Islet Yield Remains a Problem in Islet Autotransplantation

Morrison, Charles; Wemyss‐Holden, Simon A.; Dennison, Ashley R.; Maddern, Guy J.

doi:10.1001/archsurg.137.1.80

Cited by 38 publications

(13 citation statements)

References 23 publications

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“…The somewhat higher number of isolated islets per rat strain in the present study (614 6 253 for AO and 303 6 46 for Wistar) compared to the retrospectively analysed studies can be attributed to the fact that more animals were isolated at the same time during the previous studies. This leads to longer procurement time of the organs, which is associated with lower islet yields (van der Burg et al 1994a, Morrison et al 2002. These data show that using AO instead of Lewis, Wistar or SD rats can halve the number of animals necessary for experimental studies.…”

Section: Discussionmentioning

confidence: 96%

Rat islet isolation yield and function are donor strain dependent

et al. 2004

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Islet transplantation has proven to be a potential cure for insulin-dependent diabetic patients (Shapiro et al. 2000). Islet isolation is crucial for successful islet transplantation. Islet mass, islet quality and islet purity are determined by islet isolation ef cacy, which depends on many factors, including organ procurement, collagenase digestion and puri cation method (Williams et al. 1995, Vos-Scheperkeuter et al. 1997, Morrison et al. 2002. Apart from differences in islet yield among species (van Suylichem et al. 1995, Wieczorek et al. 1998, variability in islet yield within one species is also found (van Deijnen et al. 1992). These variations can be attributed to organ quality (van der Burg et al. 1994b), batch-to-batch variation in collagenase preparation (Wolters et al. 1990(Wolters et al. , 1992, and donor age

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Section: Discussionmentioning

confidence: 96%

Rat islet isolation yield and function are donor strain dependent

et al. 2004

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“…Possible reasons for incomplete islet recovery include the destructive effects of enzymatic digestion, a central phase of the isolation process (9,10). During pancreatic digestion, islets are cleaved from their surrounding matrix and subjected to mechanical and chemical events that can cause cell loss (4,11).…”

Section: Introductionmentioning

confidence: 99%

Flexible Management of Enzymatic Digestion Improves Human Islet Isolation Outcome from Sub‐Optimal Donor Pancreata

Balamurugan

Chang

Fung

et al. 2003

American Journal of Transplantation

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Worldwide growing interest in reproducing the result of the Edmonton protocol in islet transplantation trials poses the problem of paucity of donors to supply sufficient amount of islets for clinical use. Improved outcomes include finding better ways to obtain higher yields from every donor organ processed and the possibility of extending islet isolation processing to glands of suboptimal quality. In order to optimize enzymatic digestion of marginal donor organs, we have modified the technique of tissue collection following enzymatic digestion of human pancreatic organs, allowing for reduced time of exposure of free islets to warm Liberase TM solution. Our results indicate that better controlled exposure to enzyme yields: (i) higher islet numbers; (ii) complete dissociation of all parts of pancreatic tissue; (iii) successful islet harvest from organs otherwise excluded. We also show that by limiting the exposure of free islets to enzyme solution, islet fragmentation and loss of insulin content are reduced. We further support evidence that enzymatic digestion may contribute to impairment of insulin secretory capacity of the islets in vitro during culture.

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“…Similarly, also celsior and HTK solution have proven valuable in this context [13]. However, despite recent modifications and improvements in islet isolation techniques, it is still often possible to isolate only 50-70% of the total number of islets from a human pancreas [14,15]. To what extent this is influenced by an inadequate preservation of islets is at present unknown and is the rationale for this study.…”

Section: Discussionmentioning

confidence: 99%

“…However, recently also other preservation solutions such as HTK solution [8] and colloid-free celsior solution have proven to be adequate for this purpose [13]. However, despite recent modifications and improvements in islet isolation techniques, it is still often possible to isolate only 50-70% of the total number of islets in a human pancreas [14,15]. To what extent this is influenced by an inadequate preservation of islets due to poor penetration of preservation media is at present unknown.…”

Section: Introductionmentioning

confidence: 99%