Human gingival fibroblasts are critical in sustaining inflammation in periodontal disease

Ara, Toshiaki; Kurata, Kazuyuki; Hirai, Kaname; Uchihashi, Takayuki; Uematsu, Takashi; Imamura, Yasuhiro; Furusawa, Kiyofumi; Kurihara, Saburo; Wang, Pao-Li

doi:10.1111/j.1600-0765.2007.01041.x

Cited by 180 publications

(146 citation statements)

References 32 publications

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“…Cytokines are produced by resident cells, such as epithelial cells and fibroblasts, and phagocytes (neutrophils and macrophages) in the acute phase and early chronic phase of inflammation, and by immune cells (lymphocytes) in adaptive immunity (Ara et al 2009). After microbial recognition, cytokines of the innate response, including TNFa, IL-1b and IL-6, are the first secreted in periodontal disease pathogenesis (Garlet 2010).…”

Section: Innate Immunity -The Inflammatory Responsementioning

confidence: 99%

Infection and inflammatory mechanisms

Dyke

Winkelhoff

2013

Journal of Periodontology

146

123

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This introductory article examines the potential mechanisms that may play a role in the associations between periodontitis and the systemic conditions being considered in the EFP/AAP Workshop in Segovia, Spain. Three basic mechanisms have been postulated to play a role in these interactions; metastatic infections, inflammation and inflammatory injury, and adaptive immunity. The potential role of each alone and together is considered in in vitro and animal studies and in human studies when available. This is not a systematic or critical review, but rather an overview of the field to set the stage for the critical reviews in each of the working groups.

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Section: Innate Immunity -The Inflammatory Responsementioning

confidence: 99%

Infection and inflammatory mechanisms

Dyke

Winkelhoff

2013

Journal of Periodontology

146

123

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“…From these indications, we considered that hangeshashinto could be useful for periodontal disease. 18) in the presence of LPS, these mediators and cytokines in periodontal tissues are thought to be…”

Section: Introductionmentioning

confidence: 99%

Preventive Effects of a Kampo Medicine, Hangeshashinto on Inflammatory Responses in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Nakazono

Ara

Fujinami

et al. 2010

J. Hard Tissue Biology.

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In the present study, we investigated the effects of a Kampo medicine hangeshashinto (TJ-14) on the production of prostaglandin E 2 (PGE 2 ), interleukin (IL)-6 and IL-8 by human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis. HGFs proliferation was dose-dependently decreased with hangeshashinto at days 3 and 7. However, treatment with LPS (10 ng/ml), hangeshashinto (up to 1 mg/ml) and their combinations for 24 h did not affect the viability of HGFs. Hangeshashinto dose-dependently suppressed LPS-induced PGE 2 production but did not alter basal PGE 2 level. Hangeshashinto weakly decreased LPS-induced IL-6 and IL-8 productions. Hangeshashinto decreased cyclooxygenase (COX)-1 and COX-2 activities to approximately 60% at 1 mg/ml. Hangeshashinto decreased cytoplasmic phospholipase A 2 (cPLA 2 ) expression and LPS-induced COX-2 expression but not affected annexin1 expression. Hangeshashinto weakly suppressed LPS-induced extracellular signal-regulated kinase (ERK) phosphorylation, which is known to lead to ERK activation and cPLA 2 phosphorylation. These results suggest that hangeshashinto decreased PGE 2 production by (1) suppression of cPLA 2 and LPS-induced COX-2 expression, and to a lesser extent, (2) inhibition of COX-2 activity and (3) inhibition of cPLA 2 phosphorylation and its activation via inhibition of ERK phosphorylation. Moreover, it is also suggested that hangeshashinto may be useful to improve gingival inflammation in periodontal disease.

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“…Human gingival fibroblasts (HGFs) are the most abundant cell type in gingival connective tissue and produce collagen while sustaining the structure or homeostasis of the periodontal tissue [7][8][9] . Placental extracts are produced by the purification of placenta through a process involving dialysis, heat treatment, and hydrolysis; they contain various biologically active substances such as amino acids, peptides, glycosaminoglycans, minerals, and growth factors 10) .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Collagen Type-1 Production and Anti-Inflammatory Activities of Human Placental Extracts in Human Gingival Fibroblasts

Akagi¹,

Imamura

Makita

et al. 2016

J. Hard Tissue Biology.

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Placental extracts contain various bioactive agents and are thought to be a prospective medicine for oral diseases. However, detailed information regarding their mechanisms with respect to treatment of periodontal diseases is not available, thereby hindering their reliable application in dentistry. In this study, we demonstrated that human placental extracts increased collagen type-1 production, which is linked to the regenerative capabilities of periodontal tissue, on primary human gingival fibroblasts in vitro. Generally, deterioration of periodontal tissue occurs when various types of cytokines are released by periodontal tissue cells in response to stimulation by plaque. However, we exhibited that human placental extracts hindered the pro-inflammatory cytokines such as interleukin (IL)-6 and IL-8 secretion from primary human gingival fibroblasts in vitro. The results contribute to understanding a therapeutic mechanism underlying the effect of human placenta extracts against periodontal disease by modulating the function of human gingival fibroblasts.

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Human gingival fibroblasts are critical in sustaining inflammation in periodontal disease

Abstract: These results demonstrate that HGFs do not show LPS tolerance and suggest that this characteristic of HGFs sustains the inflammatory response in the presence of virulence factors.

Cited by 180 publications

References 32 publications

Infection and inflammatory mechanisms

Infection and inflammatory mechanisms

Preventive Effects of a Kampo Medicine, Hangeshashinto on Inflammatory Responses in Lipopolysaccharide-Treated Human Gingival Fibroblasts

Evaluation of Collagen Type-1 Production and Anti-Inflammatory Activities of Human Placental Extracts in Human Gingival Fibroblasts

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