Human galectin‑3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions <i>in vivo</i>

Gál, Péter; Vasilenko, Tomáš; Kováč, Ivan; Čoma, Matúš; Jakubčo, Ján; Jakubčová, Martina; Peržeľová, Vlasta; Urban, Lukáš; Kolář, Michal; Sabol, František; Luczy, Ján; Novotný, Martin; Majerník, Jaroslav; Gabius, Hans‐Joachim; Smetana, Karel

doi:10.3892/mmr.2020.11738

Cited by 8 publications

(7 citation statements)

References 61 publications

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“…The set of primary and secondary antibodies applied in the analysis is shown in Table S6 . The analysis was performed as described previously 46 . Briefly, tested specimens were fixed with 2% buffered paraformaldehyde (pH = 7.2) for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Pham

Jenčová

Kaňuchová

et al. 2021

Sci Rep

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Active wound dressings are attracting extensive attention in soft tissue repair and regeneration, including bacteria-infected skin wound healing. As the wide use of antibiotics leads to drug resistance we present here a new concept of wound dressings based on the polycaprolactone nanofiber scaffold (NANO) releasing second generation lipophosphonoxin (LPPO) as antibacterial agent. Firstly, we demonstrated in vitro that LPPO released from NANO exerted antibacterial activity while not impairing proliferation/differentiation of fibroblasts and keratinocytes. Secondly, using a mouse model we showed that NANO loaded with LPPO significantly reduced the Staphylococcus aureus counts in infected wounds as evaluated 7 days post-surgery. Furthermore, the rate of degradation and subsequent LPPO release in infected wounds was also facilitated by lytic enzymes secreted by inoculated bacteria. Finally, LPPO displayed negligible to no systemic absorption. In conclusion, the composite antibacterial NANO-LPPO-based dressing reduces the bacterial load and promotes skin repair, with the potential to treat wounds in clinical settings.

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Section: Methodsmentioning

confidence: 99%

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Pham

Jenčová

Kaňuchová

et al. 2021

Sci Rep

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“…Galectin-3 and galectin-1 are up regulated during hypoxic conditions and act to promote EMT, collective migration, and ultimately breast cancer metastasis (61)(62)(63). The tumor environment that facilitates the activation of metastasis has been described as "a wound that never heals" and is consistent with the promotional role of galectins in wound healing and associated collagen organization (64)(65)(66). Current literature has shown that breast polyploid giant cancer cells or syncytial (multinucleated) cells, are associated with increases in EMT, N-cadherin, invasion, and metastasis in patient tumor biopsies, as well as MCF-7 cells, and MDA-MB-231 breast cancer cells (67).…”

Section: Galectinsmentioning

confidence: 91%

Role of Galectins in Metastatic Breast Cancer

Grazier

Sylvester

2022

Breast Cancer

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Galectins play a role in mammary morphogenesis and are expressed in all breast cancer subtypes.Galectins bind with glycoconjugates, as well as carbohydrate-independent targets in both the cytosolic and nuclear subcellular fractions. All tissues express galectins and galectins bind to numerous targets localized in different subcellular domains, including cell membrane, nucleus, mitochondria, and extracellular matrix. Galectin-3 and galectin-1 are associated with breast cancer progression and metastasis and will be the focus of this chapter. The chapter highlights mechanisms involved in galectins' role in modulating metastatic breast cancer phenotype.Immunocytochemistry analysis of galectin-3 in human breast cancer cells is used to illustrate galectin expression in the metastatic phenotype.Note to the Reader: This chapter is part of the book Breast Cancer (ISBN: 978-0-6453320-3-2), scheduled for publication in July 2022. The book is being published by Exon Publications,

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“…Of course, the detected indication for enhanced bioactivity in our growth assays provides incentive for testing stable heterodimers in settings where two natural galectins are known to cooperate, e.g. in wound healing (Cao et al 2002;Dvoránková et al 2011;Gál et al 2021). Suppression of tumor growth by Gal-7 gene transfer (Ueda et al 2004) may further benefit from firmly locking the quaternary structure.…”

Section: Intestinal Villimentioning

confidence: 92%

Imitating evolution’s tinkering by protein engineering reveals extension of human galectin-7 activity

Ludwig

Michalak

Gabba

et al. 2021

Histochem Cell Biol

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Wild-type lectins have distinct types of modular design. As a step to explain the physiological importance of their special status, hypothesis-driven protein engineering is used to generate variants. Concerning adhesion/growth-regulatory galectins, non-covalently associated homodimers are commonly encountered in vertebrates. The homodimeric galectin-7 (Gal-7) is a multifunctional context-dependent modulator. Since the possibility of conversion from the homodimer to hybrids with other galectin domains, i.e. from Gal-1 and Gal-3, has recently been discovered, we designed Gal-7-based constructs, i.e. stable (covalently linked) homo- and heterodimers. They were produced and purified by affinity chromatography, and the sugar-binding activity of each lectin unit proven by calorimetry. Inspection of profiles of binding of labeled galectins to an array-like platform with various cell types, i.e. sections of murine epididymis and jejunum, and impact on neuroblastoma cell proliferation revealed no major difference between natural and artificial (stable) homodimers. When analyzing heterodimers, acquisition of altered properties was seen. Remarkably, binding properties and activity as effector can depend on the order of arrangement of lectin domains (from N- to C-termini) and on the linker length. After dissociation of the homodimer, the Gal-7 domain can build new functionally active hybrids with other partners. This study provides a clear direction for research on defining the full range of Gal-7 functionality and offers the perspective of testing applications for engineered heterodimers.

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Human galectin‑3: Molecular switch of gene expression in dermal fibroblasts in vitro and of skin collagen organization in open wounds and tensile strength in incisions in vivo

Cited by 8 publications

References 61 publications

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Novel lipophosphonoxin-loaded polycaprolactone electrospun nanofiber dressing reduces Staphylococcus aureus induced wound infection in mice

Role of Galectins in Metastatic Breast Cancer

Imitating evolution’s tinkering by protein engineering reveals extension of human galectin-7 activity

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