Human Islet Isolation in 104 Consecutive Cases

Py, Benhamou; Pc, Watt; Mullen, Yoko; Ingles, Sue A.; Watanabe, Yasumasa; Nomura, Yuji; Hober, C; Miyamoto, Masaaki; Kenmochi, Takashi; Ep, Passaro

doi:10.1097/00007890-199457120-00021

Cited by 124 publications

(38 citation statements)

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“…Few analyses of islet isolation have been reported [4][5][6][7][8]. Consistent with the multivariate analysis of our data, most of these reports also found that high numbers of islets were best obtained from donors with high BMI [4,5,7,8,17,18].…”

Section: Discussionsupporting

confidence: 90%

“…Consistent with the multivariate analysis of our data, most of these reports also found that high numbers of islets were best obtained from donors with high BMI [4,5,7,8,17,18]. This condition is often considered to be a marker of insulin resistance and of high metabolic demand, both of which represent a stimulus for islet neogenesis (beta cell adaptation) that increases the overall native beta cell mass [19].…”

Section: Discussionsupporting

confidence: 85%

“…Pancreas digestion, and in particular the choice of collagenase, are critical for isolation success [7,20]. The use of batches with high collagenase activity per total enzyme was an important determinant for successful isolation.…”

Section: Discussionmentioning

confidence: 99%

“…Although isolation has progressively improved, islet yield is highly variable. Poor donor selection, non-standardised procedures and the inconsistency of enzyme blends used for digestion are some of the parameters that contribute to this variability [4][5][6][7][8]. Analysis of enzyme blends and the islet isolation parameters that predict outcome have not been assessed.…”

Section: Introductionmentioning

confidence: 99%

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Islet isolation for allotransplantation: variables associated with successful islet yield and graft function

et al. 2005

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Aims/hypothesis: Efficient islet isolation is an important prerequisite for successful clinical islet transplantation. Although progressively improved, islet yield and quality are, however, unpredictable and variable and require standardisation. Methods: Since 1989 we have processed 437 pancreases using the automated method. The donor characteristics, pancreas procurement, and digestion and purification procedures including a wide enzyme characterisation of these pancreases were analysed and correlated with islet yield and transplant outcome. Results: By univariate analysis, islet yield was significantly associated with donor age (r=0.16; p=0.0009), BMI (r=0.19; p= 0.0004), good pancreas condition (p=0.0031) and weight (r=0.15; p=0.0056), total collagenase activity (r=0.22; p= 0.0001), adjusted collagenase activity/mg (r=0.18; p=0.0002), collagenase activity/solution volume (r=0.18; p=0.0002) and neutral protease activity/solution volume (r=0.14; p= 0.0029). A statistically significant contribution to the variability of islet yield in a multivariate analysis performed on donor variables was found for donor BMI (p=0.0008). In a multivariate analysis performed on pancreas variables a contribution was found for pancreas weight (p=0.0064), and for a multivariate analysis performed on digestion variables we found a contribution for digestion time (p= 0.0048) and total collagenase activity (p=0.0001). Twenty-four patients with type 1 diabetes received single islet preparations from single donors. In these patients, multivariate analyses showed that the reduction in insulin requirement was significantly associated with morphological aspects of islets (p= 0.0010) and that 1-month C-peptide values were associated with islet purity (p=0.0071). Conclusions/interpretation: These data provide baseline donor, digestion and purification selection criteria for islet isolation using the automated method and indicate that the morphological aspect may be a clinically relevant measure of islets on which the decision for transplant can be based.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Islet isolation for allotransplantation: variables associated with successful islet yield and graft function

et al. 2005

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“…Donor characteristics and pancreas procurement are the first steps. The quality of the donor pancreas depends largely on donor factors, such as age, body mass index, serum glucose levels, and hemodynamic stability [5] . In the procurement phase, improved surgical techniques such as in-vivo atraumatic dissection and in-situ separation have made sufficient advancements in minimizing warm ischemia before isolation.…”

Section: Great Efforts To Optimize Islet Transplantationmentioning

confidence: 99%

Islet transplantation and antioxidant management: A comprehensive review

Monfared¹,

Larijani

Abdollahi

2009

WJG

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Islet transplantation as a promising treatment for type 1 diabetes has received widespread attention. Oxidative stress plays an essential role in cell injury during islet isolation and transplantation procedures. Antioxidants have been used in various studies to improve islet transplantation procedures. The present study reviews the role of oxidative stress and the benefits of antioxidants in islet transplantation procedures. The bibliographical databases Pubmed and Scopus were searched up to November 2008.All relevant human and animal in-vivo and in-vitro studies, which investigated antioxidants on islets, were included. Almost all the tested antioxidants used in the in-vitro studies enhanced islet viability and insulin secretion. Better control of blood glucose after transplantation was the major outcome of antioxidant therapy in all in-vivo studies. The data also indicated that antioxidants improved islet transplantation procedures. Although there is still insufficient evidence to draw definitive conclusions about the efficacy of individual supplements, the benefits of antioxidants in islet isolation procedures cannot be ignored.

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