Sensitivity of various human lymphoblastoid cells to the antiviral and anticellular activity of human leukocyte interferon

Hilfenhaus, J.; Damm, H.; Johannsen, R.

doi:10.1007/bf01314795

Cited by 29 publications

(14 citation statements)

References 20 publications

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“…Inhibition of Cell Proliferation. Daudi cells are extremely sensitive to the antiproliferative effects of human IFN-a or -,B (1)(2)(3)(4)(5). Treatment of the cells with 10 units/ml for 24 hr produced a small decrease in cell number (=18%), and by 48 *hr the inhibition of growth was very marked (Fig.…”

mentioning

confidence: 95%

“…The Burkitt lymphoma-derived human lymphoblastoid cell line Daudi is particularly sensitive to the antiproliferative effects of human interferons (1)(2)(3), and inhibition of cell proliferation is accompanied by a large decrease in the ability of the cells to incorporate exogenous [3H]thymidine into DNA (3)(4)(5). The inhibition of cell growth after treatment with naturally occurring or recombinant interferon a (IFN-a) is associated with the differentiation of Daudi cells from an immature B cell phenotype into cells with several characteristics of mature plasma cells (6).…”

mentioning

confidence: 99%

“…Daudi cells were grown at 370C in stationary suspension culture as described previously (2)(3)(4)(5) and were maintained at a density of [1][2][3][4][5][6] (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30) Ci/mmol; 1 Ci = 37 GBq) at 1 gCi/ml for 1 hr.…”

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confidence: 99%

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Induction of B-cell differentiation antigens in interferon- or phorbol ester-treated Daudi cells is impaired by inhibitors of ADP-ribosyltransferase.

Exley¹,

Gordon²,

Clemens³

1987

Proc. Natl. Acad. Sci. U.S.A.

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Treatment of the Daudi Burkitt lymphomaderived cell line with human interferon a, which inhibits cell proliferation in this system, induces differentiation of these B-lymphoid cells into cells with a plasmacytoid phenotype. This differentiation, quantified by the appearance of surface antigens characteristic of mature plasma cells, is impaired by addition to the culture medium of the ADP-ribosyltransferase (ADPRT; EC 2.4.2.30) inhibitors 3-methoxybenzamide or 3-aminobenzamide. These agents also protect the cells against the inhibition of proliferation induced by low doses of interferon a. In contrast, the large inhibition of thymidine incorporation into DNA caused by interferon treatment is not affected by the ADPRT inhibitors. The phorbol ester phorbol 12-tetradecanoate 13-acetate induces the same plasma cell surface antigens that are induced by interferon treatment, and this effect is also impaired by the ADPRT inhibitors. These results suggest that interferons and phorbol esters share a mechanism of action that requires ADPRT activity. Protection of the cells against the antiproliferative effect of interferons by the ADPRT inhibitors suggests that growth inhibition may be a consequence of cell differentiation. In contrast, the inhibition of thymidine incorporation alone is not sufficient for the cessation of cell proliferation and is not a true reflection of the rate of DNA synthesis.

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confidence: 95%

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confidence: 99%

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Induction of B-cell differentiation antigens in interferon- or phorbol ester-treated Daudi cells is impaired by inhibitors of ADP-ribosyltransferase.

Exley¹,

Gordon²,

Clemens³

1987

Proc. Natl. Acad. Sci. U.S.A.

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“…In addition, participation of IDO in host-cell defense mechanisms against proliferation of intracellular parasites following IFN-y treatment has suggested another important biological role for this enzyme (34,35). In light of the known antiproliferative actions of IFNs on either normal or malignant cells (36)(37)(38)(39)(40)(41)(42)(43)(44) and of the requirement of L-tryptophan, the least abundant amino acid, for protein synthesis and cellular growth (45)(46)(47)(48), we investigated whether the induction of IDO constitutes another mechanism to explain the antiproliferative action of IFNs.…”

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confidence: 99%

Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma.

Ozaki

Edelstein

Duch

1988

Proc. Natl. Acad. Sci. U.S.A.

