Human Mammospheres Secrete Hormone-Regulated Active Extracellular Vesicles

González, Esperanza; Piva, Marco; Rodríguez-Suárez, Eva; Gil, David; Royo, Félix; Elortza, Félix; Falcón‐Pérez, Juan Manuel; Vivanco, María dM

doi:10.1371/journal.pone.0083955

Cited by 15 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As mammary tissues are hormonal target organs, studies of EV in human mammary cells could provide useful insights into how steroid hormones regulate oEV protein content in human Fallopian tubes. In cultured human mammary epithelial cells, E2 treatment led to the secretion of smaller exosomes and increased protein expression levels of exosome markers such as CD63 and FLOT-1, CD133, ANXA2, catalase, and high molecular weighted ubiquitinated proteins [52]. This study indicates that E2 impacts EV secretion and protein content in human cells.…”

Section: Hormonal Regulation Of Oevsmentioning

confidence: 55%

Extracellular Vesicles and the Oviduct Function

Harris

Stephens

Winuthayanon

2020

Preprint

View full text Add to dashboard Cite

In mammals, the oviduct (or the Fallopian tube in humans) can be divided into the infundibulum (responsible for oocyte pick-up), ampulla (site of fertilization), isthmus (where preimplantation embryos develop), and uterotubal junction (where embryos transit to the uterus). The oviductal fluid, as well as extracellular vesicles produced from the oviductal epithelial cells, referred to as oEVs, have been shown to improve the fertilization process, prevent polyspermy, and aid in embryo development. oEVs contain molecular cargos (such as miRNAs, mRNAs, proteins, and lipids) that can be delivered and fuse to recipient cells. oEVs produced from the ampulla appear to be functionally distinct from those produced from the isthmus. In multiple species including mice, cats, dogs, pigs, and cows, oEVs can be incorporated into the oocytes, sperm, and embryos. In this review, we show the positive impact of oEVs on gamete function as well as blastocyst development and how they may improve embryo quality in in vitro conditions in an assisted reproductive technology setting for rodents, domestic animals, farm animals, and humans.

show abstract

Section: Hormonal Regulation Of Oevsmentioning

confidence: 55%

Extracellular Vesicles and the Oviduct Function

Harris

Stephens

Winuthayanon

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Although secretion of EVs by primary human MaSCs grown as mammospheres has been described previously, that study only focused on EVs smaller than 0.2 m (i.e. exosomes) (24). Here, we describe the secretion of MVs, larger vesicles of ϳ0.2-2 m in size, from both equine and canine MaSCs.…”

Section: Discussionmentioning

confidence: 90%

“…To the best of our knowledge, only one recent study has described the secretion of EVs by human MaSCs. In that instance cells were grown in short term mammosphere cultures, and only EVs smaller than 220 nm were analyzed (24). Because most MVs are larger than 220 nm, we first isolated and characterized the MVs secreted by eMaSCs and/or cMaSCs.…”

Section: Resultsmentioning

confidence: 99%

Microvesicle-mediated Wnt/β-Catenin Signaling Promotes Interspecies Mammary Stem/Progenitor Cell Growth

Bussche

Rauner

Antonyak

et al. 2016

Journal of Biological Chemistry

View full text Add to dashboard Cite

Signaling mechanisms that regulate mammary stem/progenitor cell (MaSC) self-renewal are essential for developmental changes that occur in the mammary gland during pregnancy, lactation, and involution. We observed that equine MaSCs (eMaSCs) maintain their growth potential in culture for an indefinite period, whereas canine MaSCs (cMaSCs) lose their growth potential in long term cultures. We then used this system to investigate the role of microvesicles (MVs) in promoting self-renewal properties. We found that Wnt3a and Wnt1 were expressed at higher levels in MVs isolated from eMaSCs compared with those from cMaSCs. Furthermore, eMaSC-MVs were able to induce Wnt/β-catenin signaling in different target cells, including cMaSCs. Interestingly, the induction of Wnt/β-catenin signaling in cMaSCs was prolonged when using eMaSC-MVs compared with recombinant Wnt proteins, indicating that MVs are not only important for transport of Wnt proteins, but they also enhance their signaling activity. Finally, we demonstrate that the eMaSC-MVs-mediated activation of the Wnt/β-catenin signaling pathway in cMaSCs significantly improves the ability of cMaSCs to grow as mammospheres and, importantly, that this effect is abolished when eMaSC-MVs are treated with Wnt ligand inhibitors. This suggests that this novel form of intercellular communication plays an important role in self-renewal.

show abstract

“…Sample analysis was carried out with a JEM 2200 F (JEOL) transmission cryo-electron microscope, using an acceleration voltage of 200 KV and defocus ranging from 21.2 to 22.5 mm, determined accurately by using enhanced power spectra. Images were taken with 2k62k TM Ultrascan 1000 CCD camera (Gatan) 76 .…”

Section: Methodsmentioning

confidence: 99%

Generation of a new infectious recombinant prion: a model to understand Gerstmann–Sträussler–Scheinker syndrome

Elezgarai

Fernández‐Borges

Eraña

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Human transmissible spongiform encephalopathies (TSEs) or prion diseases are a group of fatal neurodegenerative disorders that include Kuru, Creutzfeldt-Jakob disease, Gerstmann-Sträussler-Scheinker syndrome (GSS), and fatal familial insomnia. GSS is a genetically determined TSE caused by a range of mutations within the prion protein (PrP) gene. Several animal models, based on the expression of PrPs carrying mutations analogous to human heritable prion diseases, support that mutations might predispose PrP to spontaneously misfold. An adapted Protein Misfolding Cyclic Amplification methodology based on the use of human recombinant PrP (recPMCA) generated different self-propagating misfolded proteins spontaneously. These were characterized biochemically and structurally, and the one partially sharing some of the GSS PrPSc molecular features was inoculated into different animal models showing high infectivity. This constitutes an infectious recombinant prion which could be an invaluable model for understanding GSS. Moreover, this study proves the possibility to generate recombinant versions of other human prion diseases that could provide a further understanding on the molecular features of these devastating disorders.

show abstract

Human Mammospheres Secrete Hormone-Regulated Active Extracellular Vesicles

Cited by 15 publications

References 42 publications

Extracellular Vesicles and the Oviduct Function

Extracellular Vesicles and the Oviduct Function

Microvesicle-mediated Wnt/β-Catenin Signaling Promotes Interspecies Mammary Stem/Progenitor Cell Growth

Generation of a new infectious recombinant prion: a model to understand Gerstmann–Sträussler–Scheinker syndrome

Contact Info

Product

Resources

About