Extracellular Vesicles and the Oviduct Function

Harris, Emily A.; Stephens, Kalli K.; Winuthayanon, Wipawee

doi:10.20944/preprints202010.0638.v1

Cited by 17 publications

(6 citation statements)

References 68 publications

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“…In the uterus, stromal‐derived IGF1 stimulates epithelial cell proliferation through a paracrine mechanism 11 . It was previously shown that secreted factors present in the extracellular vesicles can diffuse through the zona pellucida and bind to the plasma membrane of bovine preimplantation embryos 73 . Therefore, it is possible that in the mouse oviduct, secreted IGF1 can be diffused in a similar fashion as in the uterus through epithelial cells and promote embryo development in the oviductal lumen.…”

Section: Discussionmentioning

confidence: 99%

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

et al. 2021

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One of the endogenous estrogens, 17β-estradiol (E 2 ) is a female steroid hormone secreted from the ovary. It is well established that E 2 causes biochemical and histological changes in the uterus. However, it is not completely understood how E 2 regulates the oviductal environment in vivo. In this study, we assessed the effect of E 2 on each oviductal cell type, using an ovariectomized-hormone-replacement mouse model, single-cell RNA-sequencing (scRNA-seq), in situ hybridization, and celltype-specific deletion in mice. We found that each cell type in the oviduct responded to E 2 distinctively, especially ciliated and secretory epithelial cells. The treatment of exogenous E 2 did not drastically alter the transcriptomic profile from that of endogenous E 2 produced during estrus. Moreover, we have identified and validated genes of interest in our datasets that may be used as cell-and region-specific markers in the oviduct. Insulin-like growth factor 1 (Igf1) was characterized as an E 2 -target gene in the mouse oviduct and was also expressed in human fallopian tubes. Deletion of Igf1 in progesterone receptor (Pgr)-expressing cells resulted in female subfertility, partially due to an embryo developmental defect and embryo retention within the oviduct. In summary, we have shown that oviductal cell types, including epithelial, stromal, and muscle cells, are differentially regulated by E 2 and support gene expression changes, such as growth factors that are required for normal embryo development and transport in mouse models. Furthermore, we have identified cell-specific and region-specific gene markers for targeted studies and functional analysis in vivo. K E Y W O R D Sembryo development, embryo transport, estrogen, insulin-like growth factor 1, oviduct, scRNA-seq 2 of 19 | MCGLADE Et AL.

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Section: Discussionmentioning

confidence: 99%

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

et al. 2021

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“…EVs, referred to as exosomes (30–150 nm) and microvesicles (>150–1000 nm). EVs are present in oviduct fluids and have emerged as a new method of cell‐to‐cell communication (Harris et al, 2020). Oviductal EVs have been identified as crucial components of the OF and potential mediators of embryonic‐maternal interactions (Almiñana & Bauersachs, 2019; Bridi et al, 2020; Lee et al, 2021; Saadeldin et al, 2021, 2014, 2015).…”

Section: Introductionmentioning

confidence: 99%

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Fang

Tanga

Bang

et al. 2021

Molecular Reproduction Devel

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Extracellular vesicles (EVs) carry bioactive cargoes involved in the early preimplantation development. This study investigated the effects of EVs obtained from an oviductal epithelial cell (OEC) conditioned medium on the developmental competence of in parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) porcine embryos. The OEC‐EV‐treated group showed significant increases in blastocyst formation and hatching rates compared to the control group (40.8% ± 2.2% and 20.1% ± 2.1% vs. 24.9% ± 2.0% and 5.3% ± 1.1%; p < 0.05), respectively. The 7 day OEC‐EVs treatment group significantly increased blastocyst formation rate than the 3 day and 0 day‐groups (45.0 ± 0.8 vs. 33.0 ± 0.7 and 26.7 ± 0.5; p < 0.05), respectively. SCNT revealed that the OEC‐EV increased blastocyst formation rate compared to that of oviductal fluid EVs (OF‐EVs) (35.4% ± 1.4% vs. 29.3% ± 1.3%; p < 0.05). Reactive oxygen species levels, apoptosis, and blastocyst lipid content were significantly decreased in the OEC‐EVs group compared with the control group. OEC‐EV group showed a significantly decreased BAX and increased BCL2, SOD1, POU5F1, SOX2, NANOG, GATA6, PNPLA2, LIPE, and MGLL gene expression than the control group (p < 0.05). In conclusion, OEC‐EVs supplementation in embryo culture media improved the quality of porcine embryos, potentially helping porcine‐cloned embryonic development possibly through transfer of messenger RNA and proteins to the early embryos.

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“…However, there are numerous hints that apocrine and paracrine signaling play a major role. In regard to apocrine signaling, it is widely accepted that the oviductal cells produce extracellular vesicles (EVs), which include exosomes and microvesicles (reviewed by Alminana and Bauersachs and Harris et al 48,49 …”

Section: The Sperm Reservoirmentioning

confidence: 99%

Sperm‐oviduct interactions: Key factors for sperm survival and maintenance of sperm fertilizing capacity

Kölle

2022

Andrology

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Background: Although millions or even billions of sperm are deposited in the female genital tract, only very few sperm reach the oocyte, and only one single spermatozoon will successfully fertilize. During the journey of the sperm within the female genital tract, the interactions between spermatozoa and fallopian tube are critical for sperm selection, sperm survival, and maintenance of sperm fertilizing capacity.Results: This review will provide a comprehensive overview of the latest findings regarding sperm transport and behavior of sperm within the oviduct, sperm selection in the oviduct, the formation of the sperm reservoir, and the release of sperm in the presence of the oocyte. It will primarily focus on recent novel insights on sperm-oviduct interactions, which have been obtained by cutting-edge technologies under in vivo or near in vivo conditions. Conclusions:The comprehensive analysis of the findings to date will elucidate the complex molecular changes in the tubal epithelium, which are induced by the presence of the sperm and will highlight how the epithelial cells of this organ affect transport, behavior, and function of sperm. This knowledge is essential for scientists and clinicians involved in assisted reproductive technologies.

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Extracellular Vesicles and the Oviduct Function

Cited by 17 publications

References 68 publications

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

Oviduct epithelial cells‐derived extracellular vesicles improve preimplantation developmental competence of in vitro produced porcine parthenogenetic and cloned embryos

Sperm‐oviduct interactions: Key factors for sperm survival and maintenance of sperm fertilizing capacity

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