Human Mesenchymal Stem Cell Combined with a New Strontium-Enriched Bioactive Glass: An ex-vivo Model for Bone Regeneration

Bellucci, Devis; Veronesi, Elena; Strusi, Valentina; Petrachi, Tiziana; Murgia, Alba; Mastrolia, Ilenia; Dominici, Massimo; Cannillo, Valeria

doi:10.3390/ma12213633

Cited by 27 publications

(34 citation statements)

References 38 publications

(62 reference statements)

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“…Bioactive glasses were produced by a classical melt-quenching method, as previously described [8,9]. Briefly, commercial raw powders were melted at 1450 • C for 1 h in platinum crucibles (thermal cycle used: from room temperature to 1100 • C at 10 • C/min; an isothermal step for 1 h at 1100 • C; from 1100 • C to 1450 • C at 10 • C/min).…”

Section: Preparation Of Bioactive Glassesmentioning

confidence: 99%

“…The 3D cellular model, used to mimic the potential clinical application of a given biomaterial, was fully described in previous works [8,9] and is here only briefly summarized. Such model used human bone marrow mesenchymal stem cells, which were isolated from bone marrow of 3 healthy donors and then expanded ex vivo.…”

Section: Biologic Characterizationmentioning

confidence: 99%

“…The performance of bioactive glasses in such 3D cellular models was evaluated by using human bone marrow mesenchymal stem cells (BM-MSCs). The results pointed out that the two bioactive glasses supported BM-MSCs adhesion, proliferation and bone differentiation [8,9]. In particular, the model showed that granules of bioactive glass Bio_MS were able to stimulate the osteogenic differentiation of BM-MSCs, even without proper growth factors, thus revealing the osteoinductivity and the regenerative potential of the material [9].…”

Section: Introductionmentioning

confidence: 96%

“…Recently, an innovative 3D cellular model, able to reproduce the potential clinical application of a given material, was used for the first time to investigate novel bioactive glasses, enriched with strontium and magnesium, namely BGMS10 [8] and Bio_MS [9]. The compositions of such glasses in mol% were respectively: 2.…”

Section: Introductionmentioning

confidence: 99%

“…The main goal of this paper is to test, for the first time, the "gold standard" 45S5 by means of the innovative 3D cellular model, with the double aim of: (i) validating such model with reference to the well-known 45S5, for which a lot of results are available in the literature, also in terms of in vivo experiments; (ii) comparing the performance of the two novel bioactive glasses [8,9] with the standard 45S5. In fact, since for the 45S5 in vivo experiments are available, the comparison of the output of the present model with literature data could be useful to assess the viability of such methodology and its reliability.…”

Section: Introductionmentioning

confidence: 99%

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On the in Vitro Biocompatibility Testing of Bioactive Glasses

et al. 2020

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In this work, a new 3D cellular model—based on human bone marrow mesenchymal stem cells (BM–MSCs)—was used for the first time to test the 45S5 Bioglass® (45S5). Such a model, previously used to evaluate the biologic performance of two novel bioactive glasses, suggested out the regenerative potential of such materials. In fact, BM–MSCs were able both to adhere and colonize the biomaterials, as well as differentiate toward osteoblasts—even in absence of specific growth factors. Surprisingly, BM–MSCs were not able to colonize 45S5 granules (almost no adhesion and/or colonization was observed), and thus, were not capable of providing any osteogenic differentiation. Therefore, the model seems to suggest that the two novel bioactive glasses have a better biologic performance than 45S5. If this hypothesis is confirmed also by in vivo tests, the 3D model may become a predictive tool for discriminating between different potential bioactive materials by comparatively evaluating them, and preliminarily selecting the best ones in relation to their biocompatibility potential—before proceeding with further experiments in vivo. This approach could favor the reduction of costs and time of pre-clinical and clinical trials.

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Section: Preparation Of Bioactive Glassesmentioning

confidence: 99%

Section: Biologic Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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On the in Vitro Biocompatibility Testing of Bioactive Glasses

et al. 2020

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In‐vivo evaluations of bone regenerative potential of two novel bioactive glasses

Anesi

Ferretti

Salvatori

et al. 2023

J Biomedical Materials Res

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Due to the aging of population, materials able to repair damaged tissues are needed. Among others, bioactive glasses (BGs) have attracted a lot of interest due to their outstanding properties both for hard and soft tissues. Here, for the first time, two new BGs, which gave very promising results in preliminary in vitro‐tests, were implanted in animals in order to evaluate their regenerative potential. The new BGs, named BGMS10 and Bio_MS and containing specific therapeutic ions, were produced in granules and implanted in rabbits' femurs for up to 60 days, to test their biocompatibility and osteoconduction. Additionally, granules of 45S5 Bioglass® were employed and used as a standard reference for comparison. The results showed that, after 30 days, the two novel BGs and 45S5 displayed a similar behavior, in terms of bone amount, thickness of new bone trabeculae and affinity index. On the contrary, after 60 days, 45S5 granules were mainly surrounded by wide and scattered bone trabeculae, separated by large amounts of soft tissue, while in BGMS10 and Bio_MS the trabeculae were thin and uniformly distributed around the BG granules. This latter scenario could be considered as more advantageous, since the features of the two novel BG granules allowed for the neo‐formation of a uniformly distributed bony trabeculae, predictive of more favorable mechanical behavior, compared to the less uniform coarse trabeculae, separated by large areas of soft tissue in 45S5 granules. Thus, BGMS10 and Bio_MS could be considered suitable products for tissue regeneration in the orthopedic and dental fields.

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