Preparation and pH Controlled Release of Fe3O4/Anthocyanin Magnetic Biocomposites

Abstract

Anthocyanins are a class of antioxidants extracted from plants, with a variety of biochemical and pharmacological properties. However, the wide and effective applications of anthocyanins have been limited by their relatively low stability and bioavailability. In order to expand the application of anthocyanins, Fe 3 O 4 /anthocyanin magnetic biocomposite was fabricated for the storage and release of anthocyanin in this work. The magnetic biocomposite of Fe 3 O 4 magnetic nanoparticle-loaded anthocyanin was prepared through physical intermolecular adsorption or covalent cross-linking. Scanning electron microscopy (SEM), Dynamic light scattering (DLS), Fourier-transform infrared spectroscopy (FTIR), X-ray diffractometry (XRD) and thermal analysis were used to characterize the biocomposite. In addition, the anthocyanin releasing experiments were performed. The optimized condition for the Fe 3 O 4 /anthocyanin magnetic biocomposite preparation was determined to be at 60 • C for 20 h in weak alkaline solution. The smooth surface of biocomposite from SEM suggested that anthocyanin was coated on the surface of the Fe 3 O 4 particles successfully. The average size of the Fe 3 O 4 /anthocyanin magnetic biocomposite was about 222 nm. Under acidic conditions, the magnetic biocomposite solids could be repeatable released anthocyanin, with the same chemical structure as the anthocyanin before compounding. Therefore, anthocyanin can be effectively adsorbed and released by this magnetic biocomposite. Overall, this work shows that Fe 3 O 4 /anthocyanin magnetic biocomposite has great potential for future applications as a drug storage and delivery nanoplatform that is adaptable to medical, food and sensing.

Fluorescence Quantum Yields and Lifetimes of Aqueous Natural Dye Extracted from Tradescantia pallida purpurea at Different Hydrogen Potentials

“…The discrete band at 3023 cm −1 and 2926 cm −1 , due to a methyl group (C-H3), can be attributed to aliphatic CH vibrations [55], and with decreasing pH values, peaks at ~3187 cm −1 increased, and other peaks at ~3023 cm −1 and 2926 cm −1 appeared. Similar results were reported for anthocyanin in methanol solution with different pH values, where with the decrease of pH (from 3 to 1), the O-H peak gradually shifted from 3310 cm −1 to 3270 cm −1 due to the hydroxyl groups of methanol changing into the hydroxyl groups of anthocyanin [56]. The C-O group was detected at 1022 cm −1 , and the absorption band in the region of 2950-2840 cm −1 can be attributed to the C-H absorption stretching with the sugar group.…”

Enhancement of Stability and Antioxidant Activity of Mulberry Anthocyanins Through Succinic Acid Acylation

“…The relatively poor chemical stability of anthocyanins in vitro as well as in vivo (in plant cells or in the digestive tracts of animals) is a critical drawback and primary barrier that limits their wide and effective applications, raising up concerns of the importance of reducing their degradation ( 15 , 16 ). To improve the anthocyanin stability, several modification methods have been studied, including glycosyl acylation ( 15 ), glycosylation ( 16 ), microencapsulation ( 17 ), metal chelation ( 18 ), liposomes ( 19 ) and Fe 3 O 4 magnetic nanoparticle ( 20 ). From the nutritional viewpoint, acylated anthocyanins have been reported to possess antioxidant activity ( 21 ).…”