Human gross cyst breast disease and cystic fluid: bio-molecular, morphological, and clinical studies

Mannello, Ferdinando; Tonti, Gaetana A.; Papa, Stefano

doi:10.1007/s10549-005-9110-y

Cited by 42 publications

(94 citation statements)

References 177 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Desde então, outros trabalhos foram surgindo com a evolução das técnicas moleculares e diversas proteínas foram sendo apontadas ao longo dos anos como sugestivas de biomarcadores para o câncer de mama, como exemplo, a prolactin inducible protein e a gross cystic disease fluid protein. 37 Nos últimos anos o NAF tem ganhado destaque novamente pela introdução da biópsia líquida e o avanço das tecnologias aplicadas à proteômica. A coleta do fluido, justamente por ser de baixo custo, tem despertado o interesse na clínica para investigação; porém, a coleta ainda é dolorosa e feita por meio de bombas a vácuo, que geram quantidades mínimas do fluido para pesquisa laboratorial.…”

Section: Salivaunclassified

O presente e o futuro dos marcadores circulantes de câncer

Alves

Delmonico

2015

Revista Hospital Universitário Pedro Ernesto

View full text Add to dashboard Cite

Artigo de revisão ResumoPara alguns tipos de câncer, existem marcadores circulantes em uso que não apresentam especificidade e sensibilidade suficientes. Por isso, novas tecnologias e novos conhecimentos estão emergindo com o objetivo de encontrar os marcadores circulantes ideais para cada subtipo de câncer. Recentemente importantes avanços na pesquisa clínica permitiram a identificação de DNA, RNA, proteínas livres no plasma, na saliva, em descarga mamilar e na urina como potenciais e promissores marcadores para o câncer. Outra possível fonte de marcadores circulantes são as vesículas extracelulares, que têm um papel importante na comunicação celular. Em vista das novas descobertas, algumas das quais reunidas nesta revisão de literatura, os conceitos tradicionais de metástase e de micrometástase merecem ser revistos.Descritores: Metástase neoplásica; Diagnóstico; Plasma; Mama; Saliva; Urina. Abstract The present and the future of circulating cancer markersFor some types of cancer, there are circulating markers in use that don't have sufficient specificity and sensitivity. New technologies and new knowledge are thus emerging in order to find the ideal circulating markers for each cancer subtype. Important recent advances in clinical research have allowed for the identification of free circulating DNA, RNA, and proteins from plasma, saliva, nipple aspirate fluid, and urine as potential and promising circulating markers of cancer. Another possible source of circulating markers are the extracellular vesicles, which play an important role in cell communication. Considering new discoveries, some of which are presented in this review, the traditional models of metastasis and micrometastasis deserve to be reviewed.Keywords: Neoplasm metastasis; Diagnosis; Plasma; Saliva; Breast; Urine. O presente e o futuro dos marcadores circulantes de câncer Resumen El presente y el futuro de los marcadores circulantes de cáncerPara algunos tipos de cáncer, hay marcadores circulantes en uso que tienen especificidad y sensibilidad insuficiente. Por lo tanto, las nuevas tecnologías y los nuevos conocimientos surgen con el fin de encontrar los marcadores circulantes ideales para cada subtipo de cáncer. Recientemente, los avances significativos en la investigación clínica llevaron a la identificatión de DNA, RNA y proteínas libres circulantes en plasma, saliva, secreción del pezón, y orina como posibles y prometedores marcadores de cáncer. Otra posible fuente de marcadores circulantes son las vesículas extracelulares, que desempeñan un papel importante en la comunicación celular. Teniendo en cuenta los nuevos descubrimientos, algunos de los que se ajuntan a esta revisión de la literatura; los modelos tradicionales de metástasis y micrometástasis merecen ser revisados.

show abstract

Section: Salivaunclassified

O presente e o futuro dos marcadores circulantes de câncer

Alves

Delmonico

2015

Revista Hospital Universitário Pedro Ernesto

View full text Add to dashboard Cite

show abstract

“…[2] Several large studies have shown that the risk of breast cancer in women with GCBD to be 1.7 to 7.5 times higher, and women with an apocrine breast cyst may have a higher risk of developing breast cancer than women with breast cyst lined by flattened epithelium. [3][4][5] Histological risk factors for breast cancer have been also reported to be more common with type I cysts. [3] A variety of cancer bearing patients have been shown to have disturbances in carbohydrate, lipid and protein metabolisms.…”

