Human Follicular Fluid Levels of Calcium, Copper and Zinc

Ng, Soon-Chye; Karunanithy, R.; Edirisinghe, W. Rohini; Roy, A.C.; Wong, P. C.; Ratnam, S. S.

doi:10.1159/000298848

Cited by 22 publications

(19 citation statements)

References 14 publications

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“…Similarly, Zn media supplementation (3.6-19 μmol) was associated with better quality mouse embryos [38,39], yet higher concentrations (25 μmol) were embryotoxic [39]. Still, an earlier study of 33 human follicles found no association between Zn and oocyte fertilization during IVF [40]. The mean FF Zn (720 μg/L) in that study was higher than the maximum value measured in this study (687 μg/L).…”

Section: Discussioncontrasting

confidence: 64%

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

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Purpose A hypothesis-generating pilot study exploring associations between essential trace elements measured in follicular fluid (FF) and urine and in vitro fertilization (IVF) endpoints. Methods We recruited 58 women undergoing IVF between 2007 and 2008, and measured cobalt, chromium, copper, manganese, molybdenum, and zinc in FF (n = 46) and urine (n = 45) by inductively coupled plasma mass spectrometry (ICP-MS). We used multivariable regression models to assess the impact of FF and urine trace elements on IVF outcomes, adjusted for age, body mass index, race, and cigarette smoking.Results Trace elements were mostly present at lower concentrations in FF than in urine. The average number of oocytes retrieved was positively associated with higher urine cobalt, chromium, copper, and molybdenum concentrations. FF chromium and manganese were negatively associated with the proportion of mature oocytes, yet urine manganese had a positive association. FF zinc was inversely associated with average oocyte fertilization. Urine trace elements were significant positive predictors for the total number of embryos generated. FF copper predicted lower embryo fragmentation while urine copper was associated with higher embryo cell number and urine manganese with higher embryo fragmentation. No associations were detected for implantation, pregnancy, or live birth. Conclusions Our results suggest the importance of trace elements in both FF and urine for intermediate, although not necessarily clinical, IVF endpoints. The results differed using FF or urine biomarkers of exposure, which may have implications for the design of clinical and epidemiologic investigations. These initial findings will form the basis of a more definitive future study.

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Section: Discussioncontrasting

confidence: 64%

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Ingle

Bloom

Parsons

et al. 2016

J Assist Reprod Genet

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show abstract

“…However, the study measured only plasma zinc concentrations, which only reflect short term zinc turnover. A further study of the zinc levels in the follicular fluid of 33 follicles taken during ART found no difference in the levels between follicles of different sizes, and those that yielded oocytes that were successful and unsuccessful in fertilisation [40], but no additional confirmatory studies have been undertaken.…”

Section: Discussionmentioning

confidence: 97%

Endocrine disruptor & nutritional effects of heavy metals in ovarian hyperstimulation

