Human mast cells arise from a common circulating progenitor

Lin− CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitors

“…There was also a tendency that the transcripts for the mast cell a-and b-tryptases (Tpsab1 and Tpsb2) and carboxypeptidase A (Cpa3) were all expressed to a higher degree in the mast cell progenitors than in the basophils, even though there were no significant differences in these transcripts ( Figure 4A). To provide further evidence for the commitment to the mast cell lineage, qRT-PCR analysis of proteases that are expressed in mast cells derived from human blood 20 and serglycin (Srgn), the core protein for heparin/ chondroitin sulfate E proteoglycans, was performed on the primary mast cell progenitors. Tryptase (Tpsab1/Tpsb2), carboxypeptidase (Cpa3), and serglycin (Srgn) were detected ( Table 2).…”

“…The primers for the proteases were previously published. 20 qRT-PCR was performed using SYBR GreenER SuperMix (ThermoFisher), 200 nM primers, following recommended PCR cycling conditions. Melting curve analysis was performed to ensure product uniformity.…”

“…119, 20 and CD34 1 CD117 121 progenitor cells in peripheral blood by in vitro culture. However, whether human mast cells originate from a distinct population of progenitors has not previously been determined.…”

Adipose triglyceride lipase regulates eicosanoid production in activated human mast cells

“…All experiments were carried out with mature MCs (i.e., with cells that had been allowed to differentiate for 6 to 8 weeks) ( 17 ).…”

Lipolysis meets inflammation: arachidonic acid mobilization from fat

“…However, more recent evidence suggests that MCs are also involved in infl ammatory reactions associated with obesity, atherosclerosis, autoimmune disorders, and cancer ( 1 ). MCs derive from hematopoietic stem cells, circulate and migrate as precursors, and terminally mature in their target tissues ( 2 ). There, MCs reside as quiescent cells until activated by antigen-presenting immunoglobulin E or other nonimmunological stimuli.…”