Section: Introductionmentioning

confidence: 99%

Breast Cyst Fluid Free Amino Acid Profile

Dağlar¹,

Erbas²,

Gülen³

2009

TUTFD

View full text Add to dashboard Cite

© Trakya Üniversitesi T p Fakültesi Dergisi. AVES Yay nc l k taraf ndan bas lm t r. Her hakk sakl d r. © Medical Journal of Trakya University. Published by AVES Publishing. All rights reserved. Breast Cyst Fluid Free Amino Acid ProfileMeme Kist S v Objective: Gross cystic breast disease (GCBD) is the most common benign breast disease. There are two types of breast cyst; lined by apocrine epithelium (Na/ K<3) or flattened epithelium (Na/K>3). Several studies have shown that women with palpable breast cysts may have 1.7-7.5 times higher risk of developing breast cancer. Patients with malignant disease usually show abnormal amino acid profiles in the peripheral circulation. Changes in amino acid profile diagnostically correlate with organ sites of malignancy. The aim of this study was to investigate the levels of amino acids in two cyst groups and possible mechanisms involved in the development of breast cancer. Material and Methods:The breast cyst fluid aspirated from women with GCBD were analysed. Breast cyst fluid amino acid levels were determined with HPLC.Results: Aspartic acid, glutamic acid, hydroxyproline, serine, glycine, threonine, alanine, proline, tyrosine, methionine, isoleucine, leucine, phenylalanine and tryptophan levels were significantly higher, and lysine levels were lower in the apocrine epithelial cysts. Conclusion:In this study, higher concentrations of amino acids in apocrine cysts, which are also known to have a higher risk of developing breast cancer, may indicate the possible role(s) of amino acids in the mechanism of breast cancer development.Key words: Breast cyst fluid; gross cystic breast disease (GCBD); breast cancer; free amino acids; HPLC.Amaç: Kistik meme hastal klar kad nlarda en fazla görülen meme hastal d r. Apokrin epitelli (Na/K<3) ve düz epitelli (Na/K>3) olmak üzere iki tip meme kisti bulunmaktad r. Yap lan çe itli çal malar memesinde kistik bir olu um bulunan kad nlar n 1.7-7.5 kat daha fazla meme kanserine yakalanma riski ta d klar n ortaya koymu tur. Kanserli hastalar n periferal dola m na bak ld nda ise genellikle anormal bir amino asit profili saptanm t r. Ayr ca, amino asit profilindeki de i iklikler organ düzeyindeki kanserler ile ili kili bulunmu tur. Bu çal man n amac meme kanseri geli imi yönünden yüksek ve dü ük risk grubu kistlerdeki amino asit düzey-lerini incelemek ve bu kistlerden meme kanseri geli imi yönündeki olas mekanizmalar ara t rmakt r. Gereç ve Yöntemler:Çal mada kistik meme hastal olan kad nlardan al nan meme kist s v s kullan ld . Meme kist s v s amino asit düzeyleri HPLC metodu ile ölçüldü. Bulgular: Apokrin epitelli kist grubunda aspartik asit, glutamik asit, hidroksiprolin, serin, glisin, treonin, alanin, prolin, tirozin, metiyonin, izolösin, fenilalanin ve triptofan düzeyleri anlaml olarak yüksek, lizin düzeyi ise dü ük bulundu.Sonuç: Kanser geli imi yönünden yüksek riske sahip olan apokrin epitelli kistlerde bulunan daha yüksek amino asit düzeyleri, amino asitlerin meme kanseri geli im sürecinde potansiyel bir role sahip olabilece ini gösterm...

