Anti-heat Shock 70 kDa Protein Antibody Induced Neuronal Cell Death

Abstract

Heat shock proteins (Hsps) are stress-induced proteins that are involved in cellular protection and repair mechanisms.

15-Deoxy-Δ^12,14-prostaglandin J₂ Inhibits Cell Migration on Renal Cell Carcinoma <i>via</i> Down-Regulation of Focal Adhesion Kinase Signaling

“…The Cytotoxic Effect of 15d-PGJ 2 on Caki-2 Cells 15d-PGJ 2 induces apoptosis in a several of cancer cell lines. 20) To confirm non-cytotoxic concentrations of 15d-PGJ 2 , cytotoxicity was assessed by MTT assay in the present study. The cytotoxic effect of 15d-PGJ 2 was examined on human RCC cell line, Caki-2.…”

“…13,14,24) The down-regulation of PI3K-Akt signaling has been reported to mediate the inhibition of metastasis in RCC, 21) suggesting that 15d-PGJ 2 might inhibit the metastasis of Caki-2 cells via the suppression of PI3K-Akt signaling. In addition, topoisomerase inhibitors such as camptothecine, 19) etoposide 20) and doxorubicin 13) enhanced the 15d-PGJ 2 -induced apoptosis. Although their enhancements were also independent of PPARγ, they did not enhance the inhibitory effect of 15d-PGJ 2 on the migration of Caki-2 cell (data not shown).…”

“…[15][16][17][18] We also confirmed the synergistic effects of 15d-PGJ 2 on the cytotoxicity with topoisomerase-I inhibitor 19) and topoisomerase-II inhibitors. 14,20) 15d-PGJ 2 has also been reported to reduce cell migration, stimulate focal adhesion disaggregation, and induce filamentous actin (F-actin) realignment at low concentrations, independent of PPARγ. 5) Interestingly, the p38 mitogen-activated protein (MAP) kinase inhibitor prevented both 15d-PGJ 2 -induced F-actin realignment and focal adhesion disaggregation.…”

4,4-Diisothiocyanatostilbene Disulfonic Acid Enhanced 15-Deoxy-Δ^12,14-prostaglandin J₂-Induced Neuronal Apoptosis

“…VDAC has been reported as one of candidate proteins targeted for 15d-PGJ 2 by the proteomic approach. 21) 15d-PGJ 2 condensed chromatin, activated caspase-3 and induced neuronal apoptosis. In addition, 15d-PGJ 2 incorporated PI into neurons, indicating disruption of plasma membrane.…”

A plausible involvement of plasmalemmal voltage‐dependent anion channel 1 in the neurotoxicity of 15‐deoxy‐Δ12,14‐prostaglandin J2

“…Besides CRTH2, we have reported neuron‐specific enolase (Yamamoto et al., 2015a), 14‐3‐3ξ (Yamamoto et al., 2015b), and heat shock protein 70 (Yamamoto et al., 2017) among membrane targets for 15d‐PGJ 2 (molecular weights more than 33 kDa, PI values less than 7) (Yamamoto et al., 2011). We are searching for evidences that 15d‐PGJ 2 caused neuronal apoptosis via the above three targets.…”