Human Islet Co-Cultured with Bone Marrow Mesenchymal Stem Cells in 3D Scaffolding May Augment Pancreatic Beta Cell Function

Kim, JosephW.; Vang, Souriya; Luo, JohnZ. Q.; Luo, Liqiang

doi:10.1166/jbt.2017.1557

Cited by 4 publications

(3 citation statements)

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“…Few studies have been conducted using agarose as a 3D scaffold. One group recently showed the effect of co-culturing bone marrow derived mesenchymal stem cells and human islets on agarose gel which proved application of agarose in pancreatic tissue engineering [42]. But as agarose gel does not support growth of cells, agarose alone might not fit the criteria.…”

Section: Discussionmentioning

confidence: 99%

Long Term Sustained Growth of Insulin Producing Cells Using Agarose-Chitosan Coated Silver Nanocomposites

Ganguly¹

2021

Series Endo Diab Met.

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Diabetes is a group of diseases characterized by high levels of blood sugar for an extended period. Despite newer and effective therapy, current treatment is riddled with fundamental challenges. To overcome the adverse effects of existing drugs, regenerative medicine has emerged as an essential treatment, for which tissue engineering may serve as a foundation for the repair of pancreatic cells secreting insulin. Different polymeric scaffolds have been explored for pancreatic tissue engineering. In the current study, a continuation of our preceding work we attempt to test the role of previously synthesized agarose-chitosan coated silver nanocomposite scaffold (AG-CHNp) for the long-term growth of pancreatic cells. Pancreatic cells were isolated from BALB/c mice and were characterized by dithizone (DTZ) staining, real time polymerase chain reaction (RT-PCR), western blotting, and flow cytometry for characteristic pancreatic markers. The isolated population of cells was grown on scaffolds and its effectiveness towards insulin secretion was studied. The isolated population was found to be positive for glucagon, PDX-1 and Pax-4, while a 200-fold change transcript level of insulin was observed. The cells upon seeding on the scaffolds exhibited sustained growth and insulin secretion as confirmed by western blotting. Overall, the study demonstrates the suitability and application of AG-CHNp for pancreatic tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Long Term Sustained Growth of Insulin Producing Cells Using Agarose-Chitosan Coated Silver Nanocomposites

Ganguly¹

2021

Series Endo Diab Met.

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“…Transplantation of β-cells alone has shown limited success. Coculture with other cell types such as fibroblasts or MSCs have shown improved viability, functionality, and insulin secretion [41,68,90]. However, the major problem associated with islet transplantation is still substantial cell loss post-isolation and again post-transplantation due to hypoxia-induced apoptosis, loss of suitable microenvironment, and immune response [142][143][144].…”

Section: Discussionmentioning

confidence: 99%

“…This study highlighted the role of BM-MSCs and agarose gel in improving the overall functionality of islets. It is suggested that BM-MSCs provide growth factors and paracrine signaling, and agarose gel allows cells to absorb nutrients in an unlimited manner which improves islet function [68].…”

Section: Agarosementioning

confidence: 99%

Polymeric Scaffolds for Pancreatic Tissue Engineering: A Review

2017

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In recent years, there has been an alarming increase in the incidence of diabetes, with one in every eleven individuals worldwide suffering from this debilitating disease. As the available treatment options fail to reduce disease progression, novel avenues such as the bioartificial pancreas are being given serious consideration. In the past decade, the research focus has shifted towards the field of tissue engineering, which helps to design biological substitutes for repair and replacement of non-functional or damaged organs. Scaffolds constitute an integral part of tissue engineering; they have been shown to mimic the native extracellular matrix, thereby supporting cell viability and proliferation. This review offers a novel compilation of the recent advances in polymeric scaffolds, which are used for pancreatic tissue engineering. Furthermore, in this article, the design strategies for bioartificial pancreatic constructs and their future applications in cell-based therapy are discussed.

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Bone Marrow Mesenchymal Stem Cells as a New Therapeutic Approach for Diabetes Mellitus

Kim

Luo²,

Luo

2019

A Roadmap to Non-Hematopoietic Stem Cell-Based Therapeutics

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Human Islet Co-Cultured with Bone Marrow Mesenchymal Stem Cells in 3D Scaffolding May Augment Pancreatic Beta Cell Function

Cited by 4 publications

References 0 publications

Long Term Sustained Growth of Insulin Producing Cells Using Agarose-Chitosan Coated Silver Nanocomposites

Long Term Sustained Growth of Insulin Producing Cells Using Agarose-Chitosan Coated Silver Nanocomposites

Polymeric Scaffolds for Pancreatic Tissue Engineering: A Review

Bone Marrow Mesenchymal Stem Cells as a New Therapeutic Approach for Diabetes Mellitus

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