“…For ITC, we have demonstrated how this can substantially improve the level of detail and reveal the cooperativity parameters in ternary multiprotein complexes (Houtman et al, 2007); in sedimentation equilibrium analytical ultracentrifugation (SE) SEDPHAT has been routinely used in global analysis to overcome the limitation of ill-conditioned SE data and obtain better determination of thermodynamic parameters (Vistica et al, 2004;Ghirlando, 2011;Zhao, Brautigam et al, 2013); and multiple approaches for global analysis in sedimentation velocity analytical ultracentrifugation (SV) include direct boundary modeling with Lamm equation solutions for determining equilibrium and kinetic binding constants, as well as hydrodynamic shape parameters (Schuck, 2003;Dam et al, 2005;Brautigam, 2011), multi-signal analysis (MSSV) for determining the number and composition of co-existing complexes (Balbo et al, 2005; and global density-contrast analysis for determining macromolecular partial specific volume (Brown et al, 2011). Finally, applications of global analysis of combined SE and SV data in SEDPHAT have demonstrated the utility of a more generalized sedimentation analysis (Canzio et al, 2013;May et al, 2014).…”