220

134

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The antiproliferative effects of interferon a (IFN-a) and interferon y (IFN-y) were found to be celldependent. Among the human cell lines examined, IFN-y had a greater antiproliferative effect against cell lines that exhibited induction of indoleamine 2,3-dioxygenase, such as the KB oral carcinoma or WiDr colon adenocarcinoma, than against those that lacked the enzyme activity, such as the SW480 colon adenocarcinoma or NCI-H128 small-cell lung carcinoma. MATERIALS AND METHODSCell Culture. Human cell lines HL-60 (promyelocytic leukemia), KG-1 (myelogenous leukemia), CCRF-SB (B-cell leukemia), CCRF-CEM (T-cell, leukemia), MOLT-4 (T-cell leukemia), THP-1 (monocytic leukemia), U-937 (histiocytic lymphoma), NCI-H69 and NCI-H128 (small-cell lung carcinomas), A-427 (lung carcinoma), MRC-5 (normal fetal lung), HEp-2 (larynx carcinoma), KB (oral carcinoma), MCF7 (breast adenocarcinoma), SW480 (colon adenocarcinoma), WiDr (colon adenocarcinoma), and I407 (normal embryonic intestine) were obtained from the American Type Culture Collection. The cells (0.3-1 x 106) were grown in 60-mm plastic culture plates (Costar, Cambridge, MA) containing 6 ml of culture medium consisting of 25 mM Hepes-buffered RPMI 1640 (GIBCO) with 10% heat-inactivated fetal bovine serum, penicillin (50 units/ml), and streptomycin (0.05 mg/ ml; Hazleton Research Products, Lenexa, KS) in the presence of IFN-a

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“…Even though P3HR-1 and Ag-876 expressing EBNA and EA/VCA, and Raji cells expressing only EBNA were not affected by the treatment with the LDV/7 interferon, these virusinfected cells could be destroyed by effector cells activated by interferon (16,17). Since the inhibition of transformation of cord blood cells by Ag-876 EBV was observed in low multiplicity of infection, perhaps more purified and higher doses of interferon may prevent transformation even with higher levels of viruses.…”

Section: Ldvmentioning

confidence: 84%

Spontaneous Production of High Levels of Leukocyte ( ) Interferon by a Human Lymphoblastoid B-Cell Line (LDV/7)

Ablashi

Baron

Armstrong

et al. 1982

Experimental Biology and Medicine

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A human lymphoblastoid B-cell line (LDV/7) capable of replicating significant levels of transfer factor and immune RNA was found to produce high quantities of interferon continuously. The maximum yield of interferon (600 units/ml) was detected on the seventh day. The interferon was characterized as human leukocyte (a) type. This interferon exhibited broad antiviral activity against vesicular stomatitis virus, poliovirus, sindbis virus, Herpesvirus saimiri, and Epstein-Barr virus. Treatment of LDV/7 cells with TPA, PHA, or LPS failed to enhance interferon production. Furthermore, LDV/7 cells could not be induced with Sendai virus to produce larger amounts of interferon. Interferon production correlated best with cell proliferation. It is therefore possible that this cell line may be a good source for large-scale production of human leukocyte interferon as well as for cloning of the interferon genome, and for studies of gene expression.114 0037-9727/82/090 1

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Sensitivity of various human lymphoblastoid cells to the antiviral and anticellular activity of human leukocyte interferon

Cited by 29 publications

References 20 publications

Induction of B-cell differentiation antigens in interferon- or phorbol ester-treated Daudi cells is impaired by inhibitors of ADP-ribosyltransferase.

Induction of B-cell differentiation antigens in interferon- or phorbol ester-treated Daudi cells is impaired by inhibitors of ADP-ribosyltransferase.

Induction of indoleamine 2,3-dioxygenase: a mechanism of the antitumor activity of interferon gamma.

Spontaneous Production of High Levels of Leukocyte ( ) Interferon by a Human Lymphoblastoid B-Cell Line (LDV/7)

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