Human intervention study to investigate the intestinal accessibility and bioavailability of anthocyanins from bilberries

Mueller, Dolores; Jung, Kathrin; Winter, Manuel; Rogoll, Dorothee; Melcher, Ralph; Richling, Elke

doi:10.1016/j.foodchem.2017.03.130

Cited by 108 publications

(121 citation statements)

References 45 publications

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“…In these animal studies, the anti‐inflammatory effects were tentatively attributed to the presence of blueberry polyphenols such as anthocyanins and/or proanthocyanidins . However, recent human studies have consistently shown that blueberry polyphenols, including anthocyanins, flavan‐3‐ols, proanthocyanidins and flavonols, have limited bioavailability . In contrast, their metabolites, mainly the phenolic acids, can be detected in plasma at higher concentrations relative to the parent polyphenols.…”

Section: Introductionmentioning

confidence: 99%

Physiological Concentrations of Blueberry‐Derived Phenolic Acids Reduce Monocyte Adhesion to Human Endothelial Cells

Tang

Bozonet

McKenzie

et al. 2019

Molecular Nutrition Food Res

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Scope: Blueberry polyphenols are thought to confer cardiovascular health benefits, but have limited bioavailability. They undergo extensive metabolism and their phenolic acid metabolites are likely to be the mediators of bioactivity. The effect of blueberry-derived phenolic acids on one aspect of inflammation, monocyte adhesion to vascular endothelial cells, is investigated. Methods and results: The major blueberry-derived phenolic acids in human plasma are identified and quantified. Three test mixtures representing compounds present at 0-4 h (Early), 4-24 h (Late), or 0-24 h (Whole) are used to investigate the effect on adhesion of monocytes to tumor necrosis factor alpha (TNFα)-activated endothelial cells. The Late mixture reduces monocyte adhesion, but there is no effect of the Early or Whole mixtures. Exclusion of syringic acid from each mixture results in inhibition of monocyte adhesion. Exposure to the phenolic acid mixtures has no effect on the endothelial surface expression of adhesion molecules intercellular cell adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), or E-selectin, suggesting that other molecular mechanisms are responsible for the observed effect. Conclusion: This study shows that physiological concentrations of blueberry polyphenol metabolites can help maintain cardiovascular health by regulating monocyte adhesion to the vascular endothelium.

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Section: Introductionmentioning

confidence: 99%

Physiological Concentrations of Blueberry‐Derived Phenolic Acids Reduce Monocyte Adhesion to Human Endothelial Cells

Tang

Bozonet

McKenzie

et al. 2019

Molecular Nutrition Food Res

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“…Therefore, anthocyanins have a great potential in various fields, such as the pharmaceutical and food industries. However, the relatively low stability and bioavailability of anthocyanins are the primary barrier to limit the wide and effective applications [9][10][11], raising up the importance of reducing the degradation of anthocyanins and controlling release. So far, encapsulation [12][13][14][15], composite [16][17][18][19] and group substitution [20][21][22][23][24] are the three main means used for anthocyanin stabilization.…”

Section: Introductionmentioning

confidence: 99%

Preparation and pH Controlled Release of Fe3O4/Anthocyanin Magnetic Biocomposites

et al. 2019

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Anthocyanins are a class of antioxidants extracted from plants, with a variety of biochemical and pharmacological properties. However, the wide and effective applications of anthocyanins have been limited by their relatively low stability and bioavailability. In order to expand the application of anthocyanins, Fe 3 O 4 /anthocyanin magnetic biocomposite was fabricated for the storage and release of anthocyanin in this work. The magnetic biocomposite of Fe 3 O 4 magnetic nanoparticle-loaded anthocyanin was prepared through physical intermolecular adsorption or covalent cross-linking. Scanning electron microscopy (SEM), Dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and thermal analysis were used to characterize the biocomposite. In addition, the anthocyanin releasing experiments were performed. The optimized condition for the Fe 3 O 4 /anthocyanin magnetic biocomposite preparation was determined to be at 60 • C for 20 h in weak alkaline solution. The smooth surface of biocomposite from SEM suggested that anthocyanin was coated on the surface of the Fe 3 O 4 particles successfully. The average size of the Fe 3 O 4 /anthocyanin magnetic biocomposite was about 222 nm. Under acidic conditions, the magnetic biocomposite solids could be repeatable released anthocyanin, with the same chemical structure as the anthocyanin before compounding. Therefore, anthocyanin can be effectively adsorbed and released by this magnetic biocomposite. Overall, this work shows that Fe 3 O 4 /anthocyanin magnetic biocomposite has great potential for future applications as a drug storage and delivery nanoplatform that is adaptable to medical, food and sensing.

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“…The unequivocal identity of the metabolites of C3G was recently established from an elegant human feeding study that used penta-13 C-labelled C3G that allowed the source of the metabolites (A-or B-ring of the anthocyanidin) to be established [37,38]. In addition, a number of metabolites of D3G and other trihydroxylated-and methylated-B ring anthocyanins have been reported, although anthocyanin-rich dietary sources were used instead of pure or isotope-labelled compounds [54]. The quantity of the parent (un-metabolized) 13 C-C3G was very low (about 0.147 µm of 13 C-labelled C3G), while the concentration of phenolic metabolites ranged from 0.1 to 2 µM with the cumulative concentration of metabolites reaching 10 µM [38,55] In summary, the data presented in this study do not support the notion that anthocyanins and/or their metabolites significantly affect PON1 gene promoter activity or change the activity of PON1 enzymes.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Anthocyanins and Their Microbial Metabolites on the Expression and Enzyme Activities of Paraoxonase 1, an Important Marker of HDL Function

et al. 2019

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High circulating HDL concentrations and measures of various HDL functions are inversely associated with cardiovascular disease (CVD) risk. Paraoxonase 1 (PON1) contributes to many of the athero-protective functions of HDL, such as promoting the reverse cholesterol transport process and reducing the levels of oxidized LDL. PON1 activities are influenced by several factors, the most important being diet and genetic polymorphisms. Reported data from randomized controlled trials have shown that anthocyanin consumption increased PON1 activity. However, the underlying molecular mechanisms by which anthocyanins increase PON1 activity are not understood. Therefore, the aim of this research was to investigate the ability of anthocyanins and their metabolites to increase PON1 gene expression and/or enzyme activities as potential mechanisms. The effect of the two predominant dietary anthocyanins and 18 of their recently identified microbial metabolites including their phase-II conjugates on PON1 gene expression was studied using a PON1-Huh7 stably-transfected cell line and reporter gene assay. The effects of these compounds on PON1 arylesterase and lactonase activities were investigated using two isoforms of the PON1 enzyme that are the phenotypes of the 192Q/R polymorphism. None of the compounds caused even modest changes in PON1 promoter activity (p ≥ 0.05). Further, none of the compounds at physiological concentrations caused any significant changes in the arylesterase or lactonase activity of either of the iso-enzymes. Cyanidin reduced the lactonase activity of the PON1-R192R enzyme at high concentrations (−22%, p < 0.001), but not at physiologically achievable concentrations. In conclusion, none of the data reported here support the notion that anthocyanins or their metabolites affect PON1 transactivation or enzyme activities.

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Human intervention study to investigate the intestinal accessibility and bioavailability of anthocyanins from bilberries

Cited by 108 publications

References 45 publications

Physiological Concentrations of Blueberry‐Derived Phenolic Acids Reduce Monocyte Adhesion to Human Endothelial Cells

Physiological Concentrations of Blueberry‐Derived Phenolic Acids Reduce Monocyte Adhesion to Human Endothelial Cells

Preparation and pH Controlled Release of Fe3O4/Anthocyanin Magnetic Biocomposites

The Effects of Anthocyanins and Their Microbial Metabolites on the Expression and Enzyme Activities of Paraoxonase 1, an Important Marker of HDL Function

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