Human granulosa contain messenger ribonucleic acids encoding insulin-like growth factor-binding proteins (IGFBPs) and secrete IGFBPs in culture

Giudice, Linda C.; Milki, Amin A.; Milkowski, Deborah A.; Danasouri, Imam El

doi:10.1016/s0015-0282(16)54543-9

Cited by 44 publications

(8 citation statements)

References 25 publications

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“…It has been suggested that IGFs stimulate follicular growth, dif ferentiation and steroidogenesis. IGF-1 has been reported to stimulate aromatase mRNA levels and P450arom activi ty in cultured human granulosa-luteal cells [20], A family of binding proteins (IGFBP-1-6) which binds with both IGF-I and II with high affinity has been reported to be present in serum [10], amniotic fluid [5,6], other body fluids [7][8][9]11], and in the conditioning medium from several cultured cell lines [21]. It has been suggested that the IGFBPs modulate the availability of the IGF to their target cells, and play an important role in the regulation of autocrine or paracrine action of IGF.…”

Section: Discussionmentioning

confidence: 99%

“…It has been suggested that the IGFBPs modulate the availability of the IGF to their target cells, and play an important role in the regulation of autocrine or paracrine action of IGF. mRNA expression and the production of IGFBP-1 have been observed in human granulosa cells [21,22] and endometrial cells [23], and they have been shown to be regulated via protein kinase C and adenylate cyclase pathways [22], Marrs et al [ 14] microscopically evaluated oocyte mat uration after oocyte retrieval during an IVF-ET program. However, no biochemical marker in the classification of oocyte maturation has so far been found except for ovari an steroid hormones.…”

Section: Discussionmentioning

confidence: 99%

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Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Kawano¹,

Narahara²,

Matsui³

et al. 1997

Gynecol Obstet Invest

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In order to investigate the role of insulin-like growth factors (IGFs) in human ovulation, we evaluated the concentrations of IGF-binding proteins (IGFBPs) in human follicular fluid (FF). The concentrations of IGFBP-1 in the FFs of 15 women undergoing in vitro fertilization and embryo transfer were measured and related to those of 17Β-estradiol (E₂), progesterone and androstenedione in the FFs. IGFBP-1 levels in the FFs were positively correlated with those of E₂ and progesterone. No correlation was found between the IGFBP-1 and androstenedione levels in FFs. The concentrations of IGFBP-1 were significantly increased in the FFs which contained mature oocytes compared with those of immature oocytes, whereas IGFBP-3 in FFs tended to decrease as oocytes matured. It is suggested that IGFs may play important roles in human preovulatory processes, and that IGFBP-1 may be a valuable biochemical marker in the evaluation of oocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Kawano¹,

Narahara²,

Matsui³

et al. 1997

Gynecol Obstet Invest

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“…In contrast, GRFi increased levels of IGFBP-2, -5, and -4 in FFL of SUB but not DOM, suggesting that regulation of these IGFBP in DOM may be occurring locally at the tissue level. Ovarian tissue mRNA for IGFBP-2 through -5 has been identified in the rat [73,741, for IGFBP-2 and -3 in the pig [75], and for IGFBP-1 through -3 in the human [76]. Furthermore, cultured granulosa and thecal cells have been shown to synthesize IGFBP [76][77][78]62].…”

Section: Discussionmentioning

confidence: 99%

“…Ovarian tissue mRNA for IGFBP-2 through -5 has been identified in the rat [73,741, for IGFBP-2 and -3 in the pig [75], and for IGFBP-1 through -3 in the human [76]. Furthermore, cultured granulosa and thecal cells have been shown to synthesize IGFBP [76][77][78]62]. Therefore, changes in local production of IGFBP have the potential to play a key role in regulating the availability of IGF to ovarian cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Somatotropin and/or Equine Chorionic Gonadotropin on Serum and Follicular Insulin-Like Growth Factor I and Insulin-Like Growth Factor Binding Proteins in Cattle1

