Human Hormone Production in vitro

Pattillo, Roland A.; Gey, George O.; Delfs, Eleanor; Mattingly, Richard F.

doi:10.1126/science.159.3822.1467

Cited by 125 publications

(79 citation statements)

References 7 publications

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“…Thus, in most cases, choriocarcinoma has the appearance of trophoblast, being predominantly syncytiotrophoblastic or cytotrophoblastic. Some cytotrophoblastic choriocarcinomas secrete little human chorionic gonadotropin and some choriocarcinomas also secrete human placental lactogen [22][23][24][25][26][27]. A number of choriocarcinoma cell lines have been established; these replicating trophoblasts, derived from the malignant tumor or produced by viral transformation of normal trophoblasts, are appropriate systems to mimick trophoblast behaviour in vitro.…”

Section: Discussionmentioning

confidence: 99%

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Trophoblast‐like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins

Wadsack¹,

Hrzenjak²,

Hammer³

et al. 2003

European Journal of Biochemistry

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As human choriocarcinoma cells display many of the biochemical and morphological characteristics reported for in utero invasive trophoblast cells we have studied cholesterol supply from high density lipoproteins (HDL) to these cells. Binding properties of 125I‐labeled HDL subclass 3 (HDL3) at 4 °C were similar for BeWo, JAr, and Jeg3 choriocarcinoma cell lines while degradation rates at 37 °C were highest for BeWo. Calculating the selective cholesteryl ester (CE)‐uptake as the difference between specific cell association of [3H]CE‐labeled HDL3 and holoparticle association of 125I‐labeled HDL3 revealed that in BeWo cells, the selective CE‐uptake was slightly lower than holoparticle association. However, the pronounced capacity for specific cell association of [3H]CE‐HDL3 and selective [3H]CE‐uptake in excess of HDL3–holoparticle association, and cAMP–mediated enhanced cell association of [3H]CE‐HDL3 in JAr and Jeg3 suggested the scavenger receptor class B, type I (SR‐BI) to be responsible for this pathway. Abundant expression of SR‐BI (but not SR‐BII, a splice variant of SR‐BI) could be observed in JAr and Jeg3 but not in BeWo cells using RT‐PCR, Northern and Western blot analysis, and immunocytochemical technique. Adenovirus‐mediated overexpression of SR‐BI in all three choriocarcinoma cell lines resulted in an enhanced capacity for cell association of [3H]CE‐HDL3 (20‐fold in BeWo; fivefold in JAr and Jeg3). The fact that exogenous HDL3 remarkably increases proliferation in JAr and Jeg3 supports the notion that selective CE‐uptake and subsequent intracellular generation of cholesterol is coupled to cellular growth. From our findings we propose that JAr and Jeg3 cells serve as a suitable in vitro model to study selective CE‐supply to human placental cells.

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Section: Discussionmentioning

confidence: 99%

“…BeWo, JAr, and Jeg3. The BeWo cell line which is heterogenous on several criteria [22], is comprised of cytotrophoblasts with no differentiation to syncytium [23] under non activated conditions [24]. This is in contrast to primary cultures of term cytotrophoblasts which aggregate and form syncytia.…”

mentioning

confidence: 99%

Trophoblast‐like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins

Wadsack¹,

Hrzenjak²,

Hammer³

et al. 2003

European Journal of Biochemistry

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“…Among these are naturally immortalized cell lines obtained from choriocarcinoma tissue, such as BeWo [16], JAR [17] and JEG-3 [18]. The cell lines AC1M-32 [19] and ACH-3P [20] have been generated by fusion of the AC1-1 cell line (a JEG-3 mutant [21]) with primary term or first trimester trophoblasts, respectively.…”

Section: Introductionmentioning

confidence: 99%

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

et al. 2015

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“…BeWo cells are derived from a malignant choriocarcinoma (23), and thus may have a number of differences when compared to normal cytotrophoblasts and syncytiotrophoblasts. Therefore, we used primary cultures of trophoblasts, obtained from placentas harvested after normal-term pregnancies, to examine E-cadherin expression and the role of InlA in bacterial attachment and entry.…”

Section: Inla Mediates Bacterial Invasion Of Primary Syncytiotrophoblmentioning

confidence: 99%

Targeting and crossing of the human maternofetal barrier byListeria monocytogenes: Role of internalin interaction with trophoblast E-cadherin

Lecuit

Nelson

Smith

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

213

183

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Listeria monocytogenes produces severe fetoplacental infections in humans. How it targets and crosses the maternofetal barrier is unknown. We used immunohistochemistry to examine the location of L. monocytogenes in placental and amniotic tissue samples obtained from women with fetoplacental listeriosis. The results raised the possibility that L. monocytogenes crosses the maternofetal barrier through the villous syncytiotrophoblast, with secondary infection occurring via the amniotic epithelium. Because epidemiological studies indicate that the bacterial surface protein, internalin (InlA), may play a role in human fetoplacental listeriosis, we investigated the cellular patterns of expression of its host receptor, E-cadherin, at the maternofetal interface. E-cadherin was found on the basal and apical plasma membranes of syncytiotrophoblasts and in villous cytotrophoblasts. Established trophoblastic cell lines, primary trophoblast cultures, and placental villous explants were each exposed to isogenic InlA؉ or InlA؊ strains of L. monocytogenes, and to L. innocua expressing or not InlA. Quantitative assays of cellular invasion demonstrated that bacterial entry into syncytiotrophoblasts occurs via the apical membrane in an InlA-E-cadherin dependent manner. In human placental villous explants, bacterial invasion of the syncytiotrophoblast barrier and underlying villous tissue and subsequent replication produces histopathological lesions that mimic those seen in placentas of women with listeriosis. Thus, the InlA-E-cadherin interaction that plays a key role in the crossing of the intestinal barrier in humans is also exploited by L. monocytogenes to target and cross the placental barrier. Such a ligand-receptor interaction allowing a pathogen to specifically cross the placental villous trophoblast barrier has not been reported previously.

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Human Hormone Production in vitro

Cited by 125 publications

References 7 publications

Trophoblast‐like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins

Trophoblast‐like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins

Toll-like receptor profiling of seven trophoblast cell lines warrants caution for translation to primary trophoblasts

Targeting and crossing of the human maternofetal barrier byListeria monocytogenes: Role of internalin interaction with trophoblast E-cadherin

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