Human Leukocyte Antigens (HLA) Genes Association in Type 1 Diabetic Nephropathy

Abstract

Background: Diabetic nephropathy is a common worldwide multifactorial disease where involvement of genetic factors is well etablished. The aim of this study was to investigate the HLA genes implication in the development of type 1 diabetic nephropathy. Methods: We performed a case- control study where one hundred and fifty subjects were examined. Patients were divided in two groups; with and without type 1 diabetic nephropathy. HLA typing was performed using Polymerase Chain Reaction- Sequence Specific Oligonucleotide (PCR- SSO) method. HLA association to clinical phenotype and HLA haplotype analysis was also investigated. Results: HLA B*51 is increased in patients without type 1 diabetic nephropathy (7.14% vs. 0 %, P <0.05, OR= 0), however no other studied alleles seem to have any effect (all P>0.05). Haplotype analysis also does not reveal any significant association, however, A*02-B*18-DRB1*03-DQA1*05- DQB1*03 haplotype shows a tendency to be associated with the development of diabetic nephropathy (P = 0.05). Conclusion: These results suggest a protective effect of HLA B*51 allele from type 1 diabetic nephropathy. However, further studies are required in order to clarify its potential implication as a protective marker.

Identification of a novel immune landscape signature as effective diagnostic markers related to immune cell infiltration in diabetic nephropathy

“…It can be a logical explanation for our study that CD8+ T cells and MHC class I antigen were coincidentally elevated in the DN group. Moreover, dendritic cells, HLA, neutrophils, Th1 cells, Th2 cells, and so on, were demonstrated to play a crucial role in the development and process of DN (11,76,[81][82][83][84]. In view of this, our research is consistent with previous reports and highlights the importance of those immune-related cells and types in the pathogenesis of DN through bioinformatic analysis.…”