Dickerson¹,

Sathyapalan

Knight

et al. 2011

J Assist Reprod Genet

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Purpose There is increasing concern that environmental chemicals have a direct effect on fertility. Heavy metals such as mercury have been shown to affect various organ systems in humans including nervous system and skin, however they could also act as endocrine disrupting chemicals adversely affecting fertility. Metals such as zinc and selenium are essential micronutrients with diverse functions that may be important for reproductive outcomes. We measured mercury, zinc and selenium levels in the hair, a reliable reflection of long term environmental exposure and dietary status, to correlate with the outcome of ovarian hyperstimulation for in vitro fertilisation (IVF) treatment. Methods We analysed the hair of 30 subfertile women for mercury, zinc and selenium using inductively coupled mass spectrometry. Each woman underwent one cycle of IVF treatment. Correlation between the levels of these trace metals and treatment outcomes was investigated.Results Thirty women were recruited with mean (±SD) age of 32.7(4.4) years and BMI of 25.4(5.0)kg/m 2 . Hair mercury concentration showed a negative correlation with oocyte yield (p<0.05,βcoefficient 0.38) and follicle number (p=0.03,β coefficient0.19) after ovarian stimulation. Zinc and selenium levels in hair correlated positively with oocyte yield after ovarian stimulation (p<0.05,β coefficient0.15) and (p=0.03,β coefficient0.21) respectively. Selenium levels in hair correlated significantly with follicle number following stimulation (p=0.04, βcoefficient0.22). There was no correlation between mercury, zinc and selenium in hair and their corresponding serum levels. Conclusion These data suggest that mercury had a deleterious effect whilst there was a positive effect for zinc and selenium in the ovarian response to gonadotrophin therapy for IVF. Hair analysis offers a novel method of investigating the impact of long-term exposure to endocrine disruptors and nutritional status on reproductive outcomes. Keywords IVF . Heavy metals . Endocrine disruptorsSubfertility affects approximately 1 in 6 couples in the developed world [1], a prevalence that is likely to increase as more women delay childbirth to a later age [2]; approximately 28% of couples investigated for subfertility have a diagnosis of 'unexplained subfertility' [3]. Current treatment largely consists of assisted reproductive techniques (ART) such as IVF or IVF-ICSI, often regardless of the underlying cause. The average success rate of 25-30% for ART in Europe remains unchanged over recent years [4], prompting the search for additional factors which may influence fertility treatments and optimise success, and the identification of adverse factors such as endocrine disrupting chemicals.Capsule Mercury concentration in hair had a deleterious effect where as zinc and selenium had a beneficial effect in ovarian response to gonadotrophin therapy for IVF.

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“…Studies on the effects of zinc deficiency in women with IVF are scarce. Ng et al [10] investigated zinc levels in follicular fluid of 33 women undergoing IVF in Singapore and did not observe any correlation between follicular fluid zinc concentration and follicle volume. Jameson [19] reported longstanding infertility in seven, normal sexually developed women with celiac disease.…”

Section: Discussionmentioning

confidence: 98%

“…In addition, magnesium plays a protective role in the developing fetus brain [9]. Calcium has a basic function in neurotransmitter secretion and magnesium blocks the entrance of calcium into cells [10]. Enzymatic and nonenzymatic antioxidants are also essential for inhibition of free radical production related to oocyte maturation and rupture [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Multivitamin/Mineral Supplementation on Trace Element Levels in Serum and Follicular Fluid of Women Undergoing in Vitro Fertilization (IVF)

Özkaya

Nazıroğlu

Barak

et al. 2010

Biol Trace Elem Res

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We investigated effects of multivitamin/mineral supplementation on element levels in serum and follicular fluid of women undergoing IVF. We used three groups in this study. The first group was used as an age-matched and nonpregnant control (n = 13). Group 2 (n = 30) constituted the IVF group and women in the third group who were undergoing IVF also received a multivitamin/mineral tablet daily for 45 days. Follicular fluid and serum selenium and zinc levels and follicular fluid copper levels were lower in IVF patients than in controls although follicular fluid aluminum and iron levels were higher in IVF patients than in controls. However, follicular fluid and serum aluminum, copper, zinc and selenium levels, and serum magnesium levels were higher in the multivitamin/mineral group than in the IVF group although follicular fluid iron levels were lower in the multivitamin/mineral group than in the IVF group. In conclusion, we observed that copper, zinc, and selenium in serum and follicular fluid decreased in women undergoing IVF. Multivitamin/mineral supplementation in serum and follicular fluid of women undergoing IVF normalized the trace element levels.

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Human Follicular Fluid Levels of Calcium, Copper and Zinc

Cited by 22 publications

References 14 publications

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Associations between IVF outcomes and essential trace elements measured in follicular fluid and urine: a pilot study

Endocrine disruptor & nutritional effects of heavy metals in ovarian hyperstimulation

Effects of Multivitamin/Mineral Supplementation on Trace Element Levels in Serum and Follicular Fluid of Women Undergoing in Vitro Fertilization (IVF)

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