Human Group 1 Innate Lymphocytes Are Negative for Surface CD3ε but Express CD5

Roan, Florence; Ziegler, Steven F.

doi:10.1016/j.immuni.2017.04.024

Cited by 18 publications

(19 citation statements)

References 8 publications

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“…Recently CD4 + ILC1 were found in peripheral blood ( 41 ). We confirmed those findings and found that those CD4 + ILC1 also expressed CD5 [( 42 ) and Nagasawa, unpublished observation]. However, in contrast, the cord blood and thymic CD5 + ILCs described here, which express CD161, lacked CD4.…”

Section: Discussionsupporting

confidence: 90%

Human CD5+ Innate Lymphoid Cells Are Functionally Immature and Their Development from CD34+ Progenitor Cells Is Regulated by Id2

et al. 2017

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Innate lymphoid cells (ILCs) have emerged as a key cell type involved in surveillance and maintenance of mucosal tissues. Mouse ILCs rely on the transcriptional regulator Inhibitor of DNA-binding protein 2 (Id2) for their development. Here, we show that Id2 also drives development of human ILC because forced expression of Id2 in human thymic progenitors blocked T cell commitment, upregulated CD161 and promyelocytic leukemia zinc finger (PLZF), and maintained CD127 expression, markers that are characteristic for human ILCs. Surprisingly CD5 was also expressed on these in vitro generated ILCs. This was not an in vitro artifact because CD5 was also found on ex vivo isolated ILCs from thymus and from umbilical cord blood. CD5 was also expressed on small proportions of ILC2 and ILC3. CD5+ ILCs were functionally immature, but could further differentiate into mature CD5− cytokine-secreting ILCs. Our data show that Id2 governs human ILC development from thymic progenitor cells toward immature CD5+ ILCs.

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Section: Discussionsupporting

confidence: 90%

Human CD5+ Innate Lymphoid Cells Are Functionally Immature and Their Development from CD34+ Progenitor Cells Is Regulated by Id2

et al. 2017

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“…As suggested, these cells could represent a heterogeneous population of innate‐like T lymphocytes or T cell‐like innate lymphocytes. However, this issue is not completely resolved …”

Section: Ilc Subset Heterogeneity In Humansmentioning

confidence: 98%

“…However, this issue is not completely resolved. [28][29][30] Group 2 ILC Group 2 ILCs are characterized by cells capable of producing of Th2-like cytokines (e.g. IL-5, IL-13) with high expression of the transcription factor GATA binding protein 3 (GATA3).…”

Section: Ilc Subset Heterogeneity In Humansmentioning

confidence: 99%

Dissecting human ILC heterogeneity: more than just three subsets

Simoni

Newell

2017

Immunology

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SummaryInnate lymphoid cells (ILCs) have been divided into three distinct groups based on functional capacities, cytokine profiles and transcription factor expression. Studies performed mainly in mice have demonstrated the importance of ILCs in chronic inflammation, infection, allergy and cancer. In this review, we discuss the heterogeneity of human ILC and focus primarily on the taxonomy of human ILC cell subsets and their phenotypical and functional diversity. We summarize recent findings concerning the diversity of ILCs between and within the major subsets [natural killer (NK), ILC1, intra-epithelial ILC1 (ieILC1), ILC2, ILC3, lymphoid tissues inducer (LTi) and ILC progenitor (ILCP)], as well as the abundance of each in human tissues. We also discuss the similarities observed between groups of cells in term of receptors expressed and cytokines produced, and how these relate to the pleiotropic properties of each subset.

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“…Beyond the technical details underlying the failure to reveal T-BET + ILC1 in human tissues and the limitations of this approach [explained in Ref. ( 133 )], the definition of human type 1 ILC populations remains a subject of debate, due to the absence of specific markers and the expression of molecules associated to T cell biology, including intracellular expression of CD3 and surface expression of CD5 ( 134 , 135 ). Despite the increasing awareness of ILC heterogeneity, it will be important to clarify to what extent the degree of complexity defines truly distinct ILC subpopulations, with different functions, or whether reflects a range of activation states within a single population.…”

Section: Recent Advances From Single-cell Analysesmentioning

confidence: 99%

Epigenomic Views of Innate Lymphoid Cells

et al. 2017

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The discovery of innate lymphoid cells (ILCs) with selective production of cytokines typically attributed to subsets of T helper cells forces immunologists to reassess the mechanisms by which selective effector functions arise. The parallelism between ILCs and T cells extends beyond these two cell types and comprises other innate-like T lymphocytes. Beyond the recognition of specialized effector functionalities in diverse lymphocytes, features typical of T cells, such as plasticity and memory, are also relevant for innate lymphocytes. Herein, we review what we have learned in terms of the molecular mechanisms underlying these shared functions, focusing on insights provided by next generation sequencing technologies. We review data on the role of lineage-defining- and signal-dependent transcription factors (TFs). ILC regulomes emerge developmentally whereas the much of the open chromatin regions of T cells are generated acutely, in an activation-dependent manner. And yet, these regions of open chromatin in T cells and ILCs have remarkable overlaps, suggesting that though accessibility is acquired by distinct modes, the end result is that convergent signaling pathways may be involved. Although much is left to be learned, substantial progress has been made in understanding how TFs and epigenomic status contribute to ILC biology in terms of differentiation, specification, and plasticity.

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Human Group 1 Innate Lymphocytes Are Negative for Surface CD3ε but Express CD5

Cited by 18 publications

References 8 publications

Human CD5+ Innate Lymphoid Cells Are Functionally Immature and Their Development from CD34+ Progenitor Cells Is Regulated by Id2

Human CD5+ Innate Lymphoid Cells Are Functionally Immature and Their Development from CD34+ Progenitor Cells Is Regulated by Id2

Dissecting human ILC heterogeneity: more than just three subsets

Epigenomic Views of Innate Lymphoid Cells

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