrin antibody is likely recognizing utrophin, which contains spectrin-like repeats. Therefore, caution should be used in interpreting data based on detection of single human-specific proteins, and evaluation of human stem cell engraftment should be performed using multiple human-specific labeling strategies.

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Human muscle precursor cells give rise to functional satellite cells in vivo

Cited by 50 publications

References 45 publications

Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

Cell Transplantation and “Stem Cell Therapy” in the Treatment of Myopathies: Many Promises in Mice, Few Realities in Humans

Are Human and Mouse Satellite Cells Really the Same?

Mouse Regenerating Myofibers Detected as False-Positive Donor Myofibers with Anti-Human Spectrin

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