HUMAN INTERFERON AND CELL GROWTH INHIBITION <i>I. Inhibitory Effect of Human Interferon on the Growth Rate of Cultured Human Cells</i>

Interferon was induced by infecting mice with Brucella suis. Serum containing interferon activity was analyzed by chromatography on Concanavalian A-Sepharose and Phenyl-Sepharose CL-4B columns. Antiviral activity was completely retained by the lectin column indicating that all the interferon molecules are glycosylated. The chromatographic behaviour of Brucella interferon on Phenyl-Sepharose CL-4B shows that, like other interferons, Brucella displays hydrophobic properties. However, the hydrophobicity of the interferon molecule was masked in the crude preparation and was only detectable when purified Brucella interferon was used for chromatography. The antigenic properties of Brucella interferon provided the means for developing an affinity chromatographic method resulting in about 60,000 fold purification. As in the case of viral interferon, treatment of L cells with Brucella interferon induced specific enhanced in vitro phosphorylation of a 67,000 molecular weight protein after incubation of cell extracts with double-stranded RNA and [gamma-32P] ATP.

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Human Interferon and Cell Growth Inhibition. VII. Reversibility of Interferon Activities

Dahl¹

1983

Journal of Interferon Research

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Interferon (IFN) components isolated from human leukocyte-derived alpha IFN preparations were tested for reversibility of activities in four different human cell lines. As expected, antiviral activity was not reversed by removal of the IFN-containing medium. Growth inhibition by the anticellular component was completely reversible by washing of the cultures, while that of the antiviral component could not be reversed even after careful washing. The results with the components are in agreement with the findings with IFN in the different cell lines. The present study support earlier suggestions that at least two mechanisms of action are involved in growth regulation by IFN in various cell lines.

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HUMAN INTERFERON AND CELL GROWTH INHIBITION I. Inhibitory Effect of Human Interferon on the Growth Rate of Cultured Human Cells

Abstract: Human interferon inhibited the growth rate of cultured human cells. These included diploid fibroblast cells from embryonal lung and skin, established lines of HeLa and U‐amnion cells, all grown in monolayer;

Cited by 5 publications

References 20 publications

Sensitivity of various human lymphoblastoid cells to the antiviral and anticellular activity of human leukocyte interferon

Sensitivity of various human lymphoblastoid cells to the antiviral and anticellular activity of human leukocyte interferon

Studies on Brucella interferon: Chromatographic behaviour and purification

Human Interferon and Cell Growth Inhibition. VII. Reversibility of Interferon Activities

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