Human mast cells stimulate fibroblast proliferation, collagen synthesis and lattice contraction: a direct role for mast cells in skin fibrosis

Garbuzenko, Ekaterina; Nagler, Arnon; Pickholtz, Dalia; Gillery, Philippe; Reich, Reuven; Maquart, F X; Levi‐Schaffer, Francesca

doi:10.1046/j.1365-2222.2002.01293.x

Cited by 201 publications

(147 citation statements)

References 42 publications

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“…26 Mast cells clearly play a role in regulating extracellular matrix metabolism during injury repair. Mast cell extracts have previously been shown in vitro to stimulate the production of collagen by skin fibroblasts, 27 and they are also capable of activating MMP activity, for instance via tryptase-mediated cleavage of MMP3. 28 An altered balance of collagen synthesis and secretion in response to mast cell activity would clearly affect the quantity and organizational properties of repair tissue.…”

Section: Mast Cells In Patellar Tendon Injurymentioning

confidence: 99%

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Sharma

Abraham

Sampaio

et al. 2010

Journal Orthopaedic Research

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The purpose of this study was to determine whether administration of a mast cell inhibitor (sodium cromolyn, SC) would influence tendon repair and extracellular matrix gene expression following acute injury. CD1 mouse patellar tendons were unilaterally injured and mast cell prevalence was determined. The effect of SC injection on tendon hypercellularity, cross-sectional area, collagen organization, and expression of extracellular matrix-related genes was examined. Mast cell prevalence was markedly increased in injured patellar tendons ( p ¼ 0.009), especially at 8 weeks post-injury ( p ¼ 0.025). SC injection increased collagen organization compared to uninjected animals at 4 weeks and attenuated the development of tendon hypercellularity and tendon thickening post-injury. Expression of CTGF, ADAMTS1, and TIMP3 in injured tendon was reduced in the SC group. SC injections moderated the structural alterations of healing tendon in association with downregulation of several genes associated with tendon fibrosis. This work corroborates previous findings pointing to a role of mast cells in tendon repair. ß

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Section: Mast Cells In Patellar Tendon Injurymentioning

confidence: 99%

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Sharma

Abraham

Sampaio

et al. 2010

Journal Orthopaedic Research

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“…The potential of mast cells to play a role in chronic asthma and airway remodeling is suggested from studies demonstrating an important role for mast cells in tissue fibrosis, including pulmonary fibrosis (10,11). Several mast cell-derived mediators are candidates for playing a role in mediating airway remodeling based on their demonstrated ability in vitro to influence fibroblast proliferation or collagen synthesis (12)(13)(14). For example, both histamine and tryptase stimulate fibroblast proliferation as well as collagen synthesis (12)(13)(14).…”

mentioning

confidence: 99%

“…Several mast cell-derived mediators are candidates for playing a role in mediating airway remodeling based on their demonstrated ability in vitro to influence fibroblast proliferation or collagen synthesis (12)(13)(14). For example, both histamine and tryptase stimulate fibroblast proliferation as well as collagen synthesis (12)(13)(14). In addition TGF-␤, a key cytokine-regulating fibrosis, is also generated by mast cells (15).…”

mentioning

confidence: 99%

Accumulation of Peribronchial Mast Cells in a Mouse Model of Ovalbumin Allergen Induced Chronic Airway Inflammation: Modulation by Immunostimulatory DNA Sequences

Ikeda

Miller

Nayar

et al. 2003

The Journal of Immunology

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Few peribronchial mast cells are noted either in the lungs of naive mice or in the lungs of OVA-sensitized mice challenged acutely with OVA by inhalation. In this study, we demonstrate that OVA-sensitized mice exposed to repetitive OVA inhalation for 1–6 mo have a significant accumulation of peribronchial mast cells. This accumulation of peribronchial mast cells is associated with increased expression of the Th2 cell-derived mast cell growth factors, including IL-4 and IL-9, but not with the non-Th2 cell-derived mast cell growth factor, stem cell factor. Pretreating mice with immunostimulatory sequences (ISS) of DNA containing a CpG motif significantly inhibited the accumulation of peribronchial mast cells and the expression of IL-4 and IL-9. To determine whether mast cells express Toll-like receptor-9 (TLR-9; the receptor for ISS), TLR-9 expression by mouse bone marrow-derived mast cells (MBMMCs) was assessed by RT-PCR. MBMMCs strongly expressed TLR-9 and bound rhodamine-labeled ISS. However, incubation of MBMMCs with ISS in vitro neither inhibited MBMMC proliferation nor inhibited Ag/IgE-mediated MBMMC degranulation, but they did induce IL-6. Overall these studies demonstrate that mice exposed to repetitive OVA challenge, but not acute OVA challenge, have an accumulation of peribronchial mast cells and express increased levels of mast cell growth factors in the lung. Although mast cells express TLR-9, ISS does not directly inhibit mast cell proliferation in vitro, suggesting that ISS inhibits accumulation of peribronchial mast cells in vivo by indirect mechanism(s), which include inhibiting the lung expression of Th2 cell-derived mast cell growth factors.

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“…[29,30] Mast cells have been associated with secretion of a number of important mediators of fibrosis. [31,32] It was shown that mast cells were present in higher numbers in inflammatory fibrous hyperplasia, as they were responsible for the adaptive and pathological responses in areas on chronic inflammation. [33] This was attributed due to the presence of a number of chemical mediators including histamines, proteases, chymase and tryptase within the secretory granules of the mast cells.…”

Section: Hande X 100mentioning

confidence: 99%

Fibrous Epulis Misdiagnosed for Combined Periodontic-Endodontic Lesion

Khzam¹,

Mansouri²,

Poli³

et al. 2017

International Journal of Medical and Dental Sciences

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Human mast cells stimulate fibroblast proliferation, collagen synthesis and lattice contraction: a direct role for mast cells in skin fibrosis

Cited by 201 publications

References 42 publications

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Sodium cromolyn reduces expression of CTGF, ADAMTS1, and TIMP3 and modulates post‐injury patellar tendon morphology

Accumulation of Peribronchial Mast Cells in a Mouse Model of Ovalbumin Allergen Induced Chronic Airway Inflammation: Modulation by Immunostimulatory DNA Sequences

Fibrous Epulis Misdiagnosed for Combined Periodontic-Endodontic Lesion

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