Human iPS Cell-Derived Germ Cells: Current Status and Clinical Potential

Ishii, Tetsuya

doi:10.3390/jcm3041064

Cited by 23 publications

(17 citation statements)

References 84 publications

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“…The molecules that are involved in GC commitment, such as BMP4 and Wnt3, have been identified and PGCs are induced from pluripotent stem cells under the control of these molecules and other cell differentiation‐inducing factors . In humans, PGCs also are induced in similar culture conditions . The induced mouse PGCs can be maintained in a normal manner and differentiated into spermatozoa and oocytes with the ability to develop to term .…”

Section: Resultsmentioning

confidence: 99%

Recent advances of in vitro culture systems for spermatogonial stem cells in mammals

Sahare

Suyatno

Imai

2018

Reprod Medicine & Biology

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BackgroundSpermatogonial stem cells (SSCs) in the mammalian testis are unipotent stem cells for spermatozoa. They show unique cell characteristics as stem cells and germ cells after being isolated from the testis and cultured in vitro. This review introduces recent progress in the development of culture systems for the establishment of SSC lines in mammalian species, including humans.MethodsBased on the published reports, the isolation and purification of SSCs, identification and characteristics of SSCs, and culture system for mice, humans, and domestic animals have been summarized.ResultsIn mice, cell lines from SSCs are established and can be reprogrammed to show pluripotent stem cell potency that is similar to embryonic stem cells. However, it is difficult to establish cell lines for animals other than mice because of the dearth of understanding about species‐specific requirements for growth factors and mechanisms supporting the self‐renewal of cultured SSCs. Among the factors that are associated with the development of culture systems, the enrichment of SSCs that are isolated from the testis and the combination of growth factors are essential.ConclusionProviding an example of SSC culture in cattle, a rational consideration was made about how it can be possible to establish cell lines from neonatal and immature testes.

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Section: Resultsmentioning

confidence: 99%

Recent advances of in vitro culture systems for spermatogonial stem cells in mammals

Sahare

Suyatno

Imai

2018

Reprod Medicine & Biology

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“…This strategy finds its roots in the work of Takahashi and Yamanaka (2006), where adult mouse fibroblasts in culture could be reprogrammed into pluripotent cells (iPSC for induced pluripotent stem cells), the later being able to develop into the three embryonic germ layers. From then on, many groups tried to reprogram differentiated cells into other types of differentiated cells via iPSCs, including germ cells (Ishii, 2014). The most advanced works are found in mouse (Cai et al, 2013; Imamura et al, 2010) and human (Ishii, 2014).…”

Section: Somatic Cells: Strength and Pitfalls Of Preservation And mentioning

confidence: 99%

“…From then on, many groups tried to reprogram differentiated cells into other types of differentiated cells via iPSCs, including germ cells (Ishii, 2014). The most advanced works are found in mouse (Cai et al, 2013; Imamura et al, 2010) and human (Ishii, 2014). Although fully functional gametes could not be obtained yet, this strategy should not be forgotten among the panel of biotechnologies for fish regeneration from somatic cells.…”

Section: Somatic Cells: Strength and Pitfalls Of Preservation And mentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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“…Induction of germ cells from humaninduced pluripotent (iPS) cells is also an encouraging technique in this field. It has been reported that generation of haploid round spermatids from human iPS cells can be achieved in vitro [102]. Further progress will contribute to the development of novel therapeutic techniques for NOA patients in the future.…”

Section: Future Prospectsmentioning

confidence: 99%

Management of non‐obstructive azoospermia

Chiba

Enatsu

Fujisawa

2016

Reprod Medicine & Biology

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Non-obstructive azoospermia (NOA) is defined as no sperm in the ejaculate due to failure of spermatogenesis and is the most severe form of male infertility. The etiology of NOA is either intrinsic testicular impairment or inadequate gonadotropin production. Chromosomal or genetic abnormalities should be evaluated because there is a relatively high incidence compared with the normal population. Although rare, NOA due to inadequate gonadotropin production is a condition in which fertility can be improved by medical treatment. In contrast, there is no treatment that can restore spermatogenesis in the majority of NOA patients. Consequently, testicular extraction of sperm under an operating microscope (micro-TESE) has been the firstline treatment for these patients. Other treatment options include varicocelectomy for NOA patients with a palpable varicocele and orchidopexy if undescended testes are diagnosed after adulthood, although management of these patients remains controversial. Advances in retrieving spermatozoa more efficiently by micro-TESE have been made during the past decade. In addition, recent advances in biotechnology have raised the possibility of using germ cells produced from stem cells in the future. This review presents current knowledge about the etiology, diagnosis, and treatment of NOA.

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Human iPS Cell-Derived Germ Cells: Current Status and Clinical Potential

Cited by 23 publications

References 84 publications

Recent advances of in vitro culture systems for spermatogonial stem cells in mammals

Recent advances of in vitro culture systems for spermatogonial stem cells in mammals

Cryobanking of aquatic species

Management of non‐obstructive azoospermia

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