Human macrophage-mediated biodegradation of polyurethanes: assessment of candidate enzyme activities

Labow, Rosalind S.; Meek, Erin; Matheson, Loren A.; Santerre, J. Paul

doi:10.1016/s0142-9612(02)00137-0

Cited by 88 publications

(93 citation statements)

References 20 publications

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“…[89] Studies of this phenomenon by Labow and Santerre have focused on the ability of macrophage-derived enzymes to facilitate degradation of polyurethanes. [90][91][92][93][94][95] These studies clearly identified the importance of the use of antioxidants in these polymers to inhibit the oxidation process that occurs with the foreign body reaction. The persistence of the foreign body reaction and the fact that it is present at the interface between the tissue and the device for the lifetime of the device, suggests that the oxidation process is continuous, albeit at low levels.…”

Section: Device Failurementioning

confidence: 92%

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Foreign body reaction to biomaterials

Anderson

Rodriguez

Chang

2008

Seminars in Immunology

4,029

3,868

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The foreign body reaction composed of macrophages and foreign body giant cells is the end-stage response of the inflammatory and wound healing responses following implantation of a medical device, prosthesis, or biomaterial. A brief, focused overview of events leading to the foreign body reaction is presented. The major focus of this review is on factors that modulate the interaction of macrophages and foreign body giant cells on synthetic surfaces where the chemical, physical, and morphological characteristics of the synthetic surface are considered to play a role in modulating cellular events. These events in the foreign body reaction include protein adsorption, monocyte/ macrophage adhesion, macrophage fusion to form foreign body giant cells, consequences of the foreign body response on biomaterials, and cross-talk between macrophages

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Section: Device Failurementioning

confidence: 92%

“…The soft segment of polyether polyurethanes is known to undergo degradation. [86][87][88][89][90][91][92][93][94][95][96][97][98] This phenomenon will be discussed in the next section.…”

Section: Mediators Of Degradation-mentioning

confidence: 97%

Foreign body reaction to biomaterials

Anderson

Rodriguez

Chang

2008

Seminars in Immunology

4,029

3,868

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“…It was previously reported that cholesterol esterase and carboxyl esterase activities increased during long term culture of macrophages with polycarbonate urethane [18,19]. As the structure of PLGA is also abundant in ester bonds, it could be postulated that macrophages are capable of mediating degradation of Dextran/PLGA scaffolds through a comparable mechanism.…”

Section: Assessment Of Enzyme Activitiesmentioning

confidence: 99%

“…On the contrary, since most synthetic polymers are not specific substrates for natural enzymes, the mechanisms of enzyme-mediated polymer degradation are likely to be complex. Limited attention has been devoted to discerning the activities of enzymes on polymer degradation [18,19] and many other related issues have yet to be explored. It was previously reported that the activities of various capsule-borne enzymes were elevated in a rat subdermal model two weeks after material implantation [20].…”

Section: Assessment Of Enzyme Activitiesmentioning

confidence: 99%

The biodegradability of electrospun Dextran/PLGA scaffold in a fibroblast/macrophage co-culture

2008

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Fibroblast and macrophage are two dominant cell types respond cooperatively to degrade implanted biomaterials. Using an electrospun Dextran/Poly-lactide-co-glycolide (PLGA) scaffold as a model, an in vitro fibroblast/macrophage co-culture system was developed to investigate the degradability of implantable biodegradable materials. SEM showed that both fibroblasts and macrophages were able to degrade the scaffold, separately or cooperatively. Under the synergistic coordination of macrophages and fibroblasts, scaffolds showed faster degradation rate than their counterparts incubated with a single type of cells as well as in PBS or cell culture medium. Lysozyme, non-specific esterase (NSE), gelatinase, hyaluronidase-1 and α-glucosidase were upregulated in the presence of the scaffold, suggesting their roles in the cell-mediated scaffold degradation. In addition, the expressions of cell surface receptors CD204 and Toll like receptor 4 (TLR4) were elevated one week after cell seeding, implying that these receptors might be involved in scaffold degradation. The results of in vivo subdermal implantation of the scaffold further confirmed the biodegradability of the Dextran/PLGA scaffold. The fibroblast/macrophage co-culture model adequately mimicked the in vivo environment and could be further developed into an in vitro tool for initial biomaterial evaluation.

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“…1 However, the applications of PU in certain implant devices and particularly long-term implants have been limited because of their relative instability in the bioenvironment. 2 When acute inflammatory response is induced by PU implant, monocyte-derived macrophages that release reactive oxygen species are activated. They are the most abundant cells at the surface of PU implanted devices.…”

Section: Introductionmentioning

confidence: 99%

Preparation of collagen–polyurethane composite film and its subcutaneous implantation in rats: The improvement of tissue compatibility

Li¹,

Huang²

2005

J of Applied Polymer Sci

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This paper reports a safe, easy, effective, and one-step process to introduce a collagen layer onto a polyester-urethane surface for improving its biocompatibility and reducing acute inflammatory reaction. Collagen gel (COL) was spread onto the plasma-treated polyurethanes (PU) film to make PU-COL composite film by lyophilization. In this process, collagen on the interface was covalently immobilized to PU surface. Density of immobilized collagen molecules was examined to find the optimal experiment condition. The surface properties of the immobilized film were characterized by attenuated total reflection infrared spectrum and X-ray photoelectron spectroscopy to determine the efficiency of collagen immobilization. The results indicated that collagen chains had been grafted on PU surface because of plasma activation. To see if collagen modification can deduce acute inflammatory reaction and improve tissue guide regeneration, PU and PU-COL composite film were implanted subdermally in rats to analyze the effect of collagen immobilization. The reaction interface of PU-COL composite film and rat's tissue was observed by transmission electron microscope to analyze biocompatibility of PU-COL; unmodified PU film was used as control. The result showed that acute inflammatory reaction induced by PU-COL composite film had vanished gradually after 7 days and the material was embedded by tissue, almost forming a capsule. The capsule's wall thinned out gradually in the following days. Although the control group's inflammatory reaction did not vanish in 1 month and PU film embed in rat's tissue incompletely, PU implant migrated easily from the implant site. As a result, PU-COL composite film had most advantage in tissue guide regeneration and compatibility.

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Human macrophage-mediated biodegradation of polyurethanes: assessment of candidate enzyme activities

Cited by 88 publications

References 20 publications

Foreign body reaction to biomaterials

Foreign body reaction to biomaterials

The biodegradability of electrospun Dextran/PLGA scaffold in a fibroblast/macrophage co-culture

Preparation of collagen–polyurethane composite film and its subcutaneous implantation in rats: The improvement of tissue compatibility

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