Stanko

Cohick

Shaw

et al. 1994

Biology of Reproduction

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We investigated the effect of administration of somatotropin (ST) and/or eCG on insulin-like growth factor I (IGF-I) and IGF-binding proteins (IGFBP) in serum and follicular fluid (FFL) of cattle actively immunized against growth hormone-releasing factor (GRF). Cyclic beef cattle, previously immunized against GRF-(1-29)-Gly-Gly-Cys-NH2 conjugated to human serum albumin (synthesized and provided by Hoffmann-LaRoche, Inc., Nutley, NJ; GRFi, n = 31) or to human serum albumin alone (HSAi, n = 26), received (i.m.): 1) 25 mg recombinantly derived methionyl somatotropin (rbST, n = 14; sometribove provided by Monsanto Co., St. Louis, MO); 2) 1100 IU eCG (n = 10); 3) rbST and eCG (rbST-eCG, n = 15); or 4) vehicle (VEH, n = 17) at 0 and 24 h after receiving prostaglandin F2 alpha (PGF2 alpha). Serum samples were collected at 0 and 40 h after PGF2 alpha, and the ovary bearing the largest follicle (DOM) was removed 44.0 +/- 0.5 h after PGF2 alpha; FFL was harvested from DOM and the subordinate follicle (SUB). Before treatment (0 h), GRFi cows had lower serum ST (0.6 +/- 0.2 vs. 2.2 +/- 0.2 ng/ml; p < 0.01) and IGF-I (26 +/- 4 vs. 72 +/- 4 ng/ml; p < 0.01), but greater IGFBP-2 (594 +/- 48 vs. 384 +/- 52 ng/ml; p < 0.01) than HSAi cows. Serum and FFL concentrations of IGF-I or IGFBP-2 were not different between rbST- and rbST-eCG-treated cows or between VEH- and eCG-treated cows at Hour 40 after the initial treatment injection; therefore, data were combined and designated as rbST and VEH, respectively. Serum IGF-I was increased to a greater extent (percentage increase above 0 h) by rbST treatment in GRFi (362 +/- 24) than in HSAi (176 +/- 16) cows (immunization by treatment, p < 0.01). Across GRFi and HSAi, rbST lowered serum IGFBP-2 (342 +/- 31 vs. 541 +/- 27 ng/ml, rbST vs. VEH; p < 0.01). Diameters of DOM or SUB were not affected by immunization or treatment. Concentrations of IGF-I and IGFBP-3 (determined by ligand blot analysis) in FFL from both DOM and SUB were lower (p < 0.05) in GRFi than in HSAi cows. In contrast, IGFBP-2 in FFL was elevated in SUB, but not DOM, in GRFi compared to HSAi cows.(ABSTRACT TRUNCATED AT 400 WORDS)

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“…Upregulation of IGFBP‐2 expression after infusion of IGF [4]and in patients suffering from IGF‐II secreting tumors [5]or in transgenic mice overexpressing IGF‐II [6, 7]suggested a positive regulation of IGFBP‐2 expression by increased levels of free IGFs. IGFBP‐2 might also play a major role in reproductive tissues [8–10], where its decrease was correlated with increased tumorigenicity [11].…”

Section: Introductionmentioning

confidence: 99%

Insulin‐like growth factor‐binding protein‐2 inhibits proliferation of human embryonic kidney fibroblasts and of IGF‐responsive colon carcinoma cell lines

et al. 1998

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So far, the physiological role of insulin-like growth factor binding protein-2 (IGFBP-2) has not been demonstrated directly. Therefore, we transfected 293 cells with an expression vector containing the CMV promoter and the complete cDNA of mouse IGFBP-2. Secretion of bioactive IGFBP-2 into conditioned medium was demonstrated by Western ligand and Western immunoblotting and quantified by specific RIA. For the analysis of cell proliferation three clones exhibiting either high or low/no IGFBP-2 expression were selected and compared to non-transfected parental 293 cells. IGFBP-2 secreting clones displayed reduced conversion of thiazolyl blue when compared to negative clones or non-transfected parental 293 cells (P 6 6 0.01). The lower growth activity measured in the IGFBP-2 secreting clones was compensated in great part by the administration of exogenous IGF-I or -II. Conditioned media of IGFBP-2 secreting clones inhibited growth of IGF-responsive colon tumor cell lines (LS513, HT-29) while those of negative clones did not. In addition, conditioned medium from a clone expressing high levels of IGFBP-2 inhibited anchorage-independent growth of LS513 and HT-29 cells. In contrast, growth of an IGFunresponsive tumor cell line (Co-115) was not affected by the conditioned media. We hypothesize that IGFBP-2 might sequester the IGFs and thus prevent them from transferring their mitogenic signals.z 1998 Federation of European Biochemical Societies.

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Human granulosa contain messenger ribonucleic acids encoding insulin-like growth factor-binding proteins (IGFBPs) and secrete IGFBPs in culture

Cited by 44 publications

References 25 publications

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Insulin-Like Growth Factor-Binding Protein-1 in Human Follicular Fluid: A Marker for Oocyte Maturation

Effect of Somatotropin and/or Equine Chorionic Gonadotropin on Serum and Follicular Insulin-Like Growth Factor I and Insulin-Like Growth Factor Binding Proteins in Cattle1

Insulin‐like growth factor‐binding protein‐2 inhibits proliferation of human embryonic kidney fibroblasts and of IGF‐responsive colon carcinoma cell lines

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