show abstract

“…These gross cyst types also differ in their natural history, particularly in terms of multiplicity and recurrence [11, 12]. Although care should be taken in translating the potential biological activity of several constituents (e.g., mitogenic growth factors, steroid hormones, proteolytic enzymes) into trophic effects within the breast and subsequent BC risk, gross cysts lined by apocrine epithelium show the ability to actively synthesize and secrete bioactive molecules [5], strengthening the epidemiological evidence of the increased risk for BC in GCBD-affected women [12].…”

mentioning

confidence: 99%

“…Most clinicians favor a policy of cautious/ close observation [20,21], even though there is no certain evidence that the risk for BC in GCBD will fall as a result. We are far from the certainty that biomolecular and morphological analyses of fluid that is needle aspirated from gross cysts will ever be an accurate predictor of BC risk [5], and more studies are needed to answer the question of whether GCBD (and/or associated hyperplastic lesions) increase the risk for BC and to stratify women with GCBD into high-risk groups for BC [9].…”

mentioning

confidence: 99%

“…Although the GCBD does not represent a preneoplastic condition per se [18], it has a peculiar place among BBDs and many stud- Mannello, Tonti 1133 www.TheOncologist.com ies point out that the different gross cyst subtypes differ in epidemiological, biomolecular, and clinical behavior [5,9,11,12,16,19]. Most clinicians favor a policy of cautious/ close observation [20,21], even though there is no certain evidence that the risk for BC in GCBD will fall as a result.…”

mentioning

confidence: 99%

See 1 more Smart Citation

In Reply

Güray

Şahin

2006

The Oncologist

View full text Add to dashboard Cite

We read with concern the review by Guray and Sahin [1] that adds to the body of evidence on clinical features and practical management of benign breast diseases (BBDs) [2]. We believe that the aspects regarding fibrocystic changes contained in the article deserve some comments (in particular for cystic lesions), and furthermore, several caveats are implicit (the data tend to be correlative rather than mechanistic in nature).Although the most common presenting symptoms of fibrocystic changes (FCCs) are breast pain and tender nodularities in the breast, most breast cysts (especially microcysts) are not painful, not progressive, and spontaneously regress. It is therefore possible for women to live their lives in blissful ignorance of the fact that they have a cyst: these women would be those who do not examine their breasts and do not have a heightened awareness of breast cancer (BC). By contrast, the women who discover palpable macrocysts in the breast are those who may practice self-examination and are BC aware [3]. These two groups of women are likely to be very different in terms of other risk factors, and potential epidemiological differences (e.g., a family history of BC, postponement of the first pregnancy, use of the pill, and use of hormone-replacement therapy). These determinants lead to the pathogenesis of BC in women with a history of gross cystic breast disease (GCBD) [4].Moreover, an overview of GCBD studies carried out during the last 30 years sheds light on the different biochemical, molecular, and morphological differences of the two breast gross cyst types [5], providing evidence that the peculiar electrolyte, protein, sex hormone, growth factor, and proteolytic enzyme accumulation reflects both the biochemical environment and the morphological aspect of the epithelium lining the gross cysts, developing into the terminal duct-lobular unit. The different biocompound profiles found in the flattened low-risk and apocrine high-risk gross cysts may aid in the classification of gross cyst subtypes [6] and the identification of a biomolecular/morphological pattern [4,5,7,8] in women with a history of GCBD who go on to develop subsequent breast cancer [9][10][11]. These gross cyst types also differ in their natural history, particularly in terms of multiplicity and recurrence [11, 12]. Although care should be taken in translating the potential biological activity of several constituents (e.g., mitogenic growth factors, steroid hormones, proteolytic enzymes) into trophic effects within the breast and subsequent BC risk, gross cysts lined by apocrine epithelium show the ability to actively synthesize and secrete bioactive molecules [5], strengthening the epidemiological evidence of the increased risk for BC in GCBD-affected women [12].The best evaluation of FCCs should take into account the natural history of some BBDs [4,10], and cytomorphology may be helpful to discern between nonproliferative and proliferative lesions, with and without atypia, through an accurate cytology index [13]. In this respect, s...

show abstract

Human gross cyst breast disease and cystic fluid: bio-molecular, morphological, and clinical studies

Cited by 42 publications

References 177 publications

O presente e o futuro dos marcadores circulantes de câncer

O presente e o futuro dos marcadores circulantes de câncer

Breast Cyst Fluid Free Amino Acid Profile

In Reply

Contact Info

Product

Resources

About