Human group IIA secretory phospholipase A₂ potentiates Ca²⁺ influx through L‐type voltage‐sensitive Ca²⁺ channels in cultured rat cortical neurons

Induction of secretory phospholipase A2 in reactive astrocytes in response to transient focal cerebral ischemia in the rat brain

“…sPLA 2 purified from taipan venom was shown to bind neural membranes with high affinity and elicit neurotoxicity (Lambeau et al 1989). More recently, human sPLA 2 -IIA was also shown to pontentiate Ca 2+ influx through L-type voltage-sensitive Ca 2+ channels in neurons (Yagami et al 2003). Indeed, there is strong evidence for sPLA 2 -enhanced glutamate signaling leading to neuronal cell death (Kolko et al 1996(Kolko et al , 1999DeCoster et al 2002;Rodriguez de Turco et al 2002).…”

Secretory Phospholipases A2 Induce Neurite Outgrowth in PC12 Cells through Lysophosphatidylcholine Generation and Activation of G2A Receptor

“…The secreted type of PLA 2 , sPLA 2 , is a small (13)(14)(15)(16)(17)(18)(19)(20), Ca 2ϩ -dependent, disulfide-rich protein composed of extremely diverse members present in venoms, digestive exudates, inflammation sites, various mammalian tissues, and in microorganisms. In mammals, 11 genes encoding distinct sPLA 2 isozymes that display overlapping yet distinct tissue distributions have been identified through the extensive genomic search, but the precise roles for each individual isozyme largely remain to be specified.…”

Anti-heat Shock 70 kDa Protein Antibody Induced Neuronal Cell Death

“…41) sPLA 2 enhances ROS production 43) and stimulates oxidative signaling pathways in the stroke brain. 44) 15d-PGJ 2 increases ROS at neurotoxic concentrations. 21) Similarly, anti-Hsp70 antibody generated ROS in a concentrationdependent